scholarly journals Efficient genetic transformation and CRISPR /Cas9‐mediated genome editing in Lemna aequinoctialis

2019 ◽  
Vol 17 (11) ◽  
pp. 2143-2152 ◽  
Author(s):  
Yu Liu ◽  
Yu Wang ◽  
Shuqing Xu ◽  
Xianfeng Tang ◽  
Jinshan Zhao ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Genome Editing ◽  
Efficient Genetic Transformation

scholarly journals Efficient Genetic Transformation of Rice for CRISPR/Cas9 Mediated Genome-Editing and Stable Overexpression Studies: A Case Study on Rice Lipase 1 and Galactinol Synthase Encoding Genes

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 179
Author(s):  
Tanika Thakur ◽  
Kshitija Sinha ◽  
Tushpinder Kaur ◽  
Ritu Kapoor ◽  
Gulshan Kumar ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Crop Improvement ◽  
Rice Cultivar ◽  
Climatic Conditions ◽  
Rice Transformation ◽  
Transformation Protocol ◽  
Galactinol Synthase ◽  
Biotic Stresses ◽  
Putative Transformants ◽  
Efficient Genetic Transformation

Rice is a staple food crop for almost half of the world’s population, especially in the developing countries of Asia and Africa. It is widely grown in different climatic conditions, depending on the quality of the water, soil, and genetic makeup of the rice cultivar. Many (a)biotic stresses severely curtail rice growth and development, with an eventual reduction in crop yield. However, for molecular functional analysis, the availability of an efficient genetic transformation protocol is essential. To ensure food security and safety for the continuously increasing global population, the development of climate-resilient crops is crucial. Here, in this study, the rice transformation protocol has been effectively optimized for the efficient and rapid generation of rice transgenic plants. We also highlighted the critical steps and precautionary measures to be taken while performing the rice transformation. We further assess the efficacy of this protocol by transforming rice with two different transformation constructs for generating galactinol synthase (GolS) overexpression lines and CRISPR/Cas9-mediated edited lines of lipase (Lip) encoding the OsLip1 gene. The putative transformants were subjected to molecular analysis to confirm gene integration/editing, respectively. Collectively, the easy, efficient, and rapid rice transformation protocol used in this present study can be applied as a potential tool for gene(s) function studies in rice and eventually to the rice crop improvement.

scholarly journals Establishment of an efficient genetic transformation method in Dunaliella tertiolecta mediated by Agrobacterium tumefaciens

2018 ◽  
Vol 150 ◽  
pp. 9-17 ◽  
Author(s):  
Claudia D. Norzagaray-Valenzuela ◽  
Lourdes J. Germán-Báez ◽  
Marco A. Valdez-Flores ◽  
Sergio Hernández-Verdugo ◽  
Luke M. Shelton ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Transformation Method ◽  
Dunaliella Tertiolecta ◽  
Efficient Genetic Transformation

scholarly journals In Planta Genetic Transformation to Produce CRISPRed High-Oleic Peanut

2021 ◽  
Author(s):  
Han Hong Wei ◽  
Shu Tao Yu ◽  
Zhi Wei Wang ◽  
Zhen Yang ◽  
Guo Sheng Song ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Genetic Transformation ◽  
Genome Editing ◽  
Loss Of Function ◽  
In Planta ◽  
Easy Method ◽  
High Oleic ◽  
Keeping Quality ◽  
Varietal Improvement ◽  
Multiple Health

Abstract In contrast to its normal-oleic counterpart, high-oleic peanut has better keeping quality and multiple health benefits. Breeding high-oleic peanut through conventional means is a tedious process generally requiring several years. Genome editing may shorten the duration. In this study, node injection method was used to transform normal-oleic Huayu 23, a popular peanut cultivar having loss-of-function FAD2A, with CRISPR/Cas9 construct targeting FAD2B, and two peanut mutants with over 80% oleic acid and 442A insertion in FAD2B were obtained. As a genotype-independent, simple and easy method for peanut genetic transformation, node injection has great potential in factional analysis of genes and in peanut varietal improvement.

scholarly journals An efficient genetic transformation method for glycerol producer Candida glycerinogenes

2008 ◽  
Vol 163 (5) ◽  
pp. 531-537 ◽  
Author(s):  
Xianzhong Chen ◽  
Huiying Fang ◽  
Zhiming Rao ◽  
Wei Shen ◽  
Bin Zhuge ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Transformation Method ◽  
Candida Glycerinogenes ◽  
Efficient Genetic Transformation

scholarly journals Efficient genetic transformation of Momordica charantia L. by microprojectile bombardment

3 Biotech ◽  
2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Muralikrishna Narra ◽  
Raghu Ellendula ◽  
Srinivas Kota ◽  
Bharathkumar Kalva ◽  
Yashodhara Velivela ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Microprojectile Bombardment ◽  
Momordica Charantia ◽  
Efficient Genetic Transformation

The efficient genetic transformation of Cordyceps militaris by using mononuclear protoplasts

2019 ◽  
Vol 243 ◽  
pp. 307-313 ◽  
Author(s):  
Hai-Wei Lou ◽  
Zhi-Wei Ye ◽  
Ying-Hao Yu ◽  
Jun-Fang Lin ◽  
Li-Qiong Guo ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Cordyceps Militaris ◽  
Efficient Genetic Transformation
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