Molecular allergy diagnosis: A potential tool for the assessment of severity of grass pollen‐induced rhinitis in children

2019 ◽  
Vol 30 (8) ◽  
pp. 852-855 ◽  
Author(s):  
Nikolaos Douladiris ◽  
Viktoria Garib ◽  
Konstantina Piskou ◽  
Margit Focke‐Tejkl ◽  
Rudolf Valenta ◽  
...  
Keyword(s):  
Grass Pollen ◽  
Allergy Diagnosis ◽  
Molecular Allergy ◽  
Potential Tool

scholarly journals FAQS on when to use Molecular Allergy Diagnosis (MD) in clinical practice

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
O Luengo ◽  
M Labrador-Horrillo
Keyword(s):  
Clinical Practice ◽  
Molecular Biology ◽  
Cross Reactivity ◽  
Practical Approach ◽  
Allergy Diagnosis ◽  
The Core ◽  
Great Progress ◽  
Molecular Allergy ◽  
The Subject

In the last decades there has been a great progress in the field of molecular biology allowing the study of the sensitization to individual allergenic components of an allergenic source, a practice that has been termed Molecular Allergy Diagnosis (MD) or Component Resolved Diagnosis (CRD). The purpose of the present review is to offer the clinician a practical approach to the use of MD by answering frequently asked questions among physicians on how MD can help us improve allergy diagnosis in our daily clinical practice. The article is divided in three sections. First, a brief review on the importance for the clinician to know the main allergens of the different allergenic sources, their structure and their in vitro cross-reactivity before approaching MD (section A). Secondly the core of the review on the usefulness of MD in clinical practice (section B) answering FAQS on the subject, and finally a section (C) on the interpretation and integration of MD with the rest of available tools for allergy diagnosis.

Molecular allergy diagnosis for the clinical characterization of asthma

2015 ◽  
Vol 15 (6) ◽  
pp. 789-799 ◽  
Author(s):  
Marina Sastre-Ibañez ◽  
Joaquín Sastre
Keyword(s):  
Allergy Diagnosis ◽  
Molecular Allergy

scholarly journals From Allergen Molecules to Molecular Immunotherapy of Nut Allergy: A Hard Nut to Crack

2021 ◽  
Vol 12 ◽  
Author(s):  
Verena Fuhrmann ◽  
Huey-Jy Huang ◽  
Aysegul Akarsu ◽  
Igor Shilovskiy ◽  
Olga Elisyutina ◽  
...  
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Class I ◽  
Food Allergens ◽  
Allergy Diagnosis ◽  
Allergen Specific Immunotherapy ◽  
Tree Nuts ◽  
Carbohydrate Epitopes ◽  
Molecular Allergy ◽  
Life Threatening

Peanuts and tree nuts are two of the most common elicitors of immunoglobulin E (IgE)-mediated food allergy. Nut allergy is frequently associated with systemic reactions and can lead to potentially life-threatening respiratory and circulatory symptoms. Furthermore, nut allergy usually persists throughout life. Whether sensitized patients exhibit severe and life-threatening reactions (e.g., anaphylaxis), mild and/or local reactions (e.g., pollen-food allergy syndrome) or no relevant symptoms depends much on IgE recognition of digestion-resistant class I food allergens, IgE cross-reactivity of class II food allergens with respiratory allergens and clinically not relevant plant-derived carbohydrate epitopes, respectively. Accordingly, molecular allergy diagnosis based on the measurement of allergen-specific IgE levels to allergen molecules provides important information in addition to provocation testing in the diagnosis of food allergy. Molecular allergy diagnosis helps identifying the genuinely sensitizing nuts, it determines IgE sensitization to class I and II food allergen molecules and hence provides a basis for personalized forms of treatment such as precise prescription of diet and allergen-specific immunotherapy (AIT). Currently available forms of nut-specific AIT are based only on allergen extracts, have been mainly developed for peanut but not for other nuts and, unlike AIT for respiratory allergies which utilize often subcutaneous administration, are given preferentially by the oral route. Here we review prevalence of allergy to peanut and tree nuts in different populations of the world, summarize knowledge regarding the involved nut allergen molecules and current AIT approaches for nut allergy. We argue that nut-specific AIT may benefit from molecular subcutaneous AIT (SCIT) approaches but identify also possible hurdles for such an approach and explain why molecular SCIT may be a hard nut to crack.

