Simple chronic colitis model using hypopigmented mice with aHermansky-Pudlak syndrome 5gene mutation

2016 ◽  
Vol 29 (5) ◽  
pp. 578-582 ◽  
Author(s):  
Yumi Itoh ◽  
Yasuo Nagaoka ◽  
Yoshio Katakura ◽  
Hidehisa Kawahara ◽  
Hiroshi Takemori
Keyword(s):  
Chronic Colitis ◽  
Colitis Model

Novel Histone Deacetylase 6 Inhibitor CKD-506 Inhibits NF-κB Signaling in Intestinal Epithelial Cells and Macrophages and Ameliorates Acute and Chronic Murine Colitis

2020 ◽  
Vol 26 (6) ◽  
pp. 852-862 ◽  
Author(s):  
Jung Won Lee ◽  
Soung-Min Lee ◽  
Jaeyoung Chun ◽  
Jong Pil Im ◽  
Su-Kil Seo ◽  
...  
Keyword(s):  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Histone Deacetylase 6 ◽  
Chronic Colitis ◽  
Acute Colitis ◽  
Murine Colitis ◽  
Tnf Α ◽  
Inflammatory Bowel ◽  
Iκbα Phosphorylation ◽  
Colitis Model

Abstract Background Selective blocking of HDAC6 has become a promising strategy in treating inflammatory bowel disease. CKD-506 is a novel isoform-selective inhibitor of histone deacetylase 6. The present study was performed to evaluate the effect of CKD-506 on the NF-κB signaling pathway in intestinal epithelial cells (IECs) and macrophages and on murine models of acute and chronic colitis. Methods RAW264RAW264.7 murine macrophages and COLO 205 human IECs were pretreated with CKD-506 and then stimulated with lipopolysaccharides (LPS). Cytokine expression of TNF-α, interleukin (IL)-6, IL-8, and IL-10 was measured by ELISA. The effect of CKD-506 on NF-κB signaling was evaluated by Western blotting of IκBα phosphorylation/degradation and electrophoretic mobility shift assay. In vivo studies were performed using a dextran sulfate sodium (DSS)–induced acute colitis model, a chronic colitis model in IL-10 knockout mice, and an adoptive transfer model. Colitis was quantified by the disease activity index, colon length, and histopathologic evaluation. Results CKD-506 suppressed the expression of pro-inflammatory cytokines such as IL-6, IL-8, and TNF-α in IECs and macrophages. CKD-506 strongly inhibited IκBα phosphorylation/degradation and the DNA-binding activity of NF-κB. Oral administration of CKD-506 attenuated DSS-induced acute colitis and chronic colitis in IL-10-/- and adoptive transfer models. CKD-506 ameliorated weight loss, disease activity, and histopathologic score in colitis mice and downregulated IκBα phosphorylation and pro-inflammatory cytokine production significantly. Conclusions CKD-506 blocked NF-κB signaling in IECs and macrophages and ameliorated experimental acute and chronic murine colitis models, which suggests that CKD-506 is a promising candidate for inflammatory bowel disease treatment as a small molecular medicine.

scholarly journals Mo1120 – A Trial for the Administration of a Functionally Enhanced Tonsil-Derived Mesenchymal Stem Cells in a Chronic Colitis Model

2019 ◽  
Vol 156 (6) ◽  
pp. S-712-S-713
Author(s):  
Jiyoung Lim ◽  
Sung-Ae Jung ◽  
Yang-Hee Joo ◽  
A Reum Choe ◽  
So Young Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chronic Colitis ◽  
Colitis Model

Su1180 - The Efficacy of Tonsil-Derived Mesenchymal Stem Cells Conditioned Medium in Chronic Colitis Model

2018 ◽  
Vol 154 (6) ◽  
pp. S-495
Author(s):  
Yang-Hee Joo ◽  
Sung-Ae Jung ◽  
Chung Hyun Tae ◽  
So Young Han ◽  
A Reum Choe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Chronic Colitis ◽  
Colitis Model

scholarly journals P068 Evaluation of the therapeutic potential of metformin-pretreated tonsil-derived mesenchymal stem cells in a chronic colitis model

