scholarly journals Plant responses to fungal volatiles involve global posttranslational thiol redox proteome changes that affect photosynthesis

2019 ◽  
Vol 42 (9) ◽  
pp. 2627-2644 ◽  
Author(s):  
Kinia Ameztoy ◽  
Marouane Baslam ◽  
Ángela María Sánchez‐López ◽  
Francisco José Muñoz ◽  
Abdellatif Bahaji ◽  
...  
Keyword(s):  
Plant Responses ◽  
Fungal Volatiles ◽  
Thiol Redox ◽  
Proteome Changes

scholarly journals Proteostatic Regulation of MEP and Shikimate Pathways by Redox-Activated Photosynthesis Signaling in Plants Exposed to Small Fungal Volatiles

2021 ◽  
Vol 12 ◽  
Author(s):  
Kinia Ameztoy ◽  
Ángela María Sánchez-López ◽  
Francisco José Muñoz ◽  
Abdellatif Bahaji ◽  
Goizeder Almagro ◽  
...  
Keyword(s):  
Protein Quality ◽  
Growth Promotion ◽  
Shikimate Pathway ◽  
Plant Responses ◽  
Clp Protease ◽  
Volatile Emissions ◽  
Molecular Masses ◽  
Promote Plant Growth ◽  
Thiol Redox ◽  
Protein Quality Control System

Microorganisms produce volatile compounds (VCs) with molecular masses of less than 300 Da that promote plant growth and photosynthesis. Recently, we have shown that small VCs of less than 45 Da other than CO2 are major determinants of plant responses to fungal volatile emissions. However, the regulatory mechanisms involved in the plants’ responses to small microbial VCs remain unclear. In Arabidopsis thaliana plants exposed to small fungal VCs, growth promotion is accompanied by reduction of the thiol redox of Calvin-Benson cycle (CBC) enzymes and changes in the levels of shikimate and 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway-related compounds. We hypothesized that plants’ responses to small microbial VCs involve post-translational modulation of enzymes of the MEP and shikimate pathways via mechanisms involving redox-activated photosynthesis signaling. To test this hypothesis, we compared the responses of wild-type (WT) plants and a cfbp1 mutant defective in a redox-regulated isoform of the CBC enzyme fructose-1,6-bisphosphatase to small VCs emitted by the fungal phytopathogen Alternaria alternata. Fungal VC-promoted growth and photosynthesis, as well as metabolic and proteomic changes, were substantially weaker in cfbp1 plants than in WT plants. In WT plants, but not in cfbp1 plants, small fungal VCs reduced the levels of both transcripts and proteins of the stromal Clp protease system and enhanced those of plastidial chaperonins and co-chaperonins. Consistently, small fungal VCs promoted the accumulation of putative Clp protease clients including MEP and shikimate pathway enzymes. clpr1-2 and clpc1 mutants with disrupted plastidial protein homeostasis responded weakly to small fungal VCs, strongly indicating that plant responses to microbial volatile emissions require a finely regulated plastidial protein quality control system. Our findings provide strong evidence that plant responses to fungal VCs involve chloroplast-to-nucleus retrograde signaling of redox-activated photosynthesis leading to proteostatic regulation of the MEP and shikimate pathways.

scholarly journals Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry

Proteomes ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 10
Author(s):  
Bo Yang ◽  
Andrew Thomas ◽  
C. Greenlief
Keyword(s):  
Molecular Mechanisms ◽  
Redox Homeostasis ◽  
Principal Component ◽  
Fertilizer Treatment ◽  
Plant Responses ◽  
Two Dimensional Gel Electrophoresis ◽  
Critical Pathways ◽  
Specialty Crop ◽  
N Treatment ◽  
Proteome Changes

American elderberry (Sambucus nigra subsp. canadensis) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elder in response to varying levels of N-treatment remain undefined. To reveal plant responses to N, comparative proteomic analyses were performed to determine consistent changes in three genotypes of elderberry leaves (Adams II, Bob Gordon and Wyldewood) grown under different N-fertilizer treatments. 165 proteins separated by two dimensional gel electrophoresis showed significant differences in abundance (p < 0.05 and greater than 2-fold). Principal component analysis of the abundance profiles of these proteins revealed Bob Gordon as a distinct genotype. The 165 proteins were identified by mass spectrometry and showed similar functional distributions in these genotypes underlying the N-treatment. Among the proteins identified, 23 are mainly involved in photosynthesis, protein metabolism and redox homeostasis. Their abundance profiles were not altered upon exposure to N or genotype. These results provide novel insights into plant responses to fertilizer treatment at the proteome level and could lead to a better understanding of molecular mechanisms of elderberry growth.

Temporal dynamics of mycorrhizal fungal communities and co-associations with grassland plant communities following experimental manipulation of rainfall

2019 ◽  
Author(s):  
Coline Deveautour ◽  
Sally Power ◽  
Kirk Barnett ◽  
Raul Ochoa-Hueso ◽  
Suzanne Donn ◽  
...  
Keyword(s):  
Community Composition ◽  
Plant Community ◽  
Plant Communities ◽  
Fungal Community ◽  
Mycorrhizal Fungi ◽  
Temporal Dynamics ◽  
Arbuscular Mycorrhizal ◽  
Fungal Communities ◽  
Plant Responses ◽  
Rainfall Regimes

Climate models project overall a reduction in rainfall amounts and shifts in the timing of rainfall events in mid-latitudes and sub-tropical dry regions, which threatens the productivity and diversity of grasslands. Arbuscular mycorrhizal fungi may help plants to cope with expected changes but may also be impacted by changing rainfall, either via the direct effects of low soil moisture on survival and function or indirectly via changes in the plant community. In an Australian mesic grassland (former pasture) system, we characterised plant and arbuscular mycorrhizal (AM) fungal communities every six months for nearly four years to two altered rainfall regimes: i) ambient, ii) rainfall reduced by 50% relative to ambient over the entire year and iii) total summer rainfall exclusion. Using Illumina sequencing, we assessed the response of AM fungal communities sampled from contrasting rainfall treatments and evaluated whether variation in AM fungal communities was associated with variation in plant community richness and composition. We found that rainfall reduction influenced the fungal communities, with the nature of the response depending on the type of manipulation, but that consistent results were only observed after more than two years of rainfall manipulation. We observed significant co-associations between plant and AM fungal communities on multiple dates. Predictive co-correspondence analyses indicated more support for the hypothesis that fungal community composition influenced plant community composition than vice versa. However, we found no evidence that altered rainfall regimes were leading to distinct co-associations between plants and AM fungi. Overall, our results provide evidence that grassland plant communities are intricately tied to variation in AM fungal communities. However, in this system, plant responses to climate change may not be directly related to impacts of altered rainfall regimes on AM fungal communities. Our study shows that AM fungal communities respond to changes in rainfall but that this effect was not immediate. The AM fungal community may influence the composition of the plant community. However, our results suggest that plant responses to altered rainfall regimes at our site may not be resulting via changes in the AM fungal communities.

Plastic Mulch Color Effects on Light Micro-environment and Watermelon Plant Growth

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 474d-474
Author(s):  
N.K. Damayanthi Ranwala ◽  
Dennis R. Decoteau
Keyword(s):  
Plant Growth ◽  
Light Transmission ◽  
Root Zone ◽  
Dry Mass ◽  
Plant Responses ◽  
Plastic Mulch ◽  
Plant Parts ◽  
Reflected Light ◽  
Total Light ◽  
Dry Mass Partitioning

This study was conducted to evaluate the spectral properties of various colored plastic color mulches and to determine the effects of upwardly reflected light from the mulch surfaces on watermelon plant growth when differences in root zone temperatures are minimized. Two-week-old watermelon plants were grown with black mulch, red-painted mulch, SRM-Red mulch (Sonoco, Inc., Harstville, S.C.), and white mulch. Total light reflection (58 μmol·m–2·s–1 in 400–700 nm) and red: far-red (R:FR = 0.44) of reflected light were lower in black mulch and highest in white mulch (634 and 0.92, respectively). Both black mulch and white mulch had same blue:red (B:R = 0.6) while white mulch had higher B:FR (0.58) in reflected light compared to black mulch (0.26). Reflective properties of red mulches were somewhat similar, and R:FR, B:R, and B:FR were 0.8, 0.2, and 0.18, respectively. However, SRM-Red mulch had highest total light (355 μmol·m–2·s–1 in 400–700 nm) transmission through the mulch, and R:FR, B:R, and B:FR were 0.84, 0.28, and 0.23, respectively. Light transmission through the other mulches was nonsignificant. Watermelon plants grown with black mulch and red mulches had higher internode lengths compared to white mulch after 20 days. Further, plants grown under black had significant higher petiole elongation accompanied with higher dry mass partitioning to petioles, and lower partitioning to roots, stems, and leaves. There was no effects of surface mulch color on total plant dry mass or photosynthesis although plants with black had higher transpiration rate. This suggests the differential regulation of dry mass partitioning among plant parts due to mulch color. The similar plant responses with black mulch and white mulch to plants treated with FR or R light at the end of photoperiod implies the involvement of phytochrome regulation of growth due to mulch surface color.

Novel Scaffolds for Leishmania infantum Trypanothione Reductase Inhibitors Derived from Brazilian Natural Products Biodiversity

2021 ◽  
Vol 18 (4) ◽  
pp. 398-418
Author(s):  
Vinícius Guimarães da Paixão ◽  
Samuel Silva da Rocha Pita
Keyword(s):  
Natural Products ◽  
Leishmania Infantum ◽  
In Vitro Assays ◽  
Current Therapy ◽  
Pharmacokinetic Analysis ◽  
Trypanothione Reductase ◽  
Resistant Species ◽  
Reductase Inhibitors ◽  
Thiol Redox

Background: Leishmania infantum causes the most lethal form of Leishmaniasis: Visceral leishmaniasis. Current therapy for this disease is related to the development of drug-resistant species and toxicity. Trypanothione Reductase (LiTR), a validated target for the drug discovery process, is involved with parasites' thiol-redox metabolism. Objective: In this study, through Virtual Screening employing two distinct Natural Products Brazilian databases, we aimed to identify novel inhibitor scaffolds against LiTR. Results: Thus, the “top 10” LiTR-ligand energies have been selected and their interaction profiles into LiTR sites through the AuPosSOM server have been verified. Finally, Pred-hERG, Aggregator Advisor, FAF-DRUGS, pkCSM and DataWarrior were employed and their results allowed us to evaluate, respectively, the cardiotoxicity, aggregation capacity, presence of false-positive compounds (PAINS) and their toxicities. Conclusion: Three molecules that overcame the in silico pharmacokinetic analysis and have a good interaction with LiTR, were chosen to use in vitro assays hoping that our computational results reported here would aid the development of new anti-leishmanial compounds.

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