scholarly journals A Fiberoptic (Photodynamic Therapy Type) Device with a Photosensitizer and Singlet Oxygen Delivery Probe Tip for Ovarian Cancer Cell Killing

2013 ◽  
Vol 89 (4) ◽  
pp. 936-941 ◽  
Author(s):  
Dorota Bartusik ◽  
David Aebisher ◽  
Ashwini Ghogare ◽  
Goutam Ghosh ◽  
Inna Abramova ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Cancer Cell ◽  
Oxygen Delivery ◽  
Ovarian Cancer Cell ◽  
Cell Killing ◽  
Type Device

Pre-clinical models of cellular therapy combined with cytokines demonstrate in vitro and in vivo ovarian cancer cell killing

2012 ◽  
Vol 125 ◽  
pp. S45
Author(s):  
S. Ingersoll ◽  
S. Ahmad ◽  
G. Stoltzfus ◽  
M. Merchant ◽  
A. Ahmed ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cell ◽  
Cellular Therapy ◽  
Ovarian Cancer Cell ◽  
Cell Killing ◽  
Clinical Models

scholarly journals Synergy between Photodynamic Therapy and Dactinomycin Chemotherapy in 2D and 3D Ovarian Cancer Cell Cultures

2020 ◽  
Vol 21 (9) ◽  
pp. 3203 ◽  
Author(s):  
Layla Mohammad Hadi ◽  
Elnaz Yaghini ◽  
Alexander J. MacRobert ◽  
Marilena Loizidou
Keyword(s):  
Ovarian Cancer ◽  
Photodynamic Therapy ◽  
Cell Lines ◽  
Cancer Cell ◽  
Low Dose ◽  
Ovarian Cancer Cell ◽  
3D Culture ◽  
Ovarian Cancer Cell Lines ◽  
Cell Kill ◽  
2D And 3D

In this study we explored the efficacy of combining low dose photodynamic therapy using a porphyrin photosensitiser and dactinomycin, a commonly used chemotherapeutic agent. The studies were carried out on compressed collagen 3D constructs of two human ovarian cancer cell lines (SKOV3 and HEY) versus their monolayer counterparts. An amphiphilc photosensitiser was employed, disulfonated tetraphenylporphine, which is not a substrate for ABC efflux transporters that can mediate drug resistance. The combination treatment was shown to be effective in both monolayer and 3D constructs of both cell lines, causing a significant and synergistic reduction in cell viability. Compared to dactinomycin alone or PDT alone, higher cell kill was found using 2D monolayer culture vs. 3D culture for the same doses. In 3D culture, the combination therapy resulted in 10 and 22 times higher cell kill in SKOV3 and HEY cells at the highest light dose compared to dactinomycin monotherapy, and 2.2 and 5.5 times higher cell kill than PDT alone. The combination of low dose PDT and dactinomycin appears to be a promising way to repurpose dactinomycin and widen its therapeutic applications.

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