scholarly journals Plant cell packs: a scalable platform for recombinant protein production and metabolic engineering

2019 ◽  
Vol 17 (8) ◽  
pp. 1560-1566 ◽  
Author(s):  
Thomas Rademacher ◽  
Markus Sack ◽  
Daniel Blessing ◽  
Rainer Fischer ◽  
Tanja Holland ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Recombinant Protein ◽  
Plant Cell ◽  
Recombinant Protein Production ◽  
Protein Production

Increasing recombinant protein production in Escherichia coli K12 through metabolic engineering

2013 ◽  
Vol 30 (2) ◽  
pp. 255-261 ◽  
Author(s):  
Hendrik Waegeman ◽  
Stijn De Lausnay ◽  
Joeri Beauprez ◽  
Jo Maertens ◽  
Marjan De Mey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Escherichia Coli K12

scholarly journals Optogenetic lac operon to control chemical and protein production in Escherichia coli with light

2019 ◽  
Author(s):  
Makoto A. Lalwani ◽  
Samantha S. Ip ◽  
Cesar Carrasco-Lopez ◽  
Evan M. Zhao ◽  
Hinako Kawabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Engineering ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Lac Operon ◽  
Iptg Induction ◽  
Spatial Control ◽  
Chemical Inducers ◽  
Cell Functions

AbstractControl of the lac operon with IPTG has been used for decades to regulate gene expression in E. coli for countless applications, including metabolic engineering and recombinant protein production. However, optogenetics offers unique capabilities such as easy tunability, reversibility, dynamic induction strength, and spatial control that are difficult to obtain with chemical inducers. We developed an optogenetic lac operon in a series of circuits we call OptoLAC. With these circuits, we control gene expression from various IPTG-inducible promoters using only blue light. Applying them to metabolic engineering improves mevalonate and isobutanol production by 24% and 27% respectively, compared to IPTG induction, in light-controlled fermentations scalable to at least 2L bioreactors. Furthermore, OptoLAC circuits enable light control of recombinant protein production, reaching yields comparable to IPTG induction, but with enhanced tunability of expression and spatial control. OptoLAC circuits are potentially useful to confer light controls over other cell functions originally engineered to be IPTG-inducible.

Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures

2020 ◽  
Vol 94 ◽  
pp. 103452 ◽  
Author(s):  
Bárbara A. Rebelo ◽  
Rita B. Santos ◽  
Osvaldo S. Ascenso ◽  
Ana Cláudia Nogueira ◽  
Diana Lousa ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Plant Cell ◽  
Cell Cultures ◽  
Small Molecule ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Biological Effects ◽  
Plant Cell Cultures ◽  
Small Molecule Enhancers
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