2017 ◽  
Vol 11 (suppl_1) ◽  
pp. S111-S112
Author(s):  
K.E. Lee ◽  
S.-A. Jung ◽  
Y.-H. Joo ◽  
C.H. Tae ◽  
C.M. Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Potential ◽  
Chronic Colitis ◽  
Colitis Model

Protective Effects of Lactococci Strains Delivering Either IL-10 Protein or cDNA in a TNBS-induced Chronic Colitis Model

2014 ◽  
Vol 48 ◽  
pp. S12-S17 ◽  
Author(s):  
Silvina del Carmen ◽  
Rebeca Martín Rosique ◽  
Tessália Saraiva ◽  
Meritxell Zurita-Turk ◽  
Anderson Miyoshi ◽  
...  
Keyword(s):  
Protective Effects ◽  
Chronic Colitis ◽  
Colitis Model

scholarly journals Kag-308, a Novel Orally Active EP4 Agonist, Ameliorates Mucosal Injury on Endoscopic Examination in a Chronic Colitis Model

2011 ◽  
Vol 140 (5) ◽  
pp. S-517
Author(s):  
Tango Handa ◽  
Masahiro Amakawa ◽  
Yoshihide Miyake ◽  
Mihoko Takami ◽  
Takahiko Murata ◽  
...  
Keyword(s):  
Mucosal Injury ◽  
Endoscopic Examination ◽  
Chronic Colitis ◽  
Orally Active ◽  
Colitis Model

scholarly journals The Oxysterol Synthesising Enzyme CH25H Contributes to the Development of Intestinal Fibrosis

2019 ◽  
Vol 13 (9) ◽  
pp. 1186-1200 ◽  
Author(s):  
T Raselli ◽  
A Wyss ◽  
M N Gonzalez Alvarado ◽  
B Weder ◽  
C Mamie ◽  
...  
Keyword(s):  
Immune Responses ◽  
Biological Processes ◽  
Heterotopic Transplantation ◽  
Dextran Sulphate ◽  
Chronic Colitis ◽  
Intestinal Fibrosis ◽  
Transplant Model ◽  
And Oxidative Stress ◽  
Colitis Model ◽  
Transplantation Model

Abstract Intestinal fibrosis and stenosis are common complications of Crohn’s disease [CD], frequently requiring surgery. Anti-inflammatory strategies can only partially prevent fibrosis; hence, anti-fibrotic therapies remain an unmet clinical need. Oxysterols are oxidised cholesterol derivatives with important roles in various biological processes. The enzyme cholesterol 25-hydroxylase [CH25H] converts cholesterol to 25-hydroxycholesterol [25-HC], which modulates immune responses and oxidative stress. In human intestinal samples from CD patients, we found a strong correlation of CH25H mRNA expression with the expression of fibrosis markers. We demonstrate reduced intestinal fibrosis in mice deficient for the CH25H enzyme, using the sodium dextran sulphate [DSS]-induced chronic colitis model. Additionally, using a heterotopic transplantation model of intestinal fibrosis, we demonstrate reduced collagen deposition and lower concentrations of hydroxyproline in CH25H knockouts. In the heterotopic transplant model, CH25H was expressed in fibroblasts. Taken together, our findings indicate an involvement of oxysterol synthesis in the pathogenesis of intestinal fibrosis.

scholarly journals P033 The efficacy of tonsil-derived mesenchymal stem cells conditioned medium in chronic colitis model

2018 ◽  
Vol 12 (supplement_1) ◽  
pp. S109-S109
Author(s):  
A R Choe ◽  
S -A Jung ◽  
Y -H Joo ◽  
C H Tae ◽  
S Y Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Chronic Colitis ◽  
Colitis Model
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