scholarly journals A novel strategy for creating a new system of third‐generation hybrid rice technology using a cytoplasmic sterility gene and a genic male‐sterile gene

2020 ◽  
Author(s):  
Shufeng Song ◽  
Tiankang Wang ◽  
Yixing Li ◽  
Jun Hu ◽  
Ruifeng Kan ◽  
...  
Keyword(s):  
Hybrid Rice ◽  
Third Generation ◽  
Male Sterile ◽  
Sterility Gene ◽  
Cytoplasmic Sterility ◽  
Novel Strategy ◽  
Generation Hybrid ◽  
New System

scholarly journals Construction of a weight-based seed sorting system for the third-generation hybrid rice

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jianxin Wu ◽  
Shijun Qiu ◽  
Menglong Wang ◽  
Chunjue Xu ◽  
Xing Wang Deng ◽  
...  
Keyword(s):  
Hybrid Rice ◽  
Pollen Grains ◽  
Third Generation ◽  
Male Sterile ◽  
Specific Expression ◽  
The Third ◽  
Genes Encoding ◽  
Wild Type Male ◽  
Sorting System ◽  
Generation Hybrid

Abstract Background The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds. Results Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available. Conclusions The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology.

scholarly journals Construction of a Weight-based Seed Sorting System for the Third-generation Hybrid Rice

2021 ◽  
Author(s):  
Jianxin Wu ◽  
Shijun Qiu ◽  
Menglong Wang ◽  
Chunjue Xu ◽  
Xing Wang Deng ◽  
...  
Keyword(s):  
Hybrid Rice ◽  
Pollen Grains ◽  
Third Generation ◽  
Male Sterile ◽  
Specific Expression ◽  
The Third ◽  
Genes Encoding ◽  
Wild Type Male ◽  
Sorting System ◽  
Generation Hybrid

Abstract Background: The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds.Results: Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available.Conclusions: The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology.

scholarly journals Innovation and development of the third-generation hybrid rice technology

2021 ◽  
Author(s):  
Chancan Liao ◽  
Wei Yan ◽  
Zhufeng Chen ◽  
Gang Xie ◽  
Xing Wang Deng ◽  
...  
Keyword(s):  
Hybrid Rice ◽  
Third Generation ◽  
The Third ◽  
Generation Hybrid ◽  
Innovation And Development

scholarly journals Comparison of epicardial and endocardial lead systems for an implantable third-generation hybrid cardioverter-defibrillator

1991 ◽  
Vol 17 (2) ◽  
pp. A55 ◽  
Author(s):  
Huanlin An ◽  
Sanjeev Saksena ◽  
Nicholas G. Tullo ◽  
Ryszard B. Krol ◽  
Edward Bnrkhardt ◽  
...  
Keyword(s):  
Third Generation ◽  
Cardioverter Defibrillator ◽  
Endocardial Lead ◽  
Generation Hybrid

scholarly journals Genomic Prediction of Yield Traits in Single-Cross Hybrid Rice (Oryza sativa L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Marlee R. Labroo ◽  
Jauhar Ali ◽  
M. Umair Aslam ◽  
Erik Jon de Asis ◽  
Madonna A. dela Paz ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Genomic Prediction ◽  
Hybrid Rice ◽  
Phenotypic Selection ◽  
Production Costs ◽  
Breeding Cycle ◽  
Male Sterile ◽  
Training Set ◽  
Combining Abilities ◽  
Genomic Predictions

Hybrid rice varieties can outyield the best inbred varieties by 15 – 30% with appropriate management. However, hybrid rice requires more inputs and management than inbred rice to realize a yield advantage in high-yielding environments. The development of stress-tolerant hybrid rice with lowered input requirements could increase hybrid rice yield relative to production costs. We used genomic prediction to evaluate the combining abilities of 564 stress-tolerant lines used to develop Green Super Rice with 13 male sterile lines of the International Rice Research Institute for yield-related traits. We also evaluated the performance of their F1 hybrids. We identified male sterile lines with good combining ability as well as F1 hybrids with potential further use in product development. For yield per plant, accuracies of genomic predictions of hybrid genetic values ranged from 0.490 to 0.822 in cross-validation if neither parent or up to both parents were included in the training set, and both general and specific combining abilities were modeled. The accuracy of phenotypic selection for hybrid yield per plant was 0.682. The accuracy of genomic predictions of male GCA for yield per plant was 0.241, while the accuracy of phenotypic selection was 0.562. At the observed accuracies, genomic prediction of hybrid genetic value could allow improved identification of high-performing single crosses. In a reciprocal recurrent genomic selection program with an accelerated breeding cycle, observed male GCA genomic prediction accuracies would lead to similar rates of genetic gain as phenotypic selection. It is likely that prediction accuracies of male GCA could be improved further by targeted expansion of the training set. Additionally, we tested the correlation of parental genetic distance with mid-parent heterosis in the phenotyped hybrids. We found the average mid-parent heterosis for yield per plant to be consistent with existing literature values at 32.0%. In the overall population of study, parental genetic distance was significantly negatively correlated with mid-parent heterosis for yield per plant (r = −0.131) and potential yield (r = −0.092), but within female families the correlations were non-significant and near zero. As such, positive parental genetic distance was not reliably associated with positive mid-parent heterosis.

Transgene flow to hybrid rice and its male-sterile lines

2007 ◽  
Vol 16 (4) ◽  
pp. 491-501 ◽  
Author(s):  
Shirong Jia ◽  
Feng Wang ◽  
Lei Shi ◽  
Qianhua Yuan ◽  
Wuge Liu ◽  
...  
Keyword(s):  
Hybrid Rice ◽  
Male Sterile ◽  
Transgene Flow
Sign in / Sign up

Export Citation Format

Share Document