Activation of the General Stress Response ofBacillus subtilisby Visible Light

2015 ◽  
Vol 91 (5) ◽  
pp. 1032-1045 ◽  
Author(s):  
Jeroen B. van der Steen ◽  
Klaas J. Hellingwerf
Keyword(s):  
Stress Response ◽  
Visible Light ◽  
General Stress Response ◽  
General Stress

scholarly journals Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light

2019 ◽  
Vol 112 (2) ◽  
pp. 442-460 ◽  
Author(s):  
Aretha Fiebig ◽  
Lydia M. Varesio ◽  
Xiomarie Alejandro Navarreto ◽  
Sean Crosson
Keyword(s):  
Stress Response ◽  
Visible Light ◽  
General Stress Response ◽  
General Stress ◽  
Erythrobacter Litoralis

scholarly journals Regulation of the Erythrobacter litoralis DSM 8509 general stress response by visible light

2019 ◽  
Author(s):  
Aretha Fiebig ◽  
Lydia M. Varesio ◽  
Xiomarie Alejandro Navarreto ◽  
Sean Crosson
Keyword(s):  
Stress Response ◽  
Visible Light ◽  
Genetic Model ◽  
Multiple Stressors ◽  
Histidine Kinases ◽  
Diverse Range ◽  
General Stress Response ◽  
General Stress ◽  
Sensor Kinases ◽  
Erythrobacter Litoralis

SUMMARYExtracytoplasmic function (ECF) sigma factors are a major class of environmentally-responsive transcriptional regulators. In Alphaproteobacteria the ECF sigma factor, σEcfG, activates general stress response (GSR) transcription and protects cells from multiple stressors. A phosphorylation-dependent protein partner switching mechanism, involving HWE/HisKA_2-family histidine kinases, underlies σEcfG activation. The identity of these sensor kinases and the signals that regulate them remain largely uncharacterized. We have developed the aerobic anoxygenic photoheterotrophic (AAP) bacterium, Erythrobacter litoralis DSM 8509, as a comparative genetic model to investigate GSR regulation. Using this system, we sought to define the contribution of visible light and a photosensory HWE kinase, LovK, to GSR transcription. We identified three HWE kinases that collectively regulate GSR: gsrK and lovK are activators, while gsrP is a repressor. GSR transcription is higher in the dark than light, and the opposing activities of gsrK and gsrP are sufficient to achieve light-dependent differential transcription. In the absence of gsrK and gsrP, lovK alone is sufficient to regulate GSR transcription in response to light. This regulation requires a photochemically active LOV domain in LovK. Our studies establish a role for visible light and HWE kinases in light-dependent regulation of GSR transcription in E. litoralis, an AAP species.GRAPHICAL ABSTRACTABBREVIATED SUMMARYGeneral stress response (GSR) systems protect bacteria from a diverse range of physical and chemical stressors. We have developed Erythrobacter litoralis as a new genetic model to study GSR in Alphaproteobacteria and show that three HWE-family histidine kinases collectively regulate GSR transcription via σEcfG. Visible light is a GSR regulatory signal in E. litoralis, and LovK is a blue-light photosensor kinase that functions as a dark activated GSR regulator.

scholarly journals Mtl1 Is Required to Activate General Stress Response through Tor1 and Ras2 Inhibition under Conditions of Glucose Starvation and Oxidative Stress

2010 ◽  
Vol 285 (25) ◽  
pp. 19521-19531 ◽  
Author(s):  
Mima Ivanova Petkova ◽  
Nuria Pujol-Carrion ◽  
Javier Arroyo ◽  
Jesús García-Cantalejo ◽  
Maria Angeles de la Torre-Ruiz
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Glucose Starvation ◽  
General Stress Response ◽  
General Stress ◽  
And Oxidative Stress

scholarly journals Modeling the functioning of YtvA in the general stress response in Bacillus subtilis

2013 ◽  
Vol 9 (9) ◽  
pp. 2331 ◽  
Author(s):  
Jeroen B. van der Steen ◽  
Yusuke Nakasone ◽  
Johnny Hendriks ◽  
Klaas J. Hellingwerf
Keyword(s):  
Bacillus Subtilis ◽  
Stress Response ◽  
General Stress Response ◽  
General Stress

scholarly journals Development and optimization of an EGFP-based reporter for measuring the general stress response inListeria monocytogenes

Bioengineered ◽  
2012 ◽  
Vol 3 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Marta Utratna ◽  
Eoin Cosgrave ◽  
Claas Baustian ◽  
Rhodri Ceredig ◽  
Conor O’Byrne
Keyword(s):  
Stress Response ◽  
General Stress Response ◽  
General Stress

scholarly journals Complex general stress response regulation in Sphingomonas melonis Fr1 revealed by transcriptional analyses

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lisa Gottschlich ◽  
Petra Geiser ◽  
Miriam Bortfeld-Miller ◽  
Christopher M. Field ◽  
Julia A. Vorholt
Keyword(s):  
Stress Response ◽  
General Stress Response ◽  
General Stress
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