scholarly journals Novel technologies in doubled haploid line development

2017 ◽  
Vol 15 (11) ◽  
pp. 1361-1370 ◽  
Author(s):  
Jiaojiao Ren ◽  
Penghao Wu ◽  
Benjamin Trampe ◽  
Xiaolong Tian ◽  
Thomas Lübberstedt ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Line ◽  
Novel Technologies

scholarly journals Registration of Sugarbeet Doubled Haploid Line KDH13 with Resistance to Beet Curly Top

2016 ◽  
Vol 10 (1) ◽  
pp. 93-96 ◽  
Author(s):  
Imad Eujayl ◽  
Carl Strausbaugh ◽  
Chunsheng Lu
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Line

Agrobacterium -mediated transformation of a doubled haploid line of cabbage

2002 ◽  
Vol 21 (3) ◽  
pp. 257-262 ◽  
Author(s):  
Tsukazaki H. ◽  
Kuginuki Y. ◽  
Aida R. ◽  
Suzuki T.
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Line ◽  
Agrobacterium Mediated Transformation

scholarly journals Optimization of Cucumber Doubled Haploid Line Production Using In Vitro Rescue of In Vivo Induced Parthenogenic Embryos

2005 ◽  
Vol 130 (4) ◽  
pp. 555-560 ◽  
Author(s):  
Elisabet Claveria ◽  
Jordi Garcia-Mas ◽  
Ramon Dolcet-Sanjuan
Keyword(s):  
Flow Cytometry ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Doubled Haploid Line ◽  
High Rate ◽  
Limiting Factors ◽  
Line Production ◽  
Haploid Plants

Homozygous doubled haploid lines (DHLs) from new cucumber (Cucumis sativus L.) accessions could be useful to accelerate breeding for resistant varieties. DHLs have been generated by in vitro rescue of in vivo induced parthenogenic embryos. The protocol developed involves the following: 1) induction of parthenogenic embryos by pollinating with pollen irradiated with a Co60 γ-ray source at 500 Gy; 2) in vitro rescue of putative parthenogenic embryos identified by their morphology and localized using a dissecting scope or X-ray radiography; 3) discrimination of undesirable zygotic individuals from the homozygous plants using cucumber and melon SSR markers; 4) determination of ploidy level from homozygous plants by flow cytometry; 5) in vitro chromosome doubling of haploids; and 6) acclimation and selfing of selected lines. Codominant markers and flow cytometry confirmed the gametophytic origin of plants regenerated by parthenogenesis, since all homozygous lines were haploids. No spontaneous doubled haploid plants were rescued. Chromosome doubling of haploid plants was accomplished by an in vitro treatment with 500 μm colchicine. Rescue of diploid or chimeric plants was shown by flow cytometry, prior to their acclimation and planting in the greenhouse. Selfing of colchicine-treated haploid plants allowed for the perpetuation by seed of homozygous lines. The high rate of seed set, 90% of the lines produced seed, facilitated the recovery of inbred lines. Despite some limiting factors, parthenogenesis is routinely used in a cucumber-breeding program to achieve complete homozygosity in one generation. Breeding for new commercial hybrid cultivars will be accelerated. DHLs are ideal resources for genomic analyses.

Strategy for improvement of doubled haploid production in maize

2005 ◽  
Vol 53 (2) ◽  
pp. 177-182
Author(s):  
B. Barnabás ◽  
T. Spitkó ◽  
K. Jäger ◽  
J. Pintér ◽  
L. C. Marton
Keyword(s):  
Doubled Haploid ◽  
Doubled Haploid Line ◽  
Breeding Strategy ◽  
Breeding Value ◽  
Line Production ◽  
Hybrid Plants ◽  
Heterosis Effect ◽  
Anther Cultures ◽  
Chinese Germplasm

In the present study the applicability of a self-constructed doubled haploid line (DH 105) in the in vitro breeding of maize was evaluated. This line, which contained only 50% exotic (Chinese) germplasm, could be used to transmit in vitro androgenic ability into non-responsive breeding materials. F1 hybrids resulting from single crosses between the moderately responsive line DH 105 and recalcitrant genotypes with high breeding value showed a considerable heterosis effect in their androgenic responses. Most of the hybrids had favourable morphological and agronomic characters on the basis of “per se” evaluation. The data of the experiments showed that these F1 hybrid plants could be successfully used as anther donors, since numerous fertile DH plants were developed from their anther cultures. By the use of this in vitro breeding strategy the genetic variability can be widened and the effectiveness of inbred line production might be improved.

scholarly journals Inheritance of Race-Specific Resistance to Xanthomonas campestris pv. campestris in Brassica Genomes

2002 ◽  
Vol 92 (10) ◽  
pp. 1134-1141 ◽  
Author(s):  
J. G. Vicente ◽  
J. D. Taylor ◽  
A. G. Sharpe ◽  
I. A. P. Parkin ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Xanthomonas Campestris ◽  
Fragment Length Polymorphism ◽  
Specific Resistance ◽  
Doubled Haploid Line ◽  
B Genome ◽  
Brassica Spp ◽  
A Genome ◽  
Race 4 ◽  
Xanthomonas Campestris Pv Campestris

The inheritance of resistance to three Xanthomonas campestris pv. campestris races was studied in crosses between resistant and susceptible lines of Brassica oleracea (C genome), B. carinata (BC genome), and B. napus (AC genome). Resistance to race 3 in the B. oleracea doubled haploid line BOH 85c and in PI 436606 was controlled by a single dominant locus (Xca3). Resistance to races 1 and 3 in the B. oleracea line Badger Inbred-16 was quantitative and recessive. Strong resistance to races 1 and 4 was controlled by a single dominant locus (Xca1) in the B. carinata line PI 199947. This resistance probably originates from the B genome. Resistance to race 4 in three B. napus lines, cv. Cobra, the rapid cycling line CrGC5, and the doubled haploid line N-o-1, was controlled by a single dominant locus (Xca4). A set of doubled haploid lines, selected from a population used previously to develop a restriction fragment length polymorphism map, was used to map this locus. Xca4 was positioned on linkage group N5 of the B. napus A genome, indicating that this resistance originated from B. rapa. Xca4 is the first major locus to be mapped that controls race-specific resistance to X. campestris pv. campestris in Brassica spp.

scholarly journals AAC Oriental 200 oriental mustard

2018 ◽  
Vol 98 (4) ◽  
pp. 985-987
Author(s):  
Bifang Cheng ◽  
Tiina Bundrock ◽  
David J. Williams
Keyword(s):  
Doubled Haploid ◽  
Rust Resistance ◽  
Microspore Culture ◽  
Doubled Haploid Line ◽  
Western Canada ◽  
White Rust ◽  
Hybrid Plants ◽  
Oriental Mustard

AAC Oriental 200 is a doubled-haploid line. It was produced via microspore culture from the F1 hybrid plants resulting from a cross between the oriental mustard cultivars Cutlass and Forge. AAC Oriental 200 has a higher (7%) yield than the check cultivar Cutlass and similar levels of blackleg and white rust resistance. AAC Oriental 200 is well adapted to all mustard growing areas of western Canada.

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