Interaction of common bacterial blight quantitative trait loci in a resistant inter-cross population of common bean

2013 ◽  
Vol 132 (6) ◽  
pp. 658-666 ◽  
Author(s):  
Kelli M. Durham ◽  
Weilong Xie ◽  
Kangfu Yu ◽  
K. Peter Pauls ◽  
Elizabeth Lee ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Bacterial Blight ◽  
Common Bacterial Blight ◽  
Trait Loci

scholarly journals Interaction of Common Bacterial Blight Bacteria with Disease Resistance Quantitative Trait Loci in Common Bean

2011 ◽  
Vol 101 (4) ◽  
pp. 425-435 ◽  
Author(s):  
Robert W. Duncan ◽  
Shree P. Singh ◽  
Robert L. Gilbertson
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Bacterial Blight ◽  
Brown Pigment ◽  
Common Bacterial Blight ◽  
Specific Primers ◽  
Geographical Regions ◽  
New Genotype ◽  
Trait Loci

Common bacterial blight (CBB) of common bean (Phaseolus vulgaris L.) is caused by Xanthomonas campestris pv. phaseoli and X. fuscans subsp. fuscans, and is the most important bacterial disease of this crop in many regions of the world. In 2005 and 2006, dark red kidney bean fields in a major bean-growing region in central Wisconsin were surveyed for CBB incidence and representative symptomatic leaves collected. Xanthomonad-like bacteria were isolated from these leaves and characterized based upon phenotypic (colony) characteristics, pathogenicity on common bean, polymerase chain reaction (PCR) with X. campestris pv. phaseoli- and X. fuscans subsp. fuscans-specific primers, and repetitive-element PCR (rep-PCR) and 16S-28S ribosomal RNA spacer region sequence analyses. Of 348 isolates that were characterized, 293 were identified as common blight bacteria (i.e., pathogenic on common bean and positive in PCR tests with the X. campestris pv. phaseoli- and X. fuscans subsp. fuscans-specific primers), whereas the other isolates were nonpathogenic xanthomonads. Most (98%) of the pathogenic xanthomonads were X. campestris pv. phaseoli, consistent with the association of this bacterium with CBB in large-seeded bean cultivars of the Andean gene pool. Two types of X. campestris pv. phaseoli were involved with CBB in this region: typical X. campestris pv. phaseoli (P) isolates with yellow mucoid colonies, no brown pigment production, and a typical X. campestris pv. phaseoli rep-PCR fingerprint (60% of strains); and a new phenotype and genotype (Px) with an X. campestris pv. phaseoli-type fingerprint and less mucoid colonies that produced brown pigment (40% of strains). In addition, a small number of X. fuscans subsp. fuscans strains, representing a new genotype (FH), were isolated from two fields in 2005. Representative P and Px X. campestris pv. phaseoli strains, an FH X. fuscans subsp. fuscans strain, plus five previously characterized X. campestris pv. phaseoli and X. fuscans subsp. fuscans genotypes were inoculated onto 28 common bean genotypes having various combinations of known CBB resistance quantitative trait loci (QTL) and associated sequence-characterized amplified region markers. Different levels of virulence were observed for X. campestris pv. phaseoli strains, whereas X. fuscans subsp. fuscans strains were similar in virulence. The typical X. campestris pv. phaseoli strain from Wisconsin was most virulent, whereas X. campestris pv. phaseoli genotypes from East Africa were the least virulent. Host genotypes having the SU91 marker-associated resistance and one or more other QTL (i.e., pyramided resistance), such as the VAX lines, were highly resistant to all genotypes of common blight bacteria tested. This information will help in the development of CBB resistance-breeding strategies for different common bean market classes in different geographical regions, as well as the identification of appropriate pathogen genotypes for screening for resistance.

scholarly journals Bacterial, Fungal, and Viral Disease Resistance Loci Mapped in a Recombinant Inbred Common Bean Population (`Dorado'/XAN 176)

2000 ◽  
Vol 125 (4) ◽  
pp. 476-481 ◽  
Author(s):  
Phillip N. Miklas ◽  
Richard Delorme ◽  
Valerie Stone ◽  
Mark J. Daly ◽  
J. Rennie Stavely ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Scar Markers ◽  
Common Bacterial Blight ◽  
Linkage Groups ◽  
Stem Blight