scholarly journals Molecular allergy diagnosis: Status anno 2015

2015 ◽  
Vol 444 ◽  
pp. 54-61 ◽  
Author(s):  
A.L. Van Gasse ◽  
E.A. Mangodt ◽  
M. Faber ◽  
V. Sabato ◽  
C.H. Bridts ◽  
...  
Keyword(s):  
Allergy Diagnosis ◽  
Molecular Allergy

scholarly journals Development of a protein microarray-based diagnostic chip mimicking the skin prick test for allergy diagnosis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marina Kalli ◽  
Andrew Blok ◽  
Long Jiang ◽  
Nichola Starr ◽  
Marcos J. C. Alcocer ◽  
...  
Keyword(s):  
Protein Stability ◽  
Grass Pollen ◽  
Cell Activation ◽  
Leukemia Cell ◽  
Protein Microarrays ◽  
Pollen Extract ◽  
Allergy Diagnosis ◽  
Timothy Grass ◽  
Timothy Grass Pollen ◽  
Coated Surfaces

Abstract Protein microarrays have been successfully used for detection of allergen-specific IgE in patient sera. Here, we demonstrate proof-of-concept of a solid-phase technique coupling the high-throughput potential of protein microarrays with the biologically relevant readout provided by IgE reporter cells, creating a novel allergic sensitization detection system. Three proteins (κ-casein, timothy grass pollen extract, polyclonal anti-human IgE) were printed onto three different polymer-coated surfaces (aldehyde-, epoxy- and NHS ester-coated). ToF–SIMs analysis was performed to assess printed protein stability and retention during washing steps. NFAT-DsRed rat basophil leukemia cell attachment and retention during washing steps was assessed after treatment with various extracellular matrix proteins. NFAT-DsRed IgE reporter cells were sensitized with serum of an allergic donor, incubated on the printed slides, and cell activation determined using a microarray laser scanner. NFAT DsRed IgE reporter cell binding was significantly increased on all polymer surfaces after incubation with fibronectin and vitronectin, but not collagen or laminin. All surfaces supported printed protein stability during washing procedure, with epoxy- and NHS ester-coated surfaces showing best protein retention. Cell activation was significantly higher in NHS ester-coated slides after timothy grass pollen extract stimulation appearing a suitable substrate for further development of an automated allergy diagnosis system.

Molecular allergy diagnosis: we need to become more knowledgeable

2012 ◽  
Vol 108 (6) ◽  
pp. 387 ◽  
Author(s):  
Giovanni Melioli ◽  
Giorgio Walter Canonica
Keyword(s):  
Allergy Diagnosis ◽  
Molecular Allergy

Molecular diagnosis and precision medicine in allergy management

2016 ◽  
Vol 54 (11) ◽  
Author(s):  
Anna Maria Riccio ◽  
Laura De Ferrari ◽  
Alessandra Chiappori ◽  
Sabina Ledda ◽  
Giovanni Passalacqua ◽  
...  
Keyword(s):  
Precision Medicine ◽  
Allergen Immunotherapy ◽  
Structural Model ◽  
Allergy Diagnosis ◽  
Medical Decisions ◽  
Allergen Components ◽  
Allergy Diagnostics ◽  
Molecular Allergy ◽  
Detailed Level ◽  
Appropriate Prescription

AbstractPrecision medicine (PM) can be defined as a structural model aimed at customizing healthcare, with medical decisions/products tailored on an individual patient at a highly detailed level. In this sense, allergy diagnostics based on molecular allergen components allows to accurately define the patient’s IgE repertoire. The availability of highly specialized singleplexed and multiplexed platforms support allergists with an advanced diagnostic armamentarium. The therapeutic intervention, driven by the standard diagnostic approach, but further supported by these innovative tools may result, for instance, in a more appropriate prescription of allergen immunotherapy (AIT). Also, the phenotyping of patients, which may have relevant effects on the treatment strategy, could be take advantage by the molecular allergy diagnosis.

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