Acetaminophen elevates unbound bilirubin levels by the glucose oxidase‐peroxidase method

2021 ◽  
Author(s):  
Shintaro Ichimura ◽  
Hiroki Kakita ◽  
Shimpei Asai ◽  
Mari Mori ◽  
Satoru Takeshita ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Unbound Bilirubin

scholarly journals A Novel Method for Measuring Serum Unbound Bilirubin Levels Using Glucose Oxidase–Peroxidase and Bilirubin-Inducible Fluorescent Protein (UnaG): No Influence of Direct Bilirubin

2020 ◽  
Vol 21 (18) ◽  
pp. 6778
Author(s):  
Sota Iwatani ◽  
Keiji Yamana ◽  
Hajime Nakamura ◽  
Kosuke Nishida ◽  
Takeshi Morisawa ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Fluorescent Protein ◽  
Linear Regression Analysis ◽  
Direct Bilirubin ◽  
Molar Ratio ◽  
Total Serum ◽  
Indirect Bilirubin ◽  
Unbound Bilirubin ◽  
Novel Method ◽  
Highly Correlated

The glucose oxidase–peroxidase (GOD–POD) method used to measure serum unbound bilirubin (UB) suffers from direct bilirubin (DB) interference. Using a bilirubin-inducible fluorescent protein from eel muscle (UnaG), a novel GOD–POD–UnaG method for measuring UB was developed. Newborn sera with an indirect bilirubin/albumin (iDB/A) molar ratio of <0.5 were classified into four groups of DB/total serum bilirubin (TB) ratios (<5%, 5–10%, 10–20%, and ≥20%), and the correlation between the UB levels and iDB/A ratio was examined. Linear regression analysis was performed to compare UB values from both methods with the iDB/A ratio from 38 sera samples with DB/TB ratio <5% and 11 samples with DB/TB ratio ≥5%. The correlation coefficient (r) between UB values and the iDB/A ratio for the GOD–POD method was 0.8096 (DB/TB ratio <5%, n = 239), 0.7265 (5–10%, n = 29), 0.7165 (10–20%, n = 17), and 0.4816 (≥20%, n = 16). UB values using the GOD–POD–UnaG method highly correlated with the iDB/A ratio in both <5% and ≥5% DB/TB ratio sera (r = 0.887 and 0.806, respectively), whereas a low correlation (r = 0.428) occurred for ≥5% DB/TB ratio sera using the GOD–POD method. Our GOD–POD–UnaG method can measure UB levels regardless of the presence of DB.

Effects of metal ions on simultaneous production of glucose oxidase and catalase by Aspergillus niger

2001 ◽  
Vol 32 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J.-Z. Liu ◽  
Y.-Y. Huang ◽  
J. Liu ◽  
L.-P. Weng ◽  
L.-N. Ji
Keyword(s):  
Aspergillus Niger ◽  
Metal Ions ◽  
Glucose Oxidase ◽  
Simultaneous Production

Temperature-induced amperometric glucose biosensor based on a poly(N-vinylcaprolactam)/graphene oxide composite film

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1960-1967 ◽  
Author(s):  
Chao Chen ◽  
Pengcheng Zhao ◽  
Meijun Ni ◽  
Chunyan Li ◽  
Yixi Xie ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Composite Film ◽  
Glucose Oxidase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Glucose Biosensor ◽  
Carbon Electrode ◽  
Oxide Composite

A temperature-induced sensing film consisting of poly(N-vinylcaprolactam) (PVCL), graphene oxide (GO) and glucose oxidase (GOD) was fabricated and used to modify a glassy carbon electrode (GCE).

Bilirubin/albumin (B/A) ratios correlate with unbound bilirubin levels in preterm infants

2021 ◽  
Author(s):  
Shinya Abe ◽  
Kazumichi Fujioka ◽  
Ruka Nakasone ◽  
Shutaro Suga ◽  
Mariko Ashina ◽  
...  
Keyword(s):  
Preterm Infants ◽  
Unbound Bilirubin
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