scholarly journals Perspective and application of molecular markers linked to the cytoplasm types and male‐fertility restorer locus in onion ( Allium cepa )

2021 ◽  
Author(s):  
Dalasanuru Chandregowda Manjunathagowda
Keyword(s):  
Molecular Markers ◽  
Allium Cepa ◽  
Male Fertility ◽  
Fertility Restorer

Molecular markers in identification of sunflower pollen fertility restorer genes

2009 ◽  
Vol 35 (6) ◽  
pp. 367-370 ◽  
Author(s):  
I. N. Anisimova ◽  
V. A. Gavrilova ◽  
V. T. Rozhkova ◽  
G. I. Timofeeva ◽  
M. A. Tikhonova
Keyword(s):  
Molecular Markers ◽  
Pollen Fertility ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Pollen Fertility Restorer ◽  
Fertility Restorer Genes

scholarly journals Identification of Ms2, a Novel Locus Controlling Male-fertility Restoration of Cytoplasmic Male-sterility in Onion (Allium Cepa L.), and Development of Tightly Linked Molecular Markers

2021 ◽  
Author(s):  
Nari Yu ◽  
Sunggil Kim
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Allium Cepa ◽  
Male Fertility ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
Chromosome 2 ◽  
Rna Seq ◽  
Male Sterile ◽  
Male Fertility Restoration

Abstract Cytoplasmic male-sterility (CMS) has been exclusively used to produce F1 hybrid seeds of onion (Allium cepa L.). A single nuclear locus, Ms, is known to restore male-fertility of CMS in onions. Unstable male-sterile onions producing a small amount of pollen grains have been identified in a previous study. When such unstable male-sterile onions were crossed with stable male-sterile onions containing CMS-T cytoplasm, male-fertility was completely restored, although genotypes of the Ms locus were homozygous recessive. Inheritance patterns indicated that male-fertility restoration was controlled by a single locus designated as Ms2. A combined approach of bulked segregant analysis and RNA-seq was used to identify candidate genes for the Ms2 locus. High resolution melting (HRM) markers were developed based on single nucleotide polymorphisms (SNPs) detected by RNA-Seq. Comparative mapping of the Ms2 locus showed that Ms2 was positioned at the end of chromosome 2 with a distance of approximately 70 cM away from the Ms locus. Although 38 contigs containing reliable SNPs were analyzed using recombinants selected from 1,344 individuals, no contig showed perfect linkage to Ms2. Interestingly, transcription levels of orf725, a CMS-associated gene in onions, were significantly reduced in male-fertile individuals of segregating populations. However, no significant change in its transcription level was observed in individuals of a segregating population with male-fertility phenotypes determined by the Ms locus, suggesting that male-fertility restoration mechanism of Ms2 might be different from that of the Ms locus.

Validation of molecular markers linked to fertility restorer gene(s) for WA-CMS lines of rice

Euphytica ◽  
2009 ◽  
Vol 167 (2) ◽  
pp. 217-227 ◽  
Author(s):  
N. K. Sheeba ◽  
B. C. Viraktamath ◽  
S. Sivaramakrishnan ◽  
M. G. Gangashetti ◽  
Pawan Khera ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene
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