scholarly journals Single transcript unitCRISPR2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

2019 ◽  
Vol 17 (7) ◽  
pp. 1431-1445 ◽  
Author(s):  
Xu Tang ◽  
Qiurong Ren ◽  
Lijia Yang ◽  
Yu Bao ◽  
Zhaohui Zhong ◽  
...  
Keyword(s):  
Genome Editing ◽  
Plant Genome ◽  
Single Transcript

scholarly journals Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Zhaohui Zhong ◽  
Shishi Liu ◽  
Xiaopei Liu ◽  
Binglin Liu ◽  
Xu Tang ◽  
...  
Keyword(s):  
Genome Editing ◽  
Plant Genome ◽  
Single Transcript

An Era of CRISPR/ Cas9 Mediated Plant Genome Editing

2018 ◽  
pp. 47-54 ◽  
Author(s):  
Haris Khurshid ◽  
Sohail Ahmad Jan ◽  
Zabta Khan Shinwari ◽  
Muhammad Jamal ◽  
Sabir Hussain Shah
Keyword(s):  
Genome Editing ◽  
Plant Genome

scholarly journals Exosome/Liposome-like Nanoparticles: New Carriers for CRISPR Genome Editing in Plants

2021 ◽  
Vol 22 (14) ◽  
pp. 7456
Author(s):  
Mousa A. Alghuthaymi ◽  
Aftab Ahmad ◽  
Zulqurnain Khan ◽  
Sultan Habibullah Khan ◽  
Farah K. Ahmed ◽  
...  
Keyword(s):  
Genome Editing ◽  
Viral Vectors ◽  
Polymeric Materials ◽  
Inorganic Nanoparticles ◽  
Plant Genome ◽  
Delivery Methods ◽  
Detailed Consideration ◽  
Crispr System ◽  
Efficient Delivery ◽  
Low Cytotoxicity

Rapid developments in the field of plant genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems necessitate more detailed consideration of the delivery of the CRISPR system into plants. Successful and safe editing of plant genomes is partly based on efficient delivery of the CRISPR system. Along with the use of plasmids and viral vectors as cargo material for genome editing, non-viral vectors have also been considered for delivery purposes. These non-viral vectors can be made of a variety of materials, including inorganic nanoparticles, carbon nanotubes, liposomes, and protein- and peptide-based nanoparticles, as well as nanoscale polymeric materials. They have a decreased immune response, an advantage over viral vectors, and offer additional flexibility in their design, allowing them to be functionalized and targeted to specific sites in a biological system with low cytotoxicity. This review is dedicated to describing the delivery methods of CRISPR system into plants with emphasis on the use of non-viral vectors.

scholarly journals Knowing when to talk? Plant genome editing as a site for pre-engagement institutional reflexivity

2021 ◽  
pp. 096366252199979
Author(s):  
Robert D.J. Smith ◽  
Sarah Hartley ◽  
Patrick Middleton ◽  
Tracey Jewitt
Keyword(s):  
Genome Editing ◽  
Theory And Practice ◽  
Plant Genome ◽  
Limited Attention ◽  
Full Potential ◽  
Policy Makers ◽  
Institutional Dynamics ◽  
Social Studies Of Science ◽  
High Profile ◽  
A Site

Citizen and stakeholder engagement is frequently portrayed as vital for socially accountable science policy but there is a growing understanding of how institutional dynamics shape engagement exercises in ways that prevent them from realising their full potential. Limited attention has been devoted to developing the means to expose institutional features, allow policy-makers to reflect on how they will shape engagement and respond appropriately. Here, therefore, we develop and test a methodological framework to facilitate pre-engagement institutional reflexivity with one of the United Kingdom’s eminent science organisations as it grappled with a new, high-profile and politicised technology, genome editing. We show how this approach allowed policy-makers to reflect on their institutional position and enrich decision-making at a time when they faced pressure to legitimate decisions with engagement. Further descriptions of such pre-engagement institutional reflexivity are needed to better bridge theory and practice in the social studies of science.

Virus-induced plant genome editing

2021 ◽  
Vol 60 ◽  
pp. 101992
Author(s):  
Youngbin Oh ◽  
Hyeonjin Kim ◽  
Sang-Gyu Kim
Keyword(s):  
Genome Editing ◽  
Plant Genome

CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects

2016 ◽  
Vol 35 (7) ◽  
pp. 1417-1427 ◽  
Author(s):  
Joseph W. Paul ◽  
Yiping Qi
Keyword(s):  
Genome Editing ◽  
Plant Genome

New cytosine base editor for plant genome editing

2018 ◽  
Vol 61 (12) ◽  
pp. 1602-1603 ◽  
Author(s):  
Zhenxiang Li ◽  
Xiangyu Xiong ◽  
Jian-Feng Li
Keyword(s):  
Genome Editing ◽  
Plant Genome

scholarly journals Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

Plant Methods ◽  
2013 ◽  
Vol 9 (1) ◽  
pp. 39 ◽  
Author(s):  
Khaoula Belhaj ◽  
Angela Chaparro-Garcia ◽  
Sophien Kamoun ◽  
Vladimir Nekrasov
Keyword(s):  
Genome Editing ◽  
Crop Plants ◽  
Targeted Mutagenesis ◽  
Plant Genome
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