scholarly journals Somatic embryogenesis critical initiation stage‐specific m CHH hypomethylation reveals epigenetic basis underlying embryogenic redifferentiation in cotton

2020 ◽  
Vol 18 (8) ◽  
pp. 1648-1650 ◽  
Author(s):  
Huihui Guo ◽  
Yijie Fan ◽  
Haixia Guo ◽  
Jianfei Wu ◽  
Xiaoman Yu ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Initiation Stage ◽  
Critical Initiation

scholarly journals Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation

2019 ◽  
Vol 20 (7) ◽  
pp. 1691 ◽  
Author(s):  
◽  
Fanchang Zeng ◽  
◽  
◽  
◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Quantitative Proteomics ◽  
Large Scale ◽  
Biological Activities ◽  
Embryogenic Calli ◽  
Systematic Analysis ◽  
Dynamic Expression ◽  
Process Of Se ◽  
Differentially Abundant Proteins ◽  
Critical Initiation

The somatic embryogenesis (SE) process of plants, as one of the typical responses to abiotic stresses with hormone, occurs through the dynamic expression of different proteins that constitute a complex regulatory network in biological activities and promotes plant totipotency. Plant SE includes two critical stages: primary embryogenic calli redifferentiation and somatic embryos development initiation, which leads to totipotency. The isobaric labels tandem mass tags (TMT) large-scale and quantitative proteomics technique was used to identify the dynamic protein expression changes in nonembryogenic calli (NEC), primary embryogenic calli (PEC) and globular embryos (GEs) of cotton. A total of 9369 proteins (6730 quantified) were identified; 805, 295 and 1242 differentially accumulated proteins (DAPs) were identified in PEC versus NEC, GEs versus PEC and GEs versus NEC, respectively. Eight hundred and five differentially abundant proteins were identified, 309 of which were upregulated and 496 down regulated in PEC compared with NEC. Of the 295 DAPs identified between GEs and PEC, 174 and 121 proteins were up- and down regulated, respectively. Of 1242 differentially abundant proteins, 584 and 658 proteins were up- and down regulated, respectively, in GEs versus NEC. We have also complemented the authenticity and accuracy of the proteomic analysis. Systematic analysis indicated that peroxidase, photosynthesis, environment stresses response processes, nitrogen metabolism, phytohormone response/signal transduction, transcription/posttranscription and modification were involved in somatic embryogenesis. The results generated in this study demonstrate a proteomic molecular basis and provide a valuable foundation for further investigation of the roles of DAPs in the process of SE transdifferentiation during cotton totipotency.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

Influence of long-term fertilization on soil microbial biomass and dehydrogenase activity in relation to crop productivity in an acid Inceptisols

2019 ◽  
Vol 56 (3) ◽  
pp. 305-311
Author(s):  
Debasis Purohit ◽  
Mitali Mandal ◽  
Avisek Dash ◽  
Kumbha Karna Rout ◽  
Narayan Panda ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Dehydrogenase Activity ◽  
Nutrient Content ◽  
Crop Productivity ◽  
Maturity Stage ◽  
Microbial Biomass Nitrogen ◽  
Biological Potential ◽  
Initiation Stage ◽  
The Impact

An effective approach for improving nutrient use efficiency and crop productivity simultaneously through exploitation of biological potential for efficient acquisition and utilization of nutrients by crops is very much needed in this current era. Thus, an attempt is made here to investigate the impact of long term fertilization in the soil ecology in rice-rice cropping system in post kharif - 2015 in flooded tropical rice (Oryza sativa L.) in an acidic sandy soil. The experiment was laid out in a randomized block design with quadruplicated treatments. Soil samples at different growth stages of rice were collected from long term fertilizer experiment.The studied long-term manured treatments included 100 % N, 100% NP, 100 % NPK, 150 % NPK and 100 % NPK+FYM (5 t ha-1) and an unmanured control. Soil fertility status like SOC content and other available nutrient content has decreased continuously towards the crop growth period. Comparing the results of different treatments, it was found that the application of 100% NPK + FYM exhibited highest nutrient content in soils. With regards to microbial properties it was also observed that the amount of microbial biomass carbon (MBC) and microbial biomass nitrogen ( MBN) showed highest accumulation in 100 % NPK + FYM at maximum tillering stage of the rice. The results further reveal that dehydrogenase activity was maximum at panicle initiation stage and thereafter it decreases. Soil organic carbon content, MBC, MBN and dehydrogenase activity were significantly correlated with each other. Significant correlations were observed between rice yield and MBC at maturity stage( R2 = 0.94**) and panicle initiation stage( R2 = 0.92**) and available nitrogen content at maturity stage( R2 = 0.91**).

Evolving appropriate zinc fertilization strategy for rice-rice (Oryza sativa) cropping system in Cauvery Delta Zone

2019 ◽  
Vol 56 (3) ◽  
pp. 294-304
Author(s):  
C Sharmila Rahale
Keyword(s):  
Oryza Sativa ◽  
Tamil Nadu ◽  
Field Experiments ◽  
Foliar Spray ◽  
Cropping System ◽  
Green Leaf ◽  
Farm Yard Manure ◽  
Zinc Fertilization ◽  
Initiation Stage ◽  
Cauvery Delta

Six field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai to evolve suitable zinc fertilization method for rice - rice (Oryza sativa L.) cropping system in Cauvery delta zone. The treatment includes: T1 : Control, T2 : 100 g zinc sulphate (ZnSO4)/cent in nursery alone., T3 : root dipping alone in 2 % zinc oxide (ZnO) solution, T4 : 25 kg ZnSO4 ha-1, T5 : 37.5 kg ZnSO4 ha-1, T6 : 25 kg ZnSO4 ha-1+ Farm Yard Manure (FYM) 12.5 t ha-1, T7 : 25 kg ZnSO4 ha-1 + Green Leaf Manure (GLM) 6.5 t ha-1, T8 : Tamil Nadu Agricultural University Micro Nutrient (TNAU MN) mixture 25 kg ha-1 as Enriched Farm Yard Manure (EFYM), T9 : TNAU MN mixture 37.5 kg ha-1 as EFYM, T10 : Foliar spray of 0.5% ZnSO4 + 1 % urea at tillering and panicle initiation stage, T11 : 100g ZnSO4 /cent in nursery alone + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2+ T10), T12 : root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T3 + T10), T13: 100 g ZnSO4 /cent in nursery alone + root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2 + T3 + T10). The treatments T5, T6, T7 and T9 were skipped in rabi season to know the residual effect of these treatments in the subsequent season. Among the treatment combinations, application of 25 kg ZnSO4 ha-1 + FYM 12.5 t ha-1 recorded higher grain yield in both kharif (6232 kg ha-1) and rabi (6236 kg ha-1) seasons. The same treatment combination recorded higher Zn content and Zn uptake as well. Regarding soil nutrient content, the same treatment recorded higher N, P and K content. This treatment was followed by application of 25 kg ZnSO4 ha-1 + green leaf manure 6.5 t ha-1. The experimental findings suggested that combination of organic and inorganic sources not only increased the yield but also improves soil health in Cauvery delta zone.

Somatic Embryogenesis and Plant Development in Eight Cultivars of Pecan

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 573-576 ◽  
Author(s):  
I.E. Yates ◽  
C.C. Reilly
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Development ◽  
Fruit Development ◽  
Somatic Embryos ◽  
Carya Illinoensis ◽  
Appreciable Variation

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.

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