scholarly journals The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome editing in allotetraploid cotton ( G. hirsutum ) and creation of nontransgenic, gossypol‐free cotton

2020 ◽  
Author(s):  
Bo Li ◽  
Sijia Liang ◽  
Muna Alariqi ◽  
Fuqiu Wang ◽  
Guanying Wang ◽  
...  
Keyword(s):  
Genome Editing ◽  
Temperature Sensitivity ◽  
Allotetraploid Cotton

scholarly journals Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

BMC Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Aimee A. Malzahn ◽  
Xu Tang ◽  
Keunsub Lee ◽  
Qiurong Ren ◽  
Simon Sretenovic ◽  
...  
Keyword(s):  
Genome Editing ◽  
Temperature Sensitivity

scholarly journals High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum ) using CRISPR/Cas9 system

2017 ◽  
Vol 16 (1) ◽  
pp. 137-150 ◽  
Author(s):  
Pengcheng Wang ◽  
Jun Zhang ◽  
Lin Sun ◽  
Yizan Ma ◽  
Jiao Xu ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genome Editing ◽  
High Efficient ◽  
Allotetraploid Cotton

scholarly journals Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

2022 ◽  
Vol 3 ◽  
Author(s):  
Raviraj Banakar ◽  
Mollie Schubert ◽  
Gavin Kurgan ◽  
Krishan Mohan Rai ◽  
Sarah F. Beaudoin ◽  
...  
Keyword(s):  
Genome Editing ◽  
Temperature Sensitivity ◽  
Plant Transformation ◽  
Development Stage ◽  
Sequence Specificity ◽  
Setaria Viridis ◽  
Delivery Methods ◽  
Thlaspi Arvense ◽  
Significant Temperature ◽  
Delivery Approach

Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native DNA fragments, independence of vector constructions, and less regulatory restrictions. Compared to the use in animal systems, RNP-mediated genome editing is still at the early development stage in plants. In this study, we established an efficient and simplified protoplast-based genome editing platform for CRISPR-Cas RNP delivery, and then evaluated the efficiency, specificity, and temperature sensitivity of six Cas9 and Cas12a proteins. Our results demonstrated that Cas9 and Cas12a RNP delivery resulted in genome editing frequencies (8.7–41.2%) at various temperature conditions, 22°C, 26°C, and 37°C, with no significant temperature sensitivity. LbCas12a often exhibited the highest activities, while AsCas12a demonstrated higher sequence specificity. The high activities of CRISPR-Cas RNPs at 22° and 26°C, the temperature preferred by plant transformation and tissue culture, led to high mutagenesis efficiencies (34.0–85.2%) in the protoplast-regenerated calli and plants with the heritable mutants recovered in the next generation. This RNP delivery approach was further extended to pennycress (Thlaspi arvense), soybean (Glycine max) and Setaria viridis with up to 70.2% mutagenesis frequency. Together, this study sheds light on the choice of RNP reagents to achieve efficient transgene-free genome editing in plants.

scholarly journals Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton ( Gossypium hirsutum )

2019 ◽  
Vol 17 (10) ◽  
pp. 1862-1864 ◽  
Author(s):  
Bo Li ◽  
Hangping Rui ◽  
Yajun Li ◽  
Qiongqiong Wang ◽  
Muna Alariqi ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Genome Editing ◽  
Allotetraploid Cotton

Genome editing: Wollen wir das wirklich?

2017 ◽  
Vol 6 (3) ◽  
pp. 162-162
Author(s):  
Liane Kaufmann ◽  
Michael von Aster
Keyword(s):  
Genome Editing

Genome editing (CRISPR-Cas9) to identify and characterise functional variants determining metformin response

2018 ◽  
Author(s):  
M Keller ◽  
J Dalla-Riva ◽  
A Kurbasic ◽  
M Al-Majdoub ◽  
P Spegel ◽  
...  
Keyword(s):  
Genome Editing ◽  
Functional Variants
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