Inhibitory effects of circulating natural autoantibodies to CD47‐derived peptides on OSCC cells

Oral Diseases ◽  
2021 ◽  
Author(s):  
Xiu Liu ◽  
Chao Liang ◽  
Qingyong Meng ◽  
Yi Qu ◽  
Ziyi He ◽  
...  
Keyword(s):  
Inhibitory Effects