Mediation of JA signalling in glandular trichomes by the woolly/SlMYC1 regulatory module improves pest resistance in tomato

Introgression of the sesquiterpene biosynthesis from Solanum habrochaites to cultivated tomato offers insights into trichome morphology and arthropod resistance

Planta ◽

10.1007/s00425-021-03651-y ◽

2021 ◽

Vol 254 (1) ◽

Author(s):

Rodrigo Therezan ◽

Ruy Kortbeek ◽

Eloisa Vendemiatti ◽

Saioa Legarrea ◽

Severino M. de Alencar ◽

...

Keyword(s):

Glandular Trichomes ◽

Pest Resistance ◽

Chromosome 8 ◽

Cavity Size ◽

Solanum Habrochaites ◽

Genetic Components ◽

Internal Storage ◽

In The Wild ◽

Low Levels ◽

Trichome Morphology

Abstract Main conclusion Cultivated tomatoes harboring the plastid-derived sesquiterpenes from S. habrochaites have altered type-VI trichome morphology and unveil additional genetic components necessary for piercing-sucking pest resistance. Abstract Arthropod resistance in the tomato wild relative Solanum habrochaites LA1777 is linked to specific sesquiterpene biosynthesis. The Sesquiterpene synthase 2 (SsT2) gene cluster on LA1777 chromosome 8 controls plastid-derived sesquiterpene synthesis. The main genes at SsT2 are Z-prenyltransferase (zFPS) and Santalene and Bergamotene Synthase (SBS), which produce α-santalene, β-bergamotene, and α-bergamotene in LA1777 round-shaped type-VI glandular trichomes. Cultivated tomatoes have mushroom-shaped type-VI trichomes with much smaller glands that contain low levels of monoterpenes and cytosolic-derived sesquiterpenes, not presenting the same pest resistance as in LA1777. We successfully transferred zFPS and SBS from LA1777 to cultivated tomato (cv. Micro-Tom, MT) by a backcrossing approach. The trichomes of the MT-Sst2 introgressed line produced high levels of the plastid-derived sesquiterpenes. The type-VI trichome internal storage-cavity size increased in MT-Sst2, probably as an effect of the increased amount of sesquiterpenes, although it was not enough to mimic the round-shaped LA1777 trichomes. The presence of high amounts of plastid-derived sesquiterpenes was also not sufficient to confer resistance to various tomato piercing-sucking pests, indicating that the effect of the sesquiterpenes found in the wild S. habrochaites can be insect specific. Our results provide for a better understanding of the morphology of S. habrochaites type-VI trichomes and paves the way to obtain insect-resistant tomatoes.

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Species Distribution of Biochemical and Morphological Characters Associated with Small Pest Resistance in Pelargonium ×hortorum

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.120.2.336 ◽

1995 ◽

Vol 120 (2) ◽

pp. 336-342 ◽

Cited By ~ 3

Author(s):

Richard Grazzini ◽

David Hesk ◽

Ellen Yerger ◽

Diana Cox-Foster ◽

June Medford ◽

...

Keyword(s):

Species Distribution ◽

Glandular Trichomes ◽

Pest Resistance ◽

Evolutionary Model ◽

Morphological Characters ◽

Anacardic Acids

Biochemical and morphological components of 16 Pelargonium species and the P. ×hortorum interspecific complex were examined. Inflorescences and leaves of each species were analyzed for anacardic acids and the presence of glandular trichomes. Three species of the section Ciconium, P. acetosum, P. frutetorum, and P. inquinans, produced anacardic acids in association with glandular trichomes. only P. inquinans and P. frutetorum contained ω5- anacardic acids. An evolutionary model for the origin of anacardic acids and ω5- desaturation is proposed.

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GLANDULAR TRICHOMES ON COTYLEDONARY PETIOLES OF LEGUMINOSAE TRIBE TRIFOLIEAE

Canadian Journal of Plant Science ◽

10.4141/cjps86-131 ◽

1986 ◽

Vol 66 (4) ◽

pp. 1019-1023 ◽

Cited By ~ 2

Author(s):

ERNEST SMALL ◽

BRENDA S. BROOKES

Keyword(s):

Insect Resistance ◽

Glandular Trichomes ◽

Pest Resistance ◽

Glandular Hairs ◽

Number Of Species ◽

Cotyledonary Petioles

Gland-tipped trichomes were found to occur on the cotyledonary petioles of a number of species of Medicago, Trigonella and Melilotus. Their location, seemingly protecting the plumule, and the known insect resistance provided by glandular hairs on stems and fruits of Medicago suggest that they are defensive, and may be useful in plant breeding.Key words: Medicago, alfalfa, Trigonella, Melilotus, glandular trichomes, pest resistance (immunity)

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An investigation into the ultrastructure and histochemistry of glandular trichomes of johnsongrass [Sorghum halepense (L.) Pers.] leaves

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124616 ◽

1992 ◽

Vol 50 (1) ◽

pp. 842-843 ◽

Cited By ~ 1

Author(s):

R. N. Paul ◽

C. G. McWhorter ◽

J.C. Ouzts

Keyword(s):

Glandular Trichomes ◽

Sorghum Halepense ◽

Secretory Apparatus

Secretory trichomes have been described on johnsongrass leaves previously, but there is little knowledge of their ultrastructure or the nature of their secretion. Since johnsongrass is a pernicious weed, there is much interest in the nature of compounds found on its foliar surface, and the possible effect of these extrusions on herbicide efficiency. This report describes the results of an investigation into the ultrastructure of the secretory apparatus, and into the nature of the secretion.

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Pest resistance feared as farmers flout rules

Nature ◽

10.1038/news030707-7 ◽

2003 ◽

Author(s):

Tom Clarke

Keyword(s):

Pest Resistance

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USE OF CELL AND MOLECULAR GENETIC MANIPULATION TO IMPROVE PASTURE PLANTS

Proceedings of the New Zealand Grassland Association ◽

10.33584/jnzg.1988.49.1828 ◽

1988 ◽

pp. 67-72

Author(s):

D.W.R. White

Keyword(s):

Cell Culture ◽

Perennial Ryegrass ◽

White Clover ◽

Nutritional Quality ◽

Rust Resistance ◽

Herbicide Tolerance ◽

Pest Resistance ◽

Crown Rust ◽

Wide Range ◽

Pasture Plants

Cell culture and genetic engineering techniques can be used to develop improved pasture plants. To utilise these methods we have developed procedures for regenerating plants from tissue cultures of perennial ryegrass and white clover. In both, the plant genotype influences regeneration capacity. There was significant genetic variation among regenerated perennial ryegrass plants in a wide range of characteristics. Most of the regenerants were resIstant to crown rust and this trait was highly heritable. This rust resistance is being used to breed a new ryegrass cultivar. A system for introducing cloned genes into white clover is described. This capability is bemg used to incorporate genes with the potential to improve nutritional quality and pest resistance. Other possibilities for engineering genetic improvements in white clover, genes conferring herbicide tolerance and resistance to white clover mosaic virus, are briefly outlined. Keywords: Lolium perenne, Trifolium repens, cell culture, somaclonal variation, crown rust resistance, transformation, cloned genes, nutritional quality, proteinase inhibitors, Bt toxins, pest resistance, WCMV viral cross-protection, herbicide tolerance, Agrobacterium, Bacillus thuringenisis.

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