Cytological observation of anther structure and genetic investigation of a new type of cytoplasmic male sterile 0A193-CMS inBrassica rapaL.

2016 ◽  
Vol 135 (6) ◽  
pp. 707-713 ◽  
Author(s):  
Qianxin Huang ◽  
Xiaoyang Xin ◽  
Yuan Guo ◽  
Shengwu Hu
Keyword(s):  
Cytological Observation ◽  
Male Sterile ◽  
Genetic Investigation ◽  
Cytoplasmic Male Sterile ◽  
New Type

scholarly journals Cytological observation of anther structure and genetic investigation of a thermo-sensitive genic male sterile line 373S in Brassica napus L

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yanyan Sun ◽  
Dongsuo Zhang ◽  
Zhenzhen Wang ◽  
Yuan Guo ◽  
Xiaomin Sun ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Spontaneous Mutation ◽  
Hybrid Breeding ◽  
Pcr Analysis ◽  
Cytological Observation ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Genetic Investigation ◽  
Temperature Changes

Abstract Background Photoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L. The present study characterized this TGMS line through cytological observation, photoperiod/ temperature treatments, and genetic investigation. Results Microscopic observation revealed that the condensed cytoplasm and irregular exine of microspores and the abnormal degradation of tapetum are related to pollen abortion. Different temperature and photoperiod treatments in field and growth cabinet conditions indicated that the fertility alteration of 373S was mainly caused by temperature changes. The effects of photoperiod and interaction between temperature and photoperiod were insignificant. The critical temperature leading to fertility alteration ranged from 10 °C (15 °C/5 °C) to 12 °C (17 °C/7 °C), and the temperature-responding stage was coincident with anther development from pollen mother cell formation to meiosis stages. Genetic analysis indicated that the TGMS trait in 373S was controlled by one pair of genes, with male sterility as the recessive. Multiplex PCR analysis revealed that the cytoplasm of 373S is pol type. Conclusions Our study suggested that the 373S line in B. napus has a novel thermo-sensitive gene Bnmst1 in Pol CMS cytoplasm background, and its fertility alteration is mainly caused by temperature changes. Our results will broaden the TGMS resources and lay the foundation for two-line hybrid breeding in B. napus.

scholarly journals Characterisation and expression of the mitochondrial genome of a new type of cytoplasmic male-sterile sunflower

1994 ◽  
Vol 26 (6) ◽  
pp. 1819-1831 ◽  
Author(s):  
Mariana Spassova ◽  
Fran�oise Moneger ◽  
Christopher J. Leaver ◽  
Peter Petrov ◽  
Atanas Atanassov ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
New Type

Cloning and Expression Analysis of cMDH Gene Related to Cytoplasmic Male Sterile Wheat with Aegilops kotschyi Cytoplasm

2009 ◽  
Vol 35 (9) ◽  
pp. 1620-1627 ◽  
Author(s):  
Long-Yu ZHANG ◽  
Hong-Xia LI ◽  
Gai-Sheng ZHANG ◽  
Jun-Sheng WANG ◽  
Yan-Fen HAN ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Cloning And Expression ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Cytological Observation of Partial Sterility of Nucleic Male Sterile Line ms86-1 in Sesame (Sesamum indicum)

2014 ◽  
Vol 49 (1) ◽  
pp. 87 ◽  
Author(s):  
Guo Wei ◽  
Miao Hongmei ◽  
Zhang Tide ◽  
Wei Libin ◽  
Li Chun ◽  
...  
Keyword(s):  
Sesamum Indicum ◽  
Cytological Observation ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Partial Sterility

scholarly journals Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinya Toriyama ◽  
Tomohiko Kazama ◽  
Tadashi Sato ◽  
Yoshimichi Fukuta ◽  
Masaaki Oka
Keyword(s):  
Nuclear Gene ◽  
Oryza Rufipogon ◽  
Rice Cultivars ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Abstract Background A cytoplasm of CW-type cytoplasmic male sterile (CMS) line is derived from Oryza rufipogon strain W1 and fertility is restored by a single nuclear gene, Rf17. We have previously reported that CW-CMS were effective for breeding CMS lines of Indica Group rice cultivars, IR 24 and IR 64. The applicability of this CW-CMS/Rf17 system to produce other elite Indica Group rice cultivars with CMS was explored. Findings Out of seven elite Indica Group rice cultivars, complete CMS lines were obtained for six cultivars: NSIC Rc 160, NSIC Rc 240, Ciherang, BRRI dhan 29, NERICA-L-19, and Pusa Basmati. The fertility of these six lines was restored when Rf17 was present. A CMS line was not obtained for the cultivar Samba Mahsuri. Conclusions The CW-CMS/Rf17 system will be useful to produce CMS lines and restorer lines of various elite Indica Group rice cultivars.

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