scholarly journals The alternative respiratory pathway mediates carboxylate synthesis in white lupin cluster roots under phosphorus deprivation

2013 ◽  
Vol 37 (4) ◽  
pp. 922-928 ◽  
Author(s):  
IGOR FLOREZ-SARASA ◽  
HANS LAMBERS ◽  
XING WANG ◽  
PATRICK M. FINNEGAN ◽  
MIQUEL RIBAS-CARBO
Keyword(s):  
White Lupin ◽  
Cluster Roots ◽  
Respiratory Pathway ◽  
Alternative Respiratory Pathway ◽  
Phosphorus Deprivation

scholarly journals Nodulated White Lupin Plants Growing in Contaminated Soils Accumulate Unusually High Mercury Concentrations in Their Nodules, Roots and Especially Cluster Roots

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 302
Author(s):  
Miguel A. Quiñones ◽  
Susana Fajardo ◽  
Mercedes Fernández-Pascual ◽  
M. Mercedes Lucas ◽  
José J. Pueyo
Keyword(s):  
Contaminated Soils ◽  
Lupinus Albus ◽  
White Lupin ◽  
Cluster Roots ◽  
Cluster Root ◽  
Bioaccumulation Factors ◽  
Aerial Parts ◽  
High Mercury ◽  
Lupinus Albus L ◽  
Hg Accumulation

Two white lupin (Lupinus albus L.) cultivars were tested for their capacity to accumulate mercury when grown in Hg-contaminated soils. Plants inoculated with a Bradyrhizobium canariense Hg-tolerant strain or non-inoculated were grown in two highly Hg-contaminated soils. All plants were nodulated and presented a large number of cluster roots. They accumulated up to 600 μg Hg g−1 DW in nodules, 1400 μg Hg g−1 DW in roots and 2550 μg Hg g−1 DW in cluster roots. Soil, and not cultivar or inoculation, was accountable for statistically significant differences. No Hg translocation to leaves or seeds took place. Inoculated L. albus cv. G1 plants were grown hydroponically under cluster root-promoting conditions in the presence of Hg. They accumulated about 500 μg Hg g−1 DW in nodules and roots and up to 1300 μg Hg g−1 DW in cluster roots. No translocation to the aerial parts occurred. Bioaccumulation factors were also extremely high, especially in soils and particularly in cluster roots. To our knowledge, Hg accumulation in cluster roots has not been reported to date. Our results suggest that inoculated white lupin might represent a powerful phytoremediation tool through rhizosequestration of Hg in contaminated soils. Potential uptake and immobilization mechanisms are discussed.

scholarly journals Update on White Lupin Cluster Root Acclimation to Phosphorus Deficiency Update on Lupin Cluster Roots

2011 ◽  
Vol 156 (3) ◽  
pp. 1025-1032 ◽  
Author(s):  
Lingyun Cheng ◽  
Bruna Bucciarelli ◽  
Jianbo Shen ◽  
Deborah Allan ◽  
Carroll P. Vance
Keyword(s):  
Phosphorus Deficiency ◽  
White Lupin ◽  
Cluster Roots ◽  
Cluster Root

Cloning and Analysis of the Alternative Oxidase Gene of Neurospora crassa

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 129-140 ◽  
Author(s):  
Qiuhong Li ◽  
R Gary Ritzel ◽  
Lesley L T McLean ◽  
Lee McIntosh ◽  
Tak Ko ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Alternative Oxidase ◽  
Electron Flow ◽  
Mitochondrial Protein ◽  
Transcriptional Level ◽  
Wild Type ◽  
Respiratory Pathway ◽  
Oxidase Gene ◽  
Alternative Respiratory Pathway ◽  
Cytochrome Pathway

Mitochondria of Neurospora crassa contain a cyanide-resistant alternative respiratory pathway in addition to the cytochrome pathway. The alternative oxidase is present only when electron flow through the cytochrome chain is restricted. Both genomic and cDNA copies for the alternative oxidase gene have been isolated and analyzed. The sequence of the predicted protein is homologous to that of other species. The mRNA for the alternative oxidase is scarce in wild-type cultures grown under normal conditions, but it is abundant in cultures grown in the presence of chloramphenicol, an inhibitor of mitochondrial protein synthesis, or in mutants deficient in mitochondrial cytochromes. Thus, induction of alternative oxidase appears to be at the transcriptional level. Restriction fragment length polymorphism mapping of the isolated gene demonstrated that it is located in a position corresponding to the aod-1 locus. Sequence analysis of mutant aod-1 alleles reveals mutations affecting the coding sequence of the alternative oxidase. The level of aod-1 mRNA in an aod-2 mutant strain that had been grown in the presence of chloramphenicol was reduced several fold relative to wild-type, supporting the hypothesis that the product of aod-2 is required for optimal expression of aod-1.

Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis

2019 ◽  
Vol 70 (20) ◽  
pp. 5575-5590 ◽  
Author(s):  
Shan-Shan Wei ◽  
Wei-Tao Niu ◽  
Xiao-Ting Zhai ◽  
Wei-Qian Liang ◽  
Meng Xu ◽  
...  
Keyword(s):  
Alternative Oxidase ◽  
Redox Homeostasis ◽  
Superoxide Dismutase 1 ◽  
Respiratory Pathway ◽  
Cellular Processes ◽  
Growth Defects ◽  
Alternative Respiratory Pathway ◽  
Abnormal Mitochondria ◽  
Shock Proteins ◽  
Severe Growth

Abstract The 70 kDa heat shock proteins function as molecular chaperones and are involved in diverse cellular processes. However, the functions of the plant mitochondrial HSP70s (mtHSC70s) remain unclear. Severe growth defects were observed in the Arabidopsis thaliana mtHSC70-1 knockout lines, mthsc70-1a and mthsc70-1b. Conversely, the introduction of the mtHSC70-1 gene into the mthsc70-1a background fully reversed the phenotypes, indicating that mtHSC70-1 is essential for plant growth. The loss of mtHSC70-1 functions resulted in abnormal mitochondria and alterations to respiration because of an inhibition of the cytochrome c oxidase (COX) pathway and the activation of the alternative respiratory pathway. Defects in COX assembly were observed in the mtHSC70-1 knockout lines, leading to decreased COX activity. The mtHSC70-1 knockout plants have increased levels of reactive oxygen species (ROS). The introduction of the Mn-superoxide dismutase 1 (MSD1) or the catalase 1 (CAT1) gene into the mthsc70-1a plants decreased ROS levels, reduced the expression of alternative oxidase, and partially rescued growth. Taken together, our data suggest that mtHSC70-1 plays important roles in the establishment of COX-dependent respiration.

scholarly journals Expression of plasma membrane H+ -ATPase in cluster roots of white lupin under phosphorus deficiency

2019 ◽  
Vol 182 (6) ◽  
pp. 867-870
Author(s):  
Sophie Stein ◽  
Franziska Faust ◽  
Stephan Jung ◽  
Sven Schubert
Keyword(s):  
Plasma Membrane ◽  
Phosphorus Deficiency ◽  
White Lupin ◽  
Cluster Roots

The Role of Cu in Respiration of Pea Plants and Heterotrophically Growing Scenedesmus Cells

1988 ◽  
Vol 43 (5-6) ◽  
pp. 438-442 ◽  
Author(s):  
Matilde Barón Ayala ◽  
Gerhard Sandmann
Keyword(s):  
Cytochrome Oxidase ◽  
Respiratory Activity ◽  
The Other ◽  
Nadh Oxidation ◽  
Alternative Respiration ◽  
Respiratory Pathway ◽  
Cu Deficiency ◽  
Alternative Respiratory Pathway

In Scenedesmus about half of NADH oxidation proceeds via a cyanide-sensitive and the other half via a cyanide-insensitive respiratory pathway. In contrast, respiration is completely cyanide sensitive in pea indicating that the alternative respiratory pathway is absent. Cu deficiency in pea plants and in heterotrophically grown Scenedesmus cells interferes with respiratory activity of mitochondria. In both organisms, the cyanide-sensitive NADH oxidation was strongly decreased during cultivation in low Cu media. Cu sensitivity was also observed for the alternative respiratory pathway in Scenedesmus. These results suggest that a Cu-containing component is involved in the alternative respiratory pathway. This is the main reason why alternative respiration cannot be regarded as a compensation for low cytochrome-oxidase activities during Cu starvation. The Cu dependency of the cyanide-sensitive respiration was located at the site of cytochrome oxidase. A strong coordination of the biosynthesis of the Cu-containing cytochrome-oxidase complex was evident. When the endogenous Cu pool was low, formation of cytochrome aa3, another component of cytochrome oxidase, was also decreased.

scholarly journals Alternative Respiratory Pathway

1980 ◽  
Vol 65 (4) ◽  
pp. 669-674 ◽  
Author(s):  
James N. Siedow ◽  
Mark E. Girvin
Keyword(s):  
Respiratory Pathway ◽  
Alternative Respiratory Pathway
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