scholarly journals Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism

2016 ◽  
Vol 14 (9) ◽  
pp. 1883-1898 ◽  
Author(s):  
Per Hofvander ◽  
Till Ischebeck ◽  
Helle Turesson ◽  
Sandeep K. Kushwaha ◽  
Ivo Feussner ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Carbohydrate Metabolism ◽  
Membrane Lipids ◽  
Central Carbohydrate Metabolism

scholarly journals Impaired chloroplast positioning affects photosynthetic capacity and regulation of the central carbohydrate metabolism during cold acclimation

2020 ◽  
Author(s):  
Anastasia Kitashova ◽  
Katja Schneider ◽  
Lisa Fürtauer ◽  
Laura Schröder ◽  
Tim Scheibenbogen ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Cold Acclimation ◽  
Metabolic Regulation ◽  
Environmental Changes ◽  
Higher Plants ◽  
Biomass Accumulation ◽  
Sucrose Phosphate Synthase ◽  
Low Light ◽  
Reduced Rate ◽  
Central Carbohydrate Metabolism

AbstractPhotosynthesis and carbohydrate metabolism of higher plants need to be tightly regulated to prevent tissue damage during environmental changes. The intracellular position of chloroplasts changes due to a changing light regime. Chloroplast avoidance and accumulation response under high and low light, respectively, are well known phenomena, and deficiency of chloroplast movement has been shown to result in photodamage and reduced biomass accumulation. Yet, effects of chloroplast positioning on underlying metabolic regulation are less well understood. Here, we analysed photosynthesis together with metabolites and enzyme activities of the central carbohydrate metabolism during cold acclimation of the chloroplast unusual positioning 1 (chup1) mutant of Arabidopsis thaliana. We compared cold acclimation under ambient and low light and found that maximum quantum yield of PSII was significantly lower in chup1 than in Col-0 under both conditions. Our findings indicated that net CO2 assimilation in chup1 is rather limited by biochemistry than by photochemistry. Further, cold-induced dynamics of sucrose phosphate synthase differed significantly between both genotypes. Together with a reduced rate of sucrose cycling derived from kinetic model simulations our study provides evidence for a central role of chloroplast positioning for photosynthetic and metabolic acclimation to low temperature.

scholarly journals DNA Microarray Analysis of Central Carbohydrate Metabolism: Glycolytic/Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum Thermoproteus tenax

2008 ◽  
Vol 190 (6) ◽  
pp. 2231-2238 ◽  
Author(s):  
Melanie Zaparty ◽  
Alexander Zaigler ◽  
Claudia Stamme ◽  
Jörg Soppa ◽  
Reinhard Hensel ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Dna Microarray ◽  
Transcript Level ◽  
Open Reading Frames ◽  
Transcriptional Analysis ◽  
Heterotrophic Growth ◽  
Thermoproteus Tenax ◽  
Central Carbohydrate Metabolism ◽  
Reading Frames

ABSTRACT In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.

Changes in flux pattern of the central carbohydrate metabolism during kernel development in maize

2005 ◽  
Vol 66 (22) ◽  
pp. 2632-2642 ◽  
Author(s):  
Christian Ettenhuber ◽  
Gertraud Spielbauer ◽  
Lilla Margl ◽  
L. Curtis Hannah ◽  
Alfons Gierl ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Kernel Development ◽  
Central Carbohydrate Metabolism

scholarly journals Engineering of Primary Carbohydrate Metabolism for Increased Production of Actinorhodin in Streptomyces coelicolor

2006 ◽  
Vol 72 (11) ◽  
pp. 7132-7139 ◽  
Author(s):  
Yong-Gu Ryu ◽  
Michael J. Butler ◽  
Keith F. Chater ◽  
Kye Joon Lee
Keyword(s):  
Carbohydrate Metabolism ◽  
Carbon Metabolism ◽  
Carbon Flux ◽  
Streptomyces Coelicolor ◽  
Central Carbon Metabolism ◽  
Acetyl Coenzyme A ◽  
Central Carbon ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Acetyl Coenzyme A Carboxylase ◽  
Central Carbohydrate Metabolism

ABSTRACT The objectives of the current studies were to determine the roles of key enzymes in central carbon metabolism in the context of increased production of antibiotics in Streptomyces coelicolor. Genes for glucose-6-phosphate dehydrogenase and phosphoglucomutase (Pgm) were deleted and those for the acetyl coenzyme A carboxylase (ACCase) were overexpressed. Under the conditions tested, glucose-6-phosphate dehydrogenase encoded by zwf2 plays a more important role than that encoded by zwf1 in determining the carbon flux to actinorhodin (Act), while the function of Pgm encoded by SCO7443 is not clearly understood. The pgm-deleted mutant unexpectedly produced abundant glycogen but was impaired in Act production, the exact reverse of what had been anticipated. Overexpression of the ACCase resulted in more rapid utilization of glucose and sharply increased the efficiency of its conversion to Act. From the current experiments, it is concluded that carbon storage metabolism plays a significant role in precursor supply for Act production and that manipulation of central carbohydrate metabolism can lead to an increased production of Act in S. coelicolor.

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