Effect of a Bacillus subtilis strain on flax protection against Fusarium oxysporum and its impact on the root and stem cell walls

2020 ◽  
Vol 44 (1) ◽  
pp. 304-322
Author(s):  
Aline Planchon ◽  
Gaëlle Durambur ◽  
Jean‐Baptiste Besnier ◽  
Carole Plasson ◽  
Bruno Gügi ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stem Cell ◽  
Fusarium Oxysporum ◽  
Cell Walls ◽  
Subtilis Strain

Development of formulations of biological agents for management of root rot of lettuce and cucumber

2000 ◽  
Vol 46 (9) ◽  
pp. 809-816 ◽  
Author(s):  
G A Amer ◽  
R S Utkhede
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Fusarium Oxysporum ◽  
Wheat Bran ◽  
Root Rot ◽  
Room Temperature ◽  
Pythium Aphanidermatum ◽  
Growth And Yield ◽  
Peat Moss ◽  
Subtilis Strain

The effect of various carrier formulations of Bacillus subtilis and Pseudomonas putida were tested on germination, growth, and yield of lettuce and cucumber crops in the presence of Pythium aphanidermatum and Fusarium oxysporum f.sp. cucurbitacearum, respectively. Survival of B. subtilis and P. putida in various carriers under refrigeration (about 0°C) and at room temperature (about 22°C) was also studied. In all carrier formulations, B. subtilis strain BACT-0 survived up to 45 days. After 45 days of storage at room temperature (about 22°C), populations B. subtilis strain BACT-0 were significantly higher in vermiculite, kaolin, and bacterial broth carriers compared with other carriers. Populations of P. putida were significantly higher in vermiculite, peat moss, wheat bran, and bacterial broth than in other carriers when stored either under refrigeration (about 0°C) or at room temperature (about 22°C) for 15 or 45 days. Germination of lettuce seed was not affected in vermiculite, talc, kaolin, and peat moss carriers, but germination was significantly reduced in alginate and bacterial broth carriers of B. subtilis compared to the non-treated control. Germination of cucumber seed was not affected by any of the carriers. Significantly higher fresh lettuce and root weights were observed in vermiculite and kaolin carriers of B. subtilis compared with P. aphanidermatum-inoculated control plants. Lettuce treated with vermiculite, and kaolin carriers of B. subtilis, or non-inoculated control lettuce plants had significantly lower root rot ratings than talc, peat moss, bacterial broth, and P. aphanidermatum-inoculated control plants. Growth and yield of cucumber plants were significantly higher in vermiculite-based carrier of P. putida than the other carriers and Fusarium oxysporum f.sp. cucurbitacearum-inoculated plants.Key words: Bacillus subtilis, Pseudomonas putida, Fusarium oxysporum f.sp. cucurbitacearum, Pythium aphanidermatum, talc, vermiculite, alginate, kaolin, peat moss, wheat bran, oat bran.

scholarly journals Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 239
Author(s):  
Ida Szmigiel ◽  
Dorota Kwiatkowska ◽  
Marcin Łukaszewicz ◽  
Anna Krasowska
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Cell Walls ◽  
Cell Wall Composition ◽  
Subtilis Strain ◽  
Efficient Production ◽  
Plant Cell Walls ◽  
Rapeseed Cake ◽  
Wide Range ◽  
Agro Residues

Hemicellulose is the second most abundant plant heterogenous biopolymer. Among products obtained from a wide range of agro-residues, biosurfactants, e.g., surfactin (SU), are gaining increasing interest. Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake. This work aimed to investigate plant hemicellulose components as substrates promoting SU’s efficient production by B. subtilis 87Y. Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.

Endophytic Bacillus subtilis TR21 Improves Banana Plant Resistance to Fusarium oxysporum f. sp. cubense and Promotes Root Growth by Upregulating the Jasmonate and Brassinosteroid Biosynthesis Pathways

2021 ◽  
Author(s):  
Yunhao Sun ◽  
Bingzhi Huang ◽  
Ping Cheng ◽  
Chunji Li ◽  
Yanhong Chen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Root Growth ◽  
Fusarium Oxysporum ◽  
Plant Resistance ◽  
Growth Period ◽  
Subtilis Strain ◽  
Banana Plant ◽  
Musa Spp ◽  
Transcriptional Reprogramming ◽  
Race 1

The banana (Musa spp.) industry experiences dramatic annual losses from Fusarium wilt of banana (FWB) disease, which is caused by the fungus Fusarium oxysporum f. sp. cubense (FOC). Pisang Awak banana Fenza No. 1 (Musa spp. cv. Fenza No. 1), a major banana cultivar with high resistance to FOC race 4, is considered to be ideal for growth in problematic areas. However, Fenza No. 1 is still affected by FOC race 1 in the field. TR21 is an endophytic Bacillus subtilis strain isolated from orchids (Dendrobium sp.). Axillary spraying of banana plants with TR21 controls FWB, decreasing the growth period and increasing yields in the field. In this study, we established that TR21 increases root growth in different monocotyledonous plant species. By axillary inoculation, TR21 induced a similar transcriptomic change as that induced by FOC race 1 but also upregulated the biosynthetic pathways for the phytohormones brassinosteroid and jasmonate in Fenza No. 1 root tissues, indicating that TR21 increases FWB resistance, shortens growth period, and increases yield of banana by inducing specific transcriptional reprogramming and modulating phytohormone levels. These findings will contribute to the identification of candidate genes related to plant resistance against fungi in a non-model system and facilitate further study and exploitation of endophytic biocontrol agents.

scholarly journals Cell Walls of a Teichoic Acid Deficient Mutant of Bacillus subtilis

1971 ◽  
Vol 20 (3) ◽  
pp. 442-450 ◽  
Author(s):  
Jean Heijenoort ◽  
Daniele Menjon ◽  
Bernard Flouret ◽  
Jekisiel Szulmajster ◽  
Jean Laporte ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cell Walls ◽  
Teichoic Acid ◽  
Deficient Mutant

Effects of air discharge on surface charges and cell walls of Fusarium oxysporum

2021 ◽  
Author(s):  
Mengdie Liu ◽  
Hui Tang ◽  
Huiwen Jiang ◽  
Jie Li ◽  
Shoulei Yan ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Cell Walls ◽  
Surface Charges ◽  
Air Discharge

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Sign in / Sign up

Export Citation Format

Share Document