Improving cold storage and processing traits in potato through targeted gene knockout

2015 ◽  
Vol 14 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Benjamin M. Clasen ◽  
Thomas J. Stoddard ◽  
Song Luo ◽  
Zachary L. Demorest ◽  
Jin Li ◽  
...  
Keyword(s):  
Cold Storage ◽  
Gene Knockout ◽  
Targeted Gene Knockout

A Targeted Gene Knockout in Drosophila

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1307-1312 ◽  
Author(s):  
Yikang S Rong ◽  
Kent G Golic
Keyword(s):  
Homologous Recombination ◽  
Genetic Linkage ◽  
Gene Knockout ◽  
Single Gene ◽  
Marker Gene ◽  
Chromosome X ◽  
Gene Modification ◽  
Dna Fragment ◽  
Endogenous Locus ◽  
Targeted Gene Knockout

Abstract We previously described a method for targeted homologous recombination at the yellow gene of Drosophila melanogaster. Because only a single gene was targeted, further work was required to show whether the method could be extended to become generally useful for gene modification in Drosophila. We have now used this method to produce a knockout of the autosomal pugilist gene by homologous recombination between the endogenous locus and a 2.5-kb DNA fragment. This was accomplished solely by tracking the altered genetic linkage of an arbitrary marker gene as the targeting DNA moved from chromosome X or 2 to chromosome 3. The results indicate that this method of homologous recombination is likely to be generally useful for Drosophila gene targeting.

scholarly journals Transient Retrovirus-Based CRISPR/Cas9 All-in-One Particles for Efficient, Targeted Gene Knockout

2018 ◽  
Vol 13 ◽  
pp. 256-274 ◽  
Author(s):  
Yvonne Knopp ◽  
Franziska K. Geis ◽  
Dirk Heckl ◽  
Stefan Horn ◽  
Thomas Neumann ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Targeted Gene Knockout

scholarly journals Direct delivery of adenoviral CRISPR/Cas9 vector into the blastoderm for generation of targeted gene knockout in quail

2019 ◽  
Vol 116 (27) ◽  
pp. 13288-13292 ◽  
Author(s):  
Joonbum Lee ◽  
Jisi Ma ◽  
Kichoon Lee
Keyword(s):  
Gene Knockout ◽  
Primordial Germ Cells ◽  
Avian Species ◽  
Poultry Production ◽  
Cell Stage ◽  
Potential Applications ◽  
Direct Delivery ◽  
Gene Knockouts ◽  
Targeted Gene Knockout

Zygotes at the 1-cell stage have been genetically modified by microinjecting the CRISPR/Cas9 components for the generation of targeted gene knockout in mammals. In the avian species, genetic modification of the zygote is difficult because its unique reproductive system limits the accessibility of the zygote at the 1-cell stage. To date, only a few CRISPR/Cas9-mediated gene knockouts have been reported using the chicken as a model among avian species, which requires 3 major processes: isolation and culture of primordial germ cells (PGCs), modification of the genome of PGCs in vitro, and injection of the PGCs into the extraembryonic blood vessel at the early embryonic stages when endogenous PGCs migrate through circulation to the genital ridge. In the present study, the adenoviral CRISPR/Cas9 vector was directly injected into the quail blastoderm in newly laid eggs. The resulting chimeras generated offspring with targeted mutations in the melanophilin (MLPH) gene, which is involved in melanosome transportation and feather pigmentation.MLPHhomozygous mutant quail exhibited gray plumage, whereasMLPHheterozygous mutants and wild-type quail exhibited dark brown plumage. In addition, the adenoviral vector was not integrated into the genome of knockout quail, and no mutations were detected in potential off-target regions. This method of generating genome-edited poultry is expected to accelerate avian research and has potential applications for developing superior genetic lines for poultry production in the industry.

scholarly journals Targeted gene knockout in chickens mediated by TALENs

2014 ◽  
Vol 111 (35) ◽  
pp. 12716-12721 ◽  
Author(s):  
T. S. Park ◽  
H. J. Lee ◽  
K. H. Kim ◽  
J.-S. Kim ◽  
J. Y. Han
Keyword(s):  
Gene Knockout ◽  
Targeted Gene Knockout

scholarly journals Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

2011 ◽  
Vol 39 (14) ◽  
pp. 6315-6325 ◽  
Author(s):  
Ting Li ◽  
Sheng Huang ◽  
Xuefeng Zhao ◽  
David A. Wright ◽  
Susan Carpenter ◽  
...  
Keyword(s):  
Gene Knockout ◽  
Gene Replacement ◽  
Tal Effector ◽  
Tal Effector Nucleases ◽  
Targeted Gene Knockout
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