scholarly journals In vivo maternal haploid induction in tomato

2021 ◽  
Author(s):  
Yu Zhong ◽  
Baojian Chen ◽  
Dong Wang ◽  
Xijian Zhu ◽  
Mengran Li ◽  
...  
Keyword(s):  
Haploid Induction

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

In Vivo Haploid Induction in Maize: Comparison of Different Testing Regimes for Measuring Haploid Induction Rates

Crop Science ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 1127-1135 ◽  
Author(s):  
Albrecht E. Melchinger ◽  
Pedro Correa Brauner ◽  
Juliane Böhm ◽  
Wolfgang Schipprack
Keyword(s):  
Haploid Induction

scholarly journals Extension of thein vivohaploid induction system from maize to wheat

2019 ◽  
Author(s):  
Chenxu Liu ◽  
Yu Zhong ◽  
Xiaolong Qi ◽  
Ming Chen ◽  
Zongkai Liu ◽  
...  
Keyword(s):  
Pollen Viability ◽  
Haploid Induction ◽  
Male Sterile ◽  
Crop Breeding ◽  
Crop Species ◽  
Ploidy Levels ◽  
New Approach ◽  
Knock Out ◽  
Haploid Production

AbstractDoubled haploid breeding technology has been one of the most important techniques for accelerating crop breeding. In compare toin vivohaploid induction in maize, which is efficient and background independent, wheat haploid production by interspecific hybridization pollinated with maize is influenced by genetic background and requires rescue of young embryos. Here, we analyzed the homologues of maize haploid induction geneMTL/ZmPLA1/NLDin several crop species systematically, the homologues are highly conserved in sorghum, millet and wheat etc. Since wheat is a very important polyploidy crop, as a proof of concept, we demonstrated that thein vivohaploid induction method could be extended from diploid maize to hexaploid wheat by knocking out the wheat homologues (TaPLAs). Result showed that double knock-out mutation could trigger wheat haploid induction at ~ 2%-3%, accompanied by 30% - 60% seed setting rate. The performance of haploid wheat individual showed shorter plant, narrower leaves and male sterile. Our results also revealed that knockout ofTaPLA-A andTaPLA-D do not affect pollen viability. This study not only confirmed the function of the induction gene and explored a new approach for haploid production in wheat, but also provided an example that thein vivohaploid induction could be applied in more crop species with different ploidy levels. Furthermore, by combining with gene editing, it would be a fast and powerful platform for traits improvement in polyploidy crops breeding.

scholarly journals Doubled haploid technology for line development in maize: technical advances and prospects

2019 ◽  
Vol 132 (12) ◽  
pp. 3227-3243 ◽  
Author(s):  
Vijay Chaikam ◽  
Willem Molenaar ◽  
Albrecht E. Melchinger ◽  
Prasanna M. Boddupalli
Keyword(s):  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Production Costs ◽  
Great Promise ◽  
Success Rates ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Breeding Programs ◽  
Line Production

Key Message Increased efficiencies achieved in different steps of DH line production offer greater benefits to maize breeding programs. Abstract Doubled haploid (DH) technology has become an integral part of many commercial maize breeding programs as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide. The established method for maize DH production covered in this review involves in vivo induction of maternal haploids by a male haploid inducer genotype, identification of haploids from diploids at the seed or seedling stage, chromosome doubling of haploid (D0) seedlings and finally, selfing of fertile D0 plants. Development of haploid inducers with high haploid induction rates and adaptation to different target environments have facilitated increased adoption of DH technology in the tropics. New marker systems for haploid identification, such as the red root marker and high oil marker, are being increasingly integrated into new haploid inducers and have the potential to make DH technology accessible in germplasm such as some Flint, landrace, or tropical material, where the standard R1-nj marker is inhibited. Automation holds great promise to further reduce the cost and time in haploid identification. Increasing success rates in chromosome doubling protocols and/or reducing environmental and human toxicity of chromosome doubling protocols, including research on genetic improvement in spontaneous chromosome doubling, have the potential to greatly reduce the production costs per DH line.

A DMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis

Nature Plants ◽  
2020 ◽  
Vol 6 (5) ◽  
pp. 466-472 ◽  
Author(s):  
Yu Zhong ◽  
Baojian Chen ◽  
Mengran Li ◽  
Dong Wang ◽  
Yanyan Jiao ◽  
...  
Keyword(s):  
Haploid Induction ◽  
Induction System

In Vivo Haploid Induction in Maize: Identification of Haploid Seeds by Their Oil Content

Crop Science ◽  
2014 ◽  
Vol 54 (4) ◽  
pp. 1497-1504 ◽  
Author(s):  
Albrecht E. Melchinger ◽  
Wolfgang Schipprack ◽  
H. Friedrich Utz ◽  
Vilson Mirdita
Keyword(s):  
Oil Content ◽  
Haploid Induction

Doubled Haploids in Tropical Maize: I. Effects of Inducers and Source Germplasm on in vivo Haploid Induction Rates

Crop Science ◽  
2011 ◽  
Vol 51 (4) ◽  
pp. 1498-1506 ◽  
Author(s):  
Vanessa Prigge ◽  
Ciro Sánchez ◽  
Baldev S. Dhillon ◽  
Wolfgang Schipprack ◽  
José Luis Araus ◽  
...  
Keyword(s):  
Doubled Haploids ◽  
Tropical Maize ◽  
Haploid Induction

scholarly journals A genotype independent DMP-HI system in dicot crops

2021 ◽  
Author(s):  
Yu Zhong ◽  
Baojian Chen ◽  
Dong Wang ◽  
Xijian Zhu ◽  
Yuwen Wang ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Haploid Induction ◽  
Crop Breeding ◽  
Single Generation ◽  
The Way

Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, which significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is been widely and efficiently used in maize and was recently extended to several monocot crops. However, a similar generic and efficient HI system is still lacking in dicot crops. Here we show that genotype-independent in vivo HI can be triggered by mutation of DMP genes in tomato, rapeseed and tobacco with HI rates of ~1.9%, 2.4% and 1.2%, respectively. The DMP-HI system offers a robust DH technology to facilitate variety improvement in these crops. The success of this approach and the conservation of DMP genes paves the way for a generic and efficient genotype-independent HI system in other dicot crops.

scholarly journals In vivo haploid induction leads to increased frequency of twin-embryo and abnormal fertilization in maize

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Liwei Liu ◽  
Wei Li ◽  
Chenxu Liu ◽  
Baojian Chen ◽  
Xiaolong Tian ◽  
...  
Keyword(s):  
Haploid Induction ◽  
Abnormal Fertilization

scholarly journals Extension of the in vivo haploid induction system from diploid maize to hexaploid wheat

2019 ◽  
Vol 18 (2) ◽  
pp. 316-318 ◽  
Author(s):  
Chenxu Liu ◽  
Yu Zhong ◽  
Xiaolong Qi ◽  
Ming Chen ◽  
Zongkai Liu ◽  
...  
Keyword(s):  
Hexaploid Wheat ◽  
Haploid Induction ◽  
Induction System
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