Role of HIF‐2α/NF‐κB pathway in mechanical stress‐induced temporomandibular joint osteoarthritis

Oral Diseases ◽  
2021 ◽  
Author(s):  
Wen Li ◽  
Na Wu ◽  
Junming Wang ◽  
Yingnan Wang ◽  
Mengjie Wu ◽  
...  
Keyword(s):  
Mechanical Stress ◽  
Temporomandibular Joint ◽  
Temporomandibular Joint Osteoarthritis

scholarly journals Role of Hyaluronic Acid in the Homeostasis and Therapeutics of Temporomandibular Joint Osteoarthritis

2017 ◽  
Vol 35 (3) ◽  
pp. 870-876 ◽  
Author(s):  
Veronica Iturriaga ◽  
Bélgica Vásquez ◽  
Carlos Manterola ◽  
Mariano del Sol
Keyword(s):  
Hyaluronic Acid ◽  
Temporomandibular Joint ◽  
Temporomandibular Joint Osteoarthritis

scholarly journals Role of the SDF-1/CXCR4 signaling pathway in cartilage and subchondral bone in temporomandibular joint osteoarthritis induced by overloaded functional orthopedics in rats

2020 ◽  
Author(s):  
jing yang ◽  
Yazhen Li ◽  
Ying Liu ◽  
Qiang Zhang ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Signaling Pathway ◽  
Subchondral Bone ◽  
Cartilage Damage ◽  
Mandibular Advancement ◽  
Micro Computed Tomography ◽  
Cxcr4 Signaling ◽  
Skeletal Class Ii ◽  
Temporomandibular Joint Osteoarthritis

Abstract Objectives: To: (i) use a mandibular-advancement appliance in rats to investigate the role of the stromal cell-derived factor/ CXC receptor 4 (SDF-1/CXCR4) signaling pathway in temporomandibular joint osteoarthritis (TMJ OA) induced by overloaded functional orthopedics (OFO); (ii) provide a cellular and molecular basis for efficacious treatment of skeletal class-II malocclusion and avoidance of TMJ OA.Method: Male Sprague–Dawley rats (6 weeks) were divided randomly into control+normal saline (NS), EXP+ADM3100 (SDF-1 antagonist), EXP+NS, and control+ADM3100 groups. Changes in articular cartilage and subchondral bone after TMJ OA in these four groups were observed by hematoxylin and eosin (H&E), Immunofluorescence double staining (IDS), Safranin-O staining, immunohistochemical (IHC) staining, real-time polymerase chain reaction, and micro-computed tomography at 2, 4, and 8 weeks.Results: OFO led to increased expression of SDF-1, CXCR4, and matrix metalloproteinase (MMP)13 and decreased expression of collagen II. The thickness of the hypertrophic cartilage layer was reduced at 4 weeks in the EXP+NS group, and damage to subchondral bone was observed at 2 weeks. Using ADM3100 to inhibit SDF-1 signaling could attenuate expression of MMP13, cartilage damage, and osteoblast differentiation. IDS showed that the areas of expression of SDF-1 and OSX in subchondral bone overlapped.Conclusions: Overloaded functional orthopedics (OFO) induced TMJ-OA. The destruction of subchondral bone in TMJ OA caused by OFO occurred before damage to cartilage. SDF-1/CXCR4 may induce the osteogenic differentiation and cause cartilage degradation in TMJ OA caused by OFO.

scholarly journals The immunoregulatory role of p21 in the development of the temporomandibular joint‐osteoarthritis

2021 ◽  
Author(s):  
Tsendsuren Khurel‐Ochir ◽  
Takashi Izawa ◽  
Akihiko Iwasa ◽  
Fumiya Kano ◽  
Akihito Yamamoto ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Temporomandibular Joint Osteoarthritis

scholarly journals Role of the SDF-1/CXCR4 signaling pathway in cartilage and subchondral bone in temporomandibular joint osteoarthritis induced by overloaded functional orthopedics in rats

2020 ◽  
Author(s):  
jing yang ◽  
Yazhen Li ◽  
Ying Liu ◽  
Qiang Zhang ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Signaling Pathway ◽  
Subchondral Bone ◽  
Cartilage Damage ◽  
Mandibular Advancement ◽  
Micro Computed Tomography ◽  
Cxcr4 Signaling ◽  
Skeletal Class Ii ◽  
Temporomandibular Joint Osteoarthritis

