Disinfect Porphyromonas gingivalis Biofilm on Titanium Surface with Combined Application of Chlorhexidine and Antimicrobial Photodynamic Therapy

2018 ◽  
Vol 95 (3) ◽  
pp. 839-845 ◽  
Author(s):  
Zhiyu Cai ◽  
Yijun Li ◽  
Yanhuang Wang ◽  
Shuai Chen ◽  
Shan Jiang ◽  
...  
2020 ◽  
Vol 32 ◽  
pp. 102033
Author(s):  
Yutaro Kitanaka ◽  
Yasuo Takeuchi ◽  
Koichi Hiratsuka ◽  
Nay Aung ◽  
Yuriko Sakamaki ◽  
...  

Author(s):  
Pier Poli ◽  
Francisley Avila Souza ◽  
Mattia Manfredini ◽  
Carlo Maiorana ◽  
Mario Beretta

Not required for Clinical case letters according to the authors' guidelines.


Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 869
Author(s):  
Beatriz Müller Nunes Souza ◽  
Juliana Guerra Pinto ◽  
André Henrique Correia Pereira ◽  
Alejandro Guillermo Miñán ◽  
Juliana Ferreira-Strixino

Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.


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