scholarly journals Loss of function of CRT1a (calreticulin) reduces plant susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape ( Brassica napus )

2020 ◽  
Vol 18 (11) ◽  
pp. 2328-2344 ◽  
Author(s):  
Michael Pröbsting ◽  
Dirk Schenke ◽  
Roxana Hossain ◽  
Claudia Häder ◽  
Tim Thurau ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Loss Of Function ◽  
Verticillium Longisporum ◽  
Plant Susceptibility

scholarly journals Overexpression of Brassica napus MPK4 Enhances Resistance to Sclerotinia sclerotiorum in Oilseed Rape

2009 ◽  
Vol 22 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Zheng Wang ◽  
Han Mao ◽  
Caihua Dong ◽  
Ruiqin Ji ◽  
Li Cai ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Transgenic Plants ◽  
Oilseed Rape ◽  
Sclerotinia Sclerotiorum ◽  
Mitogen Activated Protein Kinase ◽  
Economic Losses ◽  
Loss Of Function ◽  
Mitogen Activated Protein ◽  
Transgenic Oilseed Rape ◽  
Plant Pathogen Interactions

Sclerotinia sclerotiorum causes a highly destructive disease in oilseed rape (Brassica napus) resulting in significant economic losses. Studies on the Arabidopsis thaliana MPK4 loss-of-function mutant have implicated that AtMPK4 is involved in plant defense regulation, and its effect on disease resistance varies in different plant–pathogen interactions. In this study, we isolated a B. napus mitogen-activated protein kinase, BnMPK4, and found that BnMPK4 along with PDF1.2 are inducible in resistant line Zhongshuang9 but both are consistently suppressed in susceptible line 84039 after inoculation with S. sclerotiorum. Transgenic oilseed rape overexpressing BnMPK4 markedly enhances resistance to S. sclerotiorum and Botrytis cinerea. Further experiments showed that transgenic plants inhibited growth of S. sclerotiorum and constitutively activated PDF1.2 but decreased H2O2 production and constitutively suppressed PR-1 expression. Treatment of roots of the transgenic plants with H2O2 solution resulted in enhanced susceptibility to the two pathogens. Our results support the idea that MPK4 positively regulates jasmonic acid-mediated defense response, which might play an important role in resistance to S. sclerotiorum in oilseed rape.

scholarly journals Broadening the Genetic Basis of Verticillium longisporum Resistance in Brassica napus by Interspecific Hybridization

2007 ◽  
Vol 97 (11) ◽  
pp. 1391-1396 ◽  
Author(s):  
W. Rygulla ◽  
R. J. Snowdon ◽  
C. Eynck ◽  
B. Koopmann ◽  
A. von Tiedemann ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Interspecific Hybridization ◽  
Oilseed Rape ◽  
Quantitative Resistance ◽  
Embryo Rescue ◽  
Diploid Species ◽  
Gene Bank ◽  
Oilseed Crop ◽  
Verticillium Longisporum ◽  
Interspecific Gene Transfer

Verticillium wilt caused by the vascular fungal pathogen Verticillium longisporum is one of the most important pathogens of oilseed rape (Brassica napus sp. oleifera) in northern Europe. Because production of this major oilseed crop is expanding rapidly and no approved fungicides are available for V. longisporum, long-term control of the disease can only be achieved with cultivars carrying effective quantitative resistance. However, very little resistance to V. longisporum is available within the gene pool of oilseed rape, meaning that interspecific gene transfer from related species is the only possibility for broadening levels of resistance in current varieties. The amphidiploid species B. napus can be resynthesized by crossing the two progenitor species Brassica oleracea and Brassica rapa, hence resistant accessions of these two diploid species can be used as resistance donors. In this study a total of 43 potential B. rapa and B. oleracea resistance donors were tested with regard to their reaction to a mixture of two aggressive V. longisporum isolates, and resistances from diverse lines were combined by embryo rescue-assisted interspecific hybridization in resynthesized rapeseed lines. Progenies from crosses of the two B. rapa gene bank accessions 13444 and 56515 to the B. oleracea gene bank accessions BRA1008, CGN14044, 8207, BRA1398, and 7518 showed a broad spectrum of resistance in pathogenicity tests. Of 45 tested resynthesized lines, 41 lines exhibited a significantly higher level of resistance than the moderately V. longisporum-tolerant oilseed rape cultivar Express. These lines represent a promising basis for the combination of different resistance resources in new varieties.