Understanding the genomic associations among disease resistance loci will facilitate breeding of multiple disease resistant cultivars. We constructed a genetic linkage map in common bean (Phaseolus vulgaris L.) containing six genes and nine quantitative trait loci (QTL) comprising resistance to one bacterial, three fungal, and two viral pathogens of bean. The mapping population consisted of 79 F5:7 recombinant inbred lines (RILs) derived from a `Dorado'/XAN 176 hybridization. There were 147 randomly amplified polymorphic DNA (RAPD) markers, two sequence characterized amplified region (SCAR) markers, one intersimple sequence repeat (ISSR) marker, two seedcoat color genes R and V, the Asp gene conditioning seed brilliance, and two rust [Uromyces appendiculatus var. appendiculatus (Pers.:Pers) Unger] resistance genes: one conditioning resistance to Races 53 and 54 and the other conditioning resistance to Race 108. These markers mapped across eleven linkage groups, one linked triad, and seven linked pairs for an overall map length of 930 cM (Kosambi). Genes conditioning resistance to anthracnose (Co-2) [Colletotrichum lindemuthianum (Sacc. and Magnus) Lams.-Scrib.], bean rust (Ur-5), and bean common mosaic virus (I and bc-3) (BCMV) did not segregate in this population, but were mapped by inference using linked RAPD and SCAR markers identified in other populations. Nine previously reported quantitative trait loci (QTL) conditioning resistance to a variety of pathogens including common bacterial blight [Xanthomonas campestris pv. phaseoli (Smith) Dye], ashy stem blight [Macrophomina phaseolina (Tassi) Goid.], and bean golden mosaic virus (BGMV), were located across four linkage groups. Linkage among QTL for resistance to ashy stem blight, BGMV, and common bacterial blight on linkage group B7 and ashy stem blight, BGMV, and rust resistance loci on B4 will complicate breeding for combined resistance to all four pathogens in this population.

Quantitative Trait Loci for Root Architecture Traits Correlated with Phosphorus Acquisition in Common Bean

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 413-423 ◽  
Author(s):  
Stephen E. Beebe ◽  
Marcela Rojas‐Pierce ◽  
Xiaolong Yan ◽  
Matthew W. Blair ◽  
Fabio Pedraza ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Root Architecture ◽  
Phosphorus Acquisition ◽  
Trait Loci

scholarly journals Identification of Pathotypes of Xanthomonas axonopodis pv. manihotis in Africa and Detection of Quantitative Trait Loci and Markers for Resistance to Bacterial Blight of Cassava

2004 ◽  
Vol 94 (10) ◽  
pp. 1084-1093 ◽  
Author(s):  
K. Wydra ◽  
V. Zinsou ◽  
V. Jorge ◽  
V. Verdier
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Bacterial Blight ◽  
Mapping Population ◽  
Female Parent ◽  
Phenotypic Variance ◽  
Xanthomonas Axonopodis ◽  
Progress Curve ◽  
Trait Loci ◽  
Moderately Resistant

Cassava suffers from bacterial blight attack in all growing regions. Control by resistance is unstable due to high genotype-environment interactions. Identifying genes for resistance to African strains of Xanthomonas axonopodis pv. manihotis can support breeding efforts. Five F1 cassava genotypes deriving from the male parent ‘CM2177-2’ and the female parent ‘TMS30572’ were used to produce 111 individuals by backcrossing to the female parent. In all, 16 genotypes among the mapping population were resistant to stem inoculation by four strains of X. axonopodis pv. manihotis from different locations in Africa, and 19 groups with differential reactions to the four strains were identified, suggesting that the strains represent different pathotypes. Four genotypes were resistant to leaf inoculation, and three were resistant to both stem and leaf inoculations. Genotypes with susceptible, moderately resistant, and resistant reactions after leaf and stem inoculation partly differed in their reactions on leaves and stems. Based on the genetic map of cassava, single-markeranalysis of disease severity after stem-puncture inoculation was performed. Eleven markers were identified, explaining between 16 and 33.3% of phenotypic variance of area under disease progress curve. Five markers on three and one linkage groups from the female- and male-derived framework of family CM8820, respectively, seem to be weakly associated with resistance to four strains of X. axonopodis pv. manihotis. Based on the segregation of alleles from the female of family CM8873, one marker was significantly associated with resistance to two X. axonopodis pv. manihotis strains, GSPB2506 and GSPB2511, whereas five markers were not linked to any linkage group. The quantitative trait loci (QTL) mapping results also suggest that the four African strains belong to four different pathotypes. The identified pathotypes should be useful for screening for resistance, and the QTL and markers will support breeding for resistance.