Abstract Objectives To: (i) use a mandibular-advancement appliance in rats to investigate the role of the stromal cell-derived factor/ CXC receptor 4 (SDF-1/CXCR4) signaling pathway in temporomandibular joint osteoarthritis (TMJ OA) induced by overloaded functional orthopedics (OFO); (ii) provide a cellular and molecular basis for efficacious treatment of skeletal class-II malocclusion and avoidance of TMJ OA.Method: Male Sprague–Dawley rats (6 weeks) were divided randomly into control + normal saline (NS), EXP + ADM3100 (SDF-1 antagonist), EXP + NS, and control + ADM3100 groups. Changes in articular cartilage and subchondral bone after TMJ OA in these four groups were observed by hematoxylin and eosin (H&E), Immunofluorescence double staining (IDS), Safranin-O staining, immunohistochemical (IHC) staining, real-time polymerase chain reaction, and micro-computed tomography at 2, 4, and 8 weeks.Results OFO led to increased expression of SDF-1, CXCR4, and matrix metalloproteinase (MMP)13 and decreased expression of collagen II. The thickness of the hypertrophic cartilage layer was reduced at 4 weeks in the EXP + NS group, and damage to subchondral bone was observed at 2 weeks. Using ADM3100 to inhibit SDF-1 signaling could attenuate expression of MMP13, cartilage damage, and osteoblast differentiation. IDS showed that the areas of expression of SDF-1 and OSX in subchondral bone overlapped.Conclusions The destruction of subchondral bone in TMJ OA caused by OFO occurred before damage to cartilage. An increase in expression of the SDF-1/CXCR4 signaling pathway in TMJ OA induced by OFO enabled osteoblasts in subchondral bone to up-regulate expression of SDF-1.

Exploring the Biological Protective Role of Carotenoids by Raman Spectroscopy: Mechanical Stress of Cells

2019 ◽  
Vol 64 (1-2) ◽  
pp. 75-82
Author(s):  
F. Nekvapil ◽  
◽  
Cs. Müller Molnár ◽  
S. Tomšić ◽  
S. Cintă Pinzaru ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Mechanical Stress ◽  
Protective Role

Stages of the formation of temporary and permanent occlusion and the impact of early tooth extraction on the dentition state. Literature review

2019 ◽  
pp. 22-24
Author(s):  
G.A. Murachueva ◽  
I.M. Rasulov ◽  
S.G. Gusenov
Keyword(s):  
Literature Review ◽  
Temporomandibular Joint ◽  
Tooth Extraction ◽  
Physiological State ◽  
Review Of The Literature ◽  
Full Development ◽  
Permanent Occlusion ◽  
The Impact

A review of the literature on the stages of the formation of temporary and permanent occlusion has been performed. This stages play an important role not only for the full development of the maxillofacial apparatus, temporomandibular joint, but also the whole organism. The role of early tooth extraction in the formation of the physiological state of the dentoalveolar system is considered. The conclusion is drawn about the need for a deeper study of this problem in the structure of general dental morbidity.

scholarly journals Temporomandibular Joint Osteoarthritis: Regenerative Treatment by a Stem Cell Containing Advanced Therapy Medicinal Product (ATMP)—An In Vivo Animal Trial

2021 ◽  
Vol 22 (1) ◽  
pp. 443
Author(s):  
Robert Köhnke ◽  
Marcus Oliver Ahlers ◽  
Moritz Alexander Birkelbach ◽  
Florian Ewald ◽  
Michael Krueger ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Medicinal Product ◽  
Temporomandibular Joint ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Advanced Therapy Medicinal Product ◽  
Animal Trial ◽  
Advanced Therapy ◽  
Temporomandibular Joint Osteoarthritis

Temporomandibular joint osteoarthritis (TMJ-OA) is a chronic degenerative disease that is often characterized by progressive impairment of the temporomandibular functional unit. The aim of this randomized controlled animal trial was a comparative analysis regarding the chondroregenerative potency of intra-articular stem/stromal cell therapy. Four weeks after combined mechanical and biochemical osteoarthritis induction in 28 rabbits, therapy was initiated by a single intra-articular injection, randomized into the following groups: Group 1: AB Serum (ABS); Group 2: Hyaluronic acid (HA); Group 3: Mesenchymal stromal cells (STx.); Group 4: Mesenchymal stromal cells in hyaluronic acid (HA + STx.). After another 4 weeks, the animals were euthanized, followed by histological examination of the removed joints. The histological analysis showed a significant increase in cartilage thickness in the stromal cell treated groups (HA + STx. vs. ABS, p = 0.028; HA + ST.x vs. HA, p = 0.042; STx. vs. ABS, p = 0.036). Scanning electron microscopy detected a similar heterogeneity of mineralization and tissue porosity in the subchondral zone in all groups. The single intra-articular injection of a stem cell containing, GMP-compliant advanced therapy medicinal product for the treatment of iatrogen induced osteoarthritis of the temporomandibular joint shows a chondroregenerative effect.

Sign in / Sign up

Export Citation Format

Share Document