Porphyromonas gingivalis indirectly elicits intestinal inflammation by altering the gut microbiota and disrupting epithelial barrier function through IL9‐producing CD4 + T cells

2021 ◽  
Author(s):  
Jiho Sohn ◽  
Lu Li ◽  
Lixia Zhang ◽  
Prasad R. Settem ◽  
Kiyonobu Honma ◽  
...  
Keyword(s):  
T Cells ◽  
Gut Microbiota ◽  
Porphyromonas Gingivalis ◽  
Barrier Function ◽  
Cd4 T Cells ◽  
Intestinal Inflammation ◽  
Epithelial Barrier ◽  
Epithelial Barrier Function

scholarly journals Notch-1 Signaling Regulates Intestinal Epithelial Barrier Function, Through Interaction With CD4+ T Cells, in Mice and Humans

2011 ◽  
Vol 140 (2) ◽  
pp. 550-559 ◽  
Author(s):  
Stephanie Dahan ◽  
Keren M. Rabinowitz ◽  
Andrea P. Martin ◽  
M. Cecilia Berin ◽  
Jay C. Unkeless ◽  
...  
Keyword(s):  
T Cells ◽  
Barrier Function ◽  
Cd4 T Cells ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Notch 1 ◽  
Intestinal Epithelial Barrier ◽  
Epithelial Barrier Function

119 Bacterial Proteases Contribute to the Development of Chronic Intestinal Inflammation By Impairing Epithelial Barrier Function

2009 ◽  
Vol 136 (5) ◽  
pp. A-21-A-22
Author(s):  
Natalie Steck ◽  
Micha Hoffmann ◽  
Carrie M. Hew Ferstl ◽  
Sandra C. Kim ◽  
Bo Liu ◽  
...  
Keyword(s):  
Barrier Function ◽  
Intestinal Inflammation ◽  
Epithelial Barrier ◽  
Epithelial Barrier Function ◽  
Chronic Intestinal Inflammation

scholarly journals Anthocyanin Improves Glucose Homeostasis in Obese Mice via Regulation of Intestinal Microbiota and Barrier Function

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 491-491
Author(s):  
Hongliang Xue ◽  
Wenhua Ling
Keyword(s):  
Gut Microbiota ◽  
Systemic Inflammation ◽  
Glucose Homeostasis ◽  
Barrier Function ◽  
Tolerance Test ◽  
Intestinal Barrier Function ◽  
Epithelial Barrier ◽  
Oral Glucose ◽  
Epithelial Barrier Function ◽  
Funding Sources

Abstract Objectives Anthocyanin (ACN) with low bioavailability possess anti-diabetic effect, the mechanism of the action remained unclear. This study aimed to investigate whether the anti-diabetic effects of anthocyanin were related to regulation of gut microbiota and epithelial barrier function. Methods Male C57Bl/6N mice were randomly assigned into 3 groups and fed chow, high fat/high sugar diet (HFHS, 45 kcal% fat, 17 kcal% sucrose) or HFHS + 0.4% anthocyanin for 8 weeks. Furthermore, a separate cohort of HFHS-fed C57Bl/6N mice were oral gavage with fecal microbiota from HFHS, HFHS + ACN and heat-killed fecal microbial from HFHS + ACN-fed donors (HK-ACN) for 4 weeks, The composition of gut microbiota, systemic inflammation, glucose homeostasis and intestinal barrier function were determined. Results The area under the curve (AUC) of the oral glucose tolerance test (GTT) and insulin tolerance test (ITT) in HFHS-fed mice were 61% and 79% higher than those in Chow-fed mice (P < 0.05). ACN supplementation improved HFHS-induced glucose intolerance in the GTT (P < 0.05) and restore insulin sensitivity in the ITT (P < 0.05). ACN supplementation also attenuated HFHS-induced systemic inflammation, intestinal permeability and metabolic endotoxemia (P < 0.05). In addition, ACN administration increased expression of epithelial barrier function genes ZO-1, Occludin and decreased inducible NO-synthase (iNOS) protein levels in intestine of HFHS-fed mice. Gut microbiota analysis showed that ACN greatly altered gut microbiota of HFHS-fed mice. Transplantation of the gut microbiota from ACN-fed mice, but not HFHS-fed or HK-ACN-fed mice, significantly improved intestinal epithelial barrier function, systemic inflammation and glucose homeostasis. Conclusions The present study demonstrated that ACN improves HFHS-induced impairment of glucose metabolism by regulating the gut microbiota and epithelial barrier functions. Funding Sources No funding sources to report.

scholarly journals Anthocyanin Improves Glucose Homeostasis in Obese Mice via Beneficial Regulation of Intestinal Microbiota and Barrier Function (OR34-08-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Hongliang Xue ◽  
Xu Chen ◽  
Ke Chen ◽  
Yiran You ◽  
Huihui Xu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Systemic Inflammation ◽  
Glucose Homeostasis ◽  
Gut Microbiome ◽  
Barrier Function ◽  
Intestinal Barrier Function ◽  
Epithelial Barrier ◽  
Rrna Gene ◽  
Epithelial Barrier Function

Abstract Objectives Anthocyanin (ACN) is a natural polyphenol with anti-diabetic effects. However, intact anthocyanin has low bioavailability and largely arrives unmetabolized in the colon, its mechanisms of action remain unclear. The intestinal microbiota dysbiosis and leaky gut contribute to the development of diet-induced type 2 diabetes. Therefore, we aim to investigate whether the anti-diabetic effects of anthocyanin were related to changes in the gut microbiota and epithelial barrier function. Methods Male C57Bl/6 N mice were randomly assigned into 4 groups and pair-fed either a chow or high fat/high sugar diet (HFHS, 45 kcal% fat, 17 kcal% sucrose) +/- 1.0% anthocyanin for 8 weeks. Indices of systemic inflammation, parameters of glucose homeostasis and intestinal barrier function were determined. The composition of the gut microbiota was assessed by analysing 16S rRNA gene sequences with Illumina pyrosequencing. To ascertain the involvement of the gut microbiota in the anti-diabetic effects of anthocyanin. A separate cohort of HFHS-fed C57Bl/6 N mice were oral gavage administered with fecal microbiota from chow-fed donors, HFHS-fed donors, HFHS + ACN-fed donors and heat-killed fecal microbial from HFHS + ACN-fed donors (HK-ACN) for 8 weeks, followed by the same examination. Results Relative to vehicle controls, ACN ingestion attenuated several effects of HFHS feeding, including glucose intolerance, insulin resistance and serum inflammatory markers. ACN ingestion reduced intestinal permeability and metabolic endotoxemia. These beneficial effects of ACN were associated with increased expression of genes involved in epithelial barrier function (ZO-1, occludin) and decreased inducible NO-synthase (iNOS) protein levels in ileum and colon of HFHS-fed mice. Gut microbiota analysis revealed that ACN ingestion induced profound alterations in the gut microbiome of HFHS-fed mice. Transplantation of the gut microbiome from ACN-fed mice, but not HFHS-fed or HK-ACN-fed mice, was sufficient to recapitulate the improvement in intestinal epithelial barrier function, systemic inflammation and glucose homeostasis observed with oral ACN treatment. Conclusions These findings indicate that ACN-mediated changes in the gut microbiota and epithelial barrier function may play a predominant role in the mechanism of action of anthocyanin. Funding Sources The State Key Program of National Natural Science Foundation of China [grant number 81730090].

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