Cloning and characterization of a putative orthologue of the wheat vernalization ( VRN1 ) gene in perennial wheatgrass ( Agropyron cristatum )

2020 ◽  
Vol 139 (6) ◽  
pp. 1290-1298
Author(s):  
Adoración Cabrera ◽  
Alejandro Copete‐Parada ◽  
Eva Madrid
Keyword(s):  
Agropyron Cristatum ◽  
Vrn1 Gene

scholarly journals Development and Characterization of Wheat-Agropyron cristatum Introgression Lines Induced by Gametocidal Genes and Wheat ph1b Mutant

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 277
Author(s):  
Alejandro Copete-Parada ◽  
Carmen Palomino ◽  
Adoración Cabrera
Keyword(s):  
Genetic Resources ◽  
Agropyron Cristatum ◽  
Homologous Gene ◽  
Aegilops Cylindrica ◽  
Induction Frequency ◽  
Structural Aberrations ◽  
Translocation Lines ◽  
New Germplasm

The P genome of Agropyron cristatum Gaertn. contains many desirable genes that can be utilized as genetic resources to improve wheat. In this research, we used both the gametocidal chromosome 2Cc and the pairing homologous gene (Ph1b) mutant to induce structural aberrations and translocations between wheat and the 4P, 5P, and 6P genome chromosomes. By using the two approaches, a total of 19 wheat-A. cristatum translocations have been identified, in which 13 were induced by the Triticum aestivum cv. Chinese Spring (CS) ph1b mutant (CS ph1b) and six were induced by gametocidal chromosome 2Cc from Aegilops cylindrica Host. The wheat-4P, -5P and -6P A. cristatum translocations were characterized by in situ hybridization and by a set of conserved orthologous set (COS) molecular markers. The aberrations included centromeric translocations, terminal translocations, dicentric translocations, and deletions. The average induction frequency of chromosome structural aberrations was 10.9% using gametocidal 2Cc chromosome and 8.8% using ph1b mutant. The highest frequency obtained was for chromosome 4P using both approaches. All the wheat-A. cristatum translocation lines obtained were valuable for identifying A. cristatum chromosome 4P, 5P, and 6P related genes. In addition, these lines provided genetic resources and new germplasm accessions for the genetic improvement of wheat.

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

Euphytica ◽  
2014 ◽  
Vol 201 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Virginia Ochoa ◽  
Eva Madrid ◽  
Mahmoud Said ◽  
Diego Rubiales ◽  
Adoración Cabrera
Keyword(s):  
Leaf Rust ◽  
Common Wheat ◽  
Chromosome Translocation ◽  
Agropyron Cristatum ◽  
Cytogenetic Characterization

Development and characterization of novel Triticum aestivum-Agropyron cristatum 6P Robertsonian translocation lines

2021 ◽  
Vol 41 (10) ◽  
Author(s):  
Kai Qi ◽  
Haiming Han ◽  
Jinpeng Zhang ◽  
Shenghui Zhou ◽  
Xiuquan Li ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Robertsonian Translocation ◽  
Agropyron Cristatum ◽  
Translocation Lines

Characterization of a Novel Wheat-Agropyron cristatum 2P Disomic Addition Line with Powdery Mildew Resistance

Crop Science ◽  
2016 ◽  
Vol 56 (5) ◽  
pp. 2390-2400 ◽  
Author(s):  
Qingfeng Li ◽  
Yuqing Lu ◽  
Cuili Pan ◽  
Jinpeng Zhang ◽  
Weihua Liu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Agropyron Cristatum ◽  
Addition Line ◽  
Mildew Resistance ◽  
Disomic Addition Line

scholarly journals Genotyping-by-Sequencing Enhances Genetic Diversity Analysis of Crested Wheatgrass [Agropyron cristatum (L.) Gaertn.]

2018 ◽  
Vol 19 (9) ◽  
pp. 2587 ◽  
Author(s):  
Kiran Baral ◽  
Bruce Coulman ◽  
Bill Biligetu ◽  
Yong-Bi Fu
Keyword(s):  
Genetic Diversity ◽  
Bayesian Analysis ◽  
Genotyping By Sequencing ◽  
Agropyron Cristatum ◽  
Grass Species ◽  
Diversity Analysis ◽  
Principal Coordinates Analysis ◽  
Crested Wheatgrass ◽  
Principal Coordinates

Molecular characterization of unsequenced plant species with complex genomes is now possible by genotyping-by-sequencing (GBS) using recent next generation sequencing technologies. This study represents the first use of GBS application to sample genome-wide variants of crested wheatgrass [Agropyron cristatum (L.) Gaertn.] and assess the genetic diversity present in 192 genotypes from 12 tetraploid lines. Bioinformatic analysis identified 45,507 single nucleotide polymorphism (SNP) markers in this outcrossing grass species. The model-based Bayesian analysis revealed four major clusters of the samples assayed. The diversity analysis revealed 15.8% of SNP variation residing among the 12 lines, and 12.1% SNP variation present among four genetic clusters identified by the Bayesian analysis. The principal coordinates analysis and dendrogram were able to distinguish four lines of Asian origin from Canadian cultivars and breeding lines. These results serve as a valuable resource for understanding genetic variability, and will aid in the genetic improvement of this outcrossing polyploid grass species for forage production. These findings illustrate the potential of GBS application in the characterization of non-model polyploid plants with complex genomes.

Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of Agropyron cristatum

Euphytica ◽  
2009 ◽  
Vol 172 (3) ◽  
pp. 363-372 ◽  
Author(s):  
M. Wu ◽  
J. P. Zhang ◽  
J. C. Wang ◽  
X. M. Yang ◽  
A. N. Gao ◽  
...  
Keyword(s):  
Repetitive Sequences ◽  
Agropyron Cristatum ◽  
Scar Markers

Characterization of a world collection of Agropyron cristatum accessions

2018 ◽  
Vol 65 (5) ◽  
pp. 1455-1469 ◽  
Author(s):  
Alejandro Copete ◽  
Roberto Moreno ◽  
Adoración Cabrera
Keyword(s):  
Agropyron Cristatum ◽  
World Collection

On the use of SSR markers for the genetic characterization of the Agropyron cristatum (L.) Gaertn. in Northern China

2007 ◽  
Vol 55 (3) ◽  
pp. 389-396 ◽  
Author(s):  
Y. H. Che ◽  
H. J. Li ◽  
Y. P. Yang ◽  
X. M. Yang ◽  
X. Q. Li ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Characterization ◽  
Northern China ◽  
Agropyron Cristatum

Chromosomal location and molecular characterization of three grain hardness genes in Agropyron cristatum

Euphytica ◽  
2019 ◽  
Vol 215 (10) ◽  
Author(s):  
Adoración Cabrera ◽  
Laura Castellano ◽  
Rocío Recio ◽  
Juan B. Alvarez
Keyword(s):  
Molecular Characterization ◽  
Chromosomal Location ◽  
Agropyron Cristatum ◽  
Grain Hardness
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