scholarly journals Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

2014 ◽  
Vol 12 (7) ◽  
pp. 934-940 ◽  
Author(s):  
William Haun ◽  
Andrew Coffman ◽  
Benjamin M. Clasen ◽  
Zachary L. Demorest ◽  
Anita Lowy ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Soybean Oil ◽  
Gene Family ◽  
Oil Quality ◽  
Fatty Acid Desaturase ◽  
Targeted Mutagenesis ◽  
Fatty Acid Desaturase 2

Insights into the novel members of the FAD2 gene family involved in high-oleate fluxes in peanut

Genome ◽  
2015 ◽  
Vol 58 (8) ◽  
pp. 375-383 ◽  
Author(s):  
Yun Wang ◽  
Xingguo Zhang ◽  
Yongli Zhao ◽  
C.S. Prakash ◽  
Guohao He ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Gene Family ◽  
Gene Networks ◽  
Genetic Manipulation ◽  
Oil Quality ◽  
Fatty Acid Desaturase ◽  
Sequence Comparisons ◽  
Developing Seeds ◽  
Fad2 Gene

The FAD2 gene family is functionally responsible for the conversion of oleic acid to linoleic acid in oilseed plants. Multiple members of the FAD gene are known to occur in several oilseed species. In this study, six novel full-length cDNA sequences (named as AhFAD2-1, -2, -3, -4, -5, and -6) were identified in peanut (Arachis hypogaea L.), an analysis of which revealed open reading frames of 379, 383, 394, or 442 amino acids. Sequence comparisons showed that AhFAD2-1 and AhFAD2-2 shared 76% identity, while AhFAD2-2, -3, and -4 displayed highly significant homology. There was only 27% identity overlap between the microsomal ω-6 fatty acid desaturase and the chloroplast ω-6 fatty acid desaturase encoded by AhFAD2-1, -2, -3, -4, and AhFAD2-5, -6, respectively. The phylogeny tree of FAD2 transcripts showed five major groups, and AhFAD2-1 was clearly separated from other groups. Analysis of AhFAD2-1 and AhFAD2-2 transcript distribution in different peanut tissues showed that the AhFAD2-1 gene showed upward of a 70-fold increase in expression of fatty acid than the AhFAD2-2 gene in peanut developing seeds, while the AhFAD2-2 gene expressed most abundantly in peanut flowers. Because the AhFAD2-1 gene played a major role in the conversion of oleic to linoleic acid during seed development, the identification of this novel member in this study would facilitate the further genetic manipulation of peanut oil quality. The implications of overall results also suggest that there may be more candidate genes controlling levels of oleate acid in developing seeds. Results also may be due to the presence of complex gene networks controlling the fluxes between the endoplasmic reticulum and the chloroplast within the peanut cells.

Novel polymorphisms in Stearoyl‐CoA Desaturase (SCD) and Fatty Acid Desaturase 2 (FADS2) in channel catfish ( Ictalurus punctatus, Rafinesque, 1818 )

2021 ◽  
Author(s):  
Oliva Mendoza‐Pacheco ◽  
Gaspar Manuel Parra‐Bracamonte ◽  
Xochitl Fabiola De la Rosa‐Reyna ◽  
Ana María Sifuentes‐Rincón ◽  
Isidro Otoniel Montelongo‐Alfaro ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Fatty Acid Desaturase ◽  
Stearoyl Coa Desaturase ◽  
Fatty Acid Desaturase 2

scholarly journals Soybean Oil-Quality Variants Identified by Large-Scale Mutagenesis

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Karen Hudson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Soybean Oil ◽  
Large Scale ◽  
Oil Quality ◽  
Soybean Seed ◽  
Subsequent Generation ◽  
Oil Composition ◽  
Fatty Acid Profiling ◽  
Wide Range

To identify genetic variation for fatty acid composition in mature soybean seeds, 4566 M3generation seed samples from a chemically mutagenized population were subjected to fatty acid profiling by gas chromatography. In the population, a wide range of variation in the content for each of the five major fatty acids was observed. Seventy-nine lines were identified which contained significantly high or low levels of one of the five major soybean fatty acids. These lines were advanced to the subsequent generation. Of the 79 lines showing a variant fatty acid profile in the M3, 52 showed clear heritability for the oil composition in the seeds of the subsequent generation. These lines are likely to represent 52 distinct genetic mutations. These mutants may represent new loci involved in the determination of soybean seed oil content or could be new isolates or alleles of previously identified genetic variants for soybean oil composition.

Gene expression of stearoyl-ACP desaturase and Δ12 fatty acid desaturase 2 is modulated during seed development of flax (Linum usitatissimum)

Lipids ◽  
2006 ◽  
Vol 41 (7) ◽  
pp. 705-712 ◽  
Author(s):  
Bourlaye Fofana ◽  
Sylvie Cloutier ◽  
Scott Duguid ◽  
Jacqueline Ching ◽  
Chris Rampitsch
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Seed Development ◽  
Linum Usitatissimum ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturase 2
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