Mapping quantitative trait loci conferring partial physiological resistance to white mold in the common bean RIL population Xana × Cornell 49242

2010 ◽  
Vol 29 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Elena Pérez-Vega ◽  
Aida Pascual ◽  
Ana Campa ◽  
Ramón Giraldez ◽  
Phillip N. Miklas ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
White Mold ◽  
Ril Population ◽  
Trait Loci ◽  
Physiological Resistance ◽  
The Common

scholarly journals Two Independent Quantitative Trait Loci Are Responsible for Novel Resistance to Beet curly top virus in Common Bean Landrace G122

2010 ◽  
Vol 100 (10) ◽  
pp. 972-978 ◽  
Author(s):  
Richard C. Larsen ◽  
Chester J. Kurowski ◽  
Phillip N. Miklas
Keyword(s):  
Quantitative Trait Loci ◽  
Linkage Group ◽  
Common Bean ◽  
Quantitative Trait ◽  
Rapd Marker ◽  
The United States ◽  
Effective Control ◽  
Minor Effect ◽  
Beet Curly Top Virus ◽  
Trait Loci

Beet curly top virus, often referred to as Curly top virus (CTV), is an important virus disease of common bean in the semiarid regions of the United States, Canada, and Mexico and the only effective control is genetic resistance. Our objective was to determine if dry bean landrace G122, which lacks the Bct gene for resistance to CTV, contains novel resistance to the virus. Two populations, GT-A and GT-B, consisting of 98 F5:7 recombinant inbred lines (RILs) in total were derived from a cross between G122 and the susceptible variety Taylor Horticultural and evaluated for phenotypic response to natural CTV field infection. Genetic analyses revealed random amplified polymorphism DNA (RAPD) markers associated with a major-effect quantitative trait loci (QTL) from G122 which exhibited stable expression across 3 years in both populations. Phenotypic variation explained by the QTL in GT-A (37.6%) was greater than in GT-B (20.4%). RAPD marker Q14.973 was converted to a sequence-characterized amplified region (SCAR) and designated SQ14.973. The SCAR was used to locate the QTL on linkage group 6 of the Phaseolus core map. A survey of 74 common bean cultivars and breeding lines revealed SQ14.973 would be widely useful for marker-assisted selection of the QTL. An additional minor-effect QTL from G122 was detected on linkage group 7. G122 was determined to possess novel resistance to CTV conditioned by at least two genes, one with major the other minor effect.

Genetic variation underlying pod size and color traits of common bean depends on quantitative trait loci with epistatic effects

2014 ◽  
Vol 33 (4) ◽  
pp. 939-952 ◽  
Author(s):  
Fernando J. Yuste-Lisbona ◽  
Ana M. González ◽  
Carmen Capel ◽  
Manuel García-Alcázar ◽  
Juan Capel ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Epistatic Effects ◽  
Color Traits ◽  
Trait Loci

Putative Quantitative Trait Loci for Physical and Chemical Components of Common Bean

Crop Science ◽  
2003 ◽  
Vol 43 (3) ◽  
pp. 1029-1035 ◽  
Author(s):  
Salvador H. Guzmán‐Maldonado ◽  
Octavio Martínez ◽  
Jorge A. Acosta‐Gallegos ◽  
Fidel Guevara‐Lara ◽  
Octavio Paredes‐López
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
Chemical Components ◽  
Trait Loci ◽  
Physical And Chemical
Sign in / Sign up

Export Citation Format

Share Document