scholarly journals Helper component-proteinase enhances the activity of 1-deoxy-D-xylulose-5-phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y-infected tobacco

2015 ◽  
Vol 38 (10) ◽  
pp. 2023-2034 ◽  
Author(s):  
Heng Li ◽  
Dongyuan Ma ◽  
Yongsheng Jin ◽  
Yayi Tu ◽  
Liping Liu ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Helper Component ◽  
Helper Component Proteinase ◽  
Infected Tobacco ◽  
Phosphate Synthase

scholarly journals The Helper Component Proteinase Cistron of Potato virus Y Induces Hypersensitivity and Resistance in Potato Genotypes Carrying Dominant Resistance Genes on Chromosome IV

2011 ◽  
Vol 24 (7) ◽  
pp. 787-797 ◽  
Author(s):  
Benoît Moury ◽  
Bernard Caromel ◽  
Elisabeth Johansen ◽  
Vincent Simon ◽  
Laura Chauvin ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Dominant Gene ◽  
Recombination Breakpoint ◽  
Nucleotide Substitutions ◽  
Helper Component ◽  
Systemic Movement ◽  
Recent Emergence ◽  
Clade C ◽  
Helper Component Proteinase

The Nctbr and Nytbr genes in Solanum tuberosum determine hypersensitive reactions, characterized by necrotic reactions and restriction of the virus systemic movement, toward isolates belonging to clade C and clade O of Potato virus Y (PVY), respectively. We describe a new resistance from S. sparsipilum which possesses the same phenotype and specificity as Nctbr and is controlled by a dominant gene designated Ncspl. Ncspl maps on potato chromosome IV close or allelic to Nytbr. The helper component proteinase (HC-Pro) cistron of PVY was shown to control necrotic reactions and resistance elicitation in plants carrying Ncspl, Nctbr, and Nytbr. However, inductions of necrosis and of resistance to the systemic virus movement in plants carrying Ncspl reside in different regions of the HC-Pro cistron. Also, genomic determinants outside the HC-Pro cistron are involved in the systemic movement of PVY after induction of necroses on inoculated leaves of plants carrying Nytbr. These results suggest that the Nytbr resistance may have been involved in the recent emergence of PVY isolates with a recombination breakpoint near the junction of HC-Pro and P3 cistrons in potato crops. Therefore, this emergence could constitute one of the rare examples of resistance breakdown by a virus which was caused by recombination instead of by successive accumulation of nucleotide substitutions.

scholarly journals Mutations in the Region Encoding the Central Domain of Helper Component-Proteinase (HC-Pro) Eliminate Potato Virus X/Potyviral Synergism

Virology ◽  
1997 ◽  
Vol 231 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Xing Ming Shi ◽  
Heather Miller ◽  
Jeanmarie Verchot ◽  
James C. Carrington ◽  
Vicki Bowman Vance
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Central Domain ◽  
Helper Component ◽  
Helper Component Proteinase

SOME EFFECTS OF FORMALDEHYDE ON POTATO VIRUS Y IN VITRO, AND ABILITY OF APHIDS TO TRANSMIT THE VIRUS WHEN THEIR STYLETS ARE TREATED WITH FORMALDEHYDE

1955 ◽  
Vol 1 (9) ◽  
pp. 783-793 ◽  
Author(s):  
R. H. E. Bradley ◽  
R. Y. Ganong
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Tobacco Plants ◽  
Considerable Distance ◽  
Formaldehyde Treatment ◽  
Aphid Vectors ◽  
Infected Tobacco

Potato virus Y (PVY) was made noninfective by incubation with formaldehyde in vitro. Yet this noninfective virus reacted with PVY antiserum and caused antibodies to be produced in rabbits as readily as infective PVY. A method is described for baring the stylets of living aphids beyond the end of the labium, which normally encloses the stylets. Specimens of Myzus persicae (Sulz.) infective with PVY were made noninfective by treating the stylets for 30 sec. with concentrations of formaldehyde as low as 0.03%; and 0.25% formaldehyde caused the same effect in five seconds. Aphids were also made noninfective when the proboscis with the tip of the stylets bared was treated with formaldehyde, even after the stylets had been inserted a considerable distance into infected tobacco plants. By contrast, aphids usually remained infective when the proboscis with the stylets enclosed in the labium was treated with similar concentrations of formaldehyde. However, formaldehyde treatment of the stylets did not affect the ability of aphids immediately thereafter to acquire and transmit PVY. These results are compatible with the hypothesis that viruses transmitted like PVY are carried by the stylets of their aphid vectors.

scholarly journals Effect of mutations within the Cys-rich region of potyvirus helper component-proteinase on self-interaction

1999 ◽  
Vol 80 (11) ◽  
pp. 2809-2812 ◽  
Author(s):  
Silvio Urcuqui-Inchima ◽  
Ivan G. Maia ◽  
Gabrièle Drugeon ◽  
Anne-Lise Haenni ◽  
Françoise Bernardi
Keyword(s):  
Amino Acids ◽  
Potato Virus Y ◽  
Yeast Two Hybrid ◽  
Rich Region ◽  
Conserved Residues ◽  
Helper Component ◽  
Yeast Two Hybrid System ◽  
Terminal Amino ◽  
Helper Component Proteinase ◽  
Two Hybrid

The first ∼60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment.

Expression of the “helper component” protein of potato virus Y (PVY) inE. coli: Possible involvement of a third protease

Virus Genes ◽  
1993 ◽  
Vol 7 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Yehuda Stram ◽  
Aviva Chetsrony ◽  
Hagai Karchi ◽  
Miri Karchi ◽  
Orit Edelbaum ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Helper Component ◽  
Component Protein
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