Development of a kompetitive allele-specific PCR marker for selection of the mutatedWx-D1dallele in wheat breeding

2017 ◽  
Vol 136 (4) ◽  
pp. 460-466 ◽  
Author(s):  
Xin Yi ◽  
Zhengning Jiang ◽  
Wenjing Hu ◽  
Yun Zhao ◽  
Tongde Bie ◽  
...  
Keyword(s):  
Wheat Breeding ◽  
Pcr Marker ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Selection Of

Isolation of a gibberellin-insensitive dwarfing gene, Rht-B1e, and development of an allele-specific PCR marker

2012 ◽  
Vol 30 (3) ◽  
pp. 1443-1451 ◽  
Author(s):  
Aixia Li ◽  
Wenlong Yang ◽  
Xiaoli Guo ◽  
Dongcheng Liu ◽  
Jiazhu Sun ◽  
...  
Keyword(s):  
Pcr Marker ◽  
Dwarfing Gene ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Isolation of the dwarfing Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker

2015 ◽  
Vol 35 (11) ◽  
Author(s):  
M. S. Bazhenov ◽  
M. G. Divashuk ◽  
Y. Amagai ◽  
N. Watanabe ◽  
G. I. Karlov
Keyword(s):  
Pcr Marker ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Chromosome location and allele-specific PCR markers for marker-assisted selection of the oat crown rust resistance gene Pc91

2013 ◽  
Vol 32 (3) ◽  
pp. 679-686 ◽  
Author(s):  
B. N. Gnanesh ◽  
J. Mitchell Fetch ◽  
J. G. Menzies ◽  
A. D. Beattie ◽  
P. E. Eckstein ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Rust Resistance ◽  
Chromosome Location ◽  
Pcr Markers ◽  
Specific Pcr ◽  
Oat Crown Rust ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Selection Of

scholarly journals Mapping of Two New Rust Resistance Genes Uvf-2 and Uvf-3 in Faba Bean

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1370
Author(s):  
Usman Ijaz ◽  
Shimna Sudheesh ◽  
Sukhjiwan Kaur ◽  
Abdus Sadeque ◽  
Harbans Bariana ◽  
...  
Keyword(s):  
Faba Bean ◽  
Rust Resistance ◽  
Grain Legume ◽  
Sources Of Resistance ◽  
Specific Pcr ◽  
Rust Diseases ◽  
Eastern Australia ◽  
Allele Specific ◽  
Allele Specific Pcr ◽  
Selection Of

Faba bean is gaining attention in Australia as a rotation grain legume where most of the country’s produce is exported. Rust, caused by Uromyces viciae-fabae, is a major constraint to Faba bean production in eastern Australia and its chemical control results in increased cost of production. The deployment of diverse sources of resistance in new cultivars underpins economic and eco-friendly control of rust diseases of crops. A selection from cultivar Doza (Doza#12034) and a European accession Ac1655 exhibited seedling rust resistance against U. viciae-fabae pathotype 24–40. Doza#12034 and Ac1655 were crossed with a susceptible genotype Fiord and recombinant inbred line (RIL) F6 populations were generated. Rust tests on Fiord/Doza#12034 and Fiord/Ac1655 F4 and F6 populations demonstrated monogenic inheritance of resistance in both crosses and the underlying resistance loci were named Uvf-2 and Uvf-3, respectively. Genetic mapping of both RIL populations located Uvf-2 and Uvf-3 in chromosomes III and V, respectively. The SNPs that showed linkage with Uvf-2 and Uvf-3 were converted into kompititive allele specific PCR (KASP) assays. Markers KASP_Vf_0703 and KASP_C250539 flanked Uvf-2 at 4.9 cM and 2.9 cM distances, whereas Uvf-3 was flanked by KASP_Ac×F165 (2.5 cM) and KASP_vf_1090 (10.1 cM). Markers KASP_Vf_0703 and KASP_Ac×F165 can be used for marker-assisted selection of Uvf-2 and Uvf-3, respectively, after confirming parental polymorphisms.

Simple and Rapid Detection of Factor V Leiden by Allele-specific PCR Amplification

1996 ◽  
Vol 75 (05) ◽  
pp. 757-759 ◽  
Author(s):  
Rainer Blasczyk ◽  
Markus Ritter ◽  
Christian Thiede ◽  
Jenny Wehling ◽  
Günter Hintz ◽  
...  
Keyword(s):  
Direct Sequencing ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Pcr Amplification ◽  
Factor V ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Allele Specific ◽  
Specific Amplification ◽  
Allele Specific Pcr

SummaryResistance to activated protein C is the most common hereditary cause for thrombosis and significantly linked to factor V Leiden. In this study, primers were designed to identify the factor V mutation by allele-specific PCR amplification. 126 patients with thromboembolic events were analysed using this technique, PCR-RFLP and direct sequencing. The concordance between these techniques was 100%. In 27 patients a heterozygous factor VGln506 mutation was detected, whereas one patient with recurrent thromboembolism was homozygous for the point mutation. Due to its time- and cost-saving features allele-specific amplification should be considered for screening of factor VGln506.

Sign in / Sign up

Export Citation Format

Share Document