The genetics of stamenoid petal production in oilseed rape (Brassica napus) and equivalent variation in Arabidopsis thaliana

1997 ◽  
Vol 94 (6-7) ◽  
pp. 731-736 ◽  
Author(s):  
M. J. Fray ◽  
P. Puangsomlee ◽  
J. Goodrich ◽  
G. Coupland ◽  
E. J. Evans ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Equivalent Variation

scholarly journals Identification of Quantitative Trait Loci for Resistance Against Verticillium longisporum in Oilseed Rape (Brassica napus)

2008 ◽  
Vol 98 (2) ◽  
pp. 215-221 ◽  
Author(s):  
W. Rygulla ◽  
R. J. Snowdon ◽  
W. Friedt ◽  
I. Happstadius ◽  
W. Y. Cheung ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Quantitative Trait Loci ◽  
Oilseed Rape ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Fragment Length Polymorphism ◽  
Linkage Groups ◽  
Verticillium Longisporum ◽  
Dh Lines

Verticillium longisporum is one of the major pathogens of oilseed rape (Brassica napus; genome AACC, 2n = 38) in Europe. Current European cultivars possess only a low level of resistance against V. longisporum, meaning that heavy infection can cause major yield losses. The aim of this study was to identify quantitative trait loci (QTL) for resistance against V. longisporum as a starting point for marker-assisted breeding of resistant cultivars. Resistance QTL were localized in a segregating oilseed rape population of 163 doubled haploid (DH) lines derived by microspore culture from the F1 of a cross between two B. napus breeding lines, one of which exhibited V. longisporum resistance derived by pedigree selection from a resynthesized B. napus genotype. A genetic map was constructed comprising 165 restriction fragment length polymorphism, 94 amplified fragment length polymorphism and 45 simple sequence repeats (SSR) markers covering a total of 1,739 cM on 19 linkage groups. Seedlings of the DH lines and parents were inoculated with V. longisporum isolates in four greenhouse experiments performed in Sweden during autumn 1999. In three of the experiments the DH lines were inoculated with a mixture of five isolates, while in the fourth experiment only one of the isolates was used. The intention was to simulate four different environments with variable disease pressure, while still maintaining uniform conditions in each environment to enable reliable disease scoring. The disease index (DI) was calculated by scoring symptoms on a total of 21 inoculated plants per line in comparison to 21 noninoculated plants per line. Using the composite interval mapping procedure a total of four different chromosome regions could be identified that showed significant QTL for resistance in more than one environment. Two major QTL regions were identified on the C-genome linkage groups N14 and N15, respectively; each of these QTL consistently exhibited significant effects on resistance in multiple environments. The presence of flanking markers for the respective QTL was associated with a significant reduction in DI in the inoculated DH lines.

scholarly journals Suppression of abscisic acid biosynthesis at the early infection stage of Verticillium longisporum in oilseed rape ( Brassica napus )

2019 ◽  
Vol 20 (12) ◽  
pp. 1645-1661 ◽  
Author(s):  
Falk H. Behrens ◽  
Dirk Schenke ◽  
Roxana Hossain ◽  
Wanzhi Ye ◽  
Markus Schemmel ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Early Infection ◽  
Acid Biosynthesis ◽  
Abscisic Acid Biosynthesis ◽  
Verticillium Longisporum ◽  
Infection Stage

scholarly journals Defence reactions in the apoplastic proteome of oilseed rape (Brassica napus var. napus) attenuate Verticillium longisporum growth but not disease symptoms

2008 ◽  
Vol 8 (1) ◽  
pp. 129 ◽  
Author(s):  
Saskia Floerl ◽  
Christine Druebert ◽  
Andrzej Majcherczyk ◽  
Petr Karlovsky ◽  
Ursula Kues ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Disease Symptoms ◽  
Verticillium Longisporum ◽  
Defence Reactions
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