Skin preparation for prevention of peripheral blood culture contamination in children

2019 ◽  
Vol 61 (7) ◽  
pp. 647-651 ◽  
Author(s):  
Toshifumi Yodoshi ◽  
Shinichiro Ueda ◽  
Ran D Goldman
Keyword(s):  
Blood Culture ◽  
Peripheral Blood ◽  
Skin Preparation ◽  
Peripheral Blood Culture

Distinguishing Sepsis From Blood Culture Contamination in Young Infants With Blood Cultures Growing Coagulase-Negative Staphylococci

PEDIATRICS ◽  
1990 ◽  
Vol 86 (2) ◽  
pp. 157-162
Author(s):  
Joseph W. St Geme ◽  
Louis M. Bell ◽  
Stephen Baumgart ◽  
Carl T. D'Angio ◽  
Mary Catherine Harris
Keyword(s):  
Blood Culture ◽  
Peripheral Blood ◽  
Clinical Information ◽  
Study Groups ◽  
Blood Cultures ◽  
High Risk Population ◽  
Coagulase Negative Staphylococci ◽  
Peripheral Blood Culture ◽  
Young Infants ◽  
Initial Culture

Coagulase-negative staphylococci represent the most common cause of serious nosocomial infection in many intensive care nurseries. However, these organisms are also common blood culture contaminants. To determine the value of quantitative blood cultures in distinguishing sepsis from culture contamination, we reviewed records of all infants in our nurseries who had peripheral blood isolates of coagulase-negative staphylococci during a 3-year period. Twenty-three episodes of sepsis were identified in 21 infants, and 10 infants had blood culture contamination. Colony counts from the initial peripheral blood culture were significantly different for the two study groups (P < .001). In 9 of 23 episodes of sepsis, the initial peripheral blood culture grew >100 colony-forming units (cfu) per mL. In the other 14 episodes, the initial culture yielded ≤50 cfu/mL. All 10 infants with culture contamination had colony counts of <50 cfu/mL, and in 9 the initial peripheral blood culture grew <20 cfu/mL. Infants with sepsis, including those with colony counts of ≤50 cfu/mL, were significantly more likely to have a central catheter or an abnormal hematologic value or both (P < .05). Infants who lacked these clinical features were more likely to have contamination. We conclude that quantitative blood cultures in conjunction with specific clinical information may distinguish sepsis from culture contamination with coagluase-negative staphylococci in young infants. In addition, low colony-count growth should not be ignored as contamination in this high-risk population.

Accuracy of procalcitonin for diagnosing peripheral blood culture contamination among patients with positive blood culture for potential contaminants

Infection ◽  
2021 ◽  
Author(s):  
Clémence Berthezène ◽  
Nejla Aissa ◽  
Anne Elisabeth Manteaux ◽  
Jean-Louis Guéant ◽  
Abderrahim Oussalah ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Peripheral Blood ◽  
Peripheral Blood Culture

Peripheral blood culture contamination in adults and venepuncture technique: prospective cohort study

2007 ◽  
Vol 61 (4) ◽  
pp. 509-513 ◽  
Author(s):  
A Qamruddin ◽  
N Khanna ◽  
D Orr
Keyword(s):  
Cohort Study ◽  
Blood Culture ◽  
Prospective Cohort Study ◽  
Peripheral Blood ◽  
Prospective Cohort ◽  
Univariate Analysis ◽  
Blood Collection ◽  
Peripheral Blood Culture ◽  
Protocol Compliance ◽  
Hospital Protocol

Aims:To test the hypothesis that compliance with a hospital protocol on peripheral blood culture (PBC) collection in adults is associated with a reduction in PBC contamination, and to investigate likely contributing factors for contamination.Methods:A prospective cohort study was conducted, utilising data collection by participant questionnaire completion, and utilising bacteriology laboratory results on PBCs. Participants were all healthcare workers involved in obtaining PBCs from adults.Results:1460 PBCs with questionnaires were received. Contamination among the 1460 PBCs as a whole was 8.8%. 766 of the questionnaires were sufficiently complete to allow analysis of blood culture contamination in relation to protocol compliance. Among these, protocol compliance was 30% and contamination was 8.0%. When the protocol was complied with, 2.6% of PBCs were contaminated, but when the protocol was not followed, contamination was significantly higher at 10.3% (OR 4.35, 95% CI 1.84 to 12.54). Univariate analysis on all 1460 PBCs suggested that the site for blood collection, and disinfection of the venepuncture site were important factors in PBC contamination: when no venepuncture site disinfection was performed, contamination was significantly higher than when alcohol was used (5.1% versus 15.8%, OR 3.46, 95% CI 2.01 to 5.97); when a PBC collection site other than a fresh peripheral vein was used, contamination was significantly higher (7.3% versus 12.0%, OR 1.75, 95% CI 1.03 to 2.96).Conclusions:Compliance with a hospital protocol on PBC collection technique in adults significantly reduces blood culture contamination.

THE SOMATIC KARYOTYPES OF AMERICAN BISON AND DOMESTIC CATTLE

1969 ◽  
Vol 11 (2) ◽  
pp. 243-249 ◽  
Author(s):  
R. Bhambhani ◽  
J. Kuspira
Keyword(s):  
Blood Culture ◽  
Peripheral Blood ◽  
Culture Technique ◽  
Arbitrary System ◽  
Uniform System ◽  
Peripheral Blood Culture

Established karyotypes for bovines are generally lacking and therefore necessitate at least a preliminary arbitrary system of nomenclature. The somatic karyotypes of two bovines (American bison and domestic cattle) were determined utilising a peripheral-blood culture technique. On the basis of a karyotype comparison, the cytotaxonomic relationship between the bison and the domestic cattle is stressed. A preliminary arbitrary system of nomenclature for bovine chromosomes has been suggested. It is hoped that further similar investigations will enable the establishment of a uniform system of nomenclature for bovine chromosomes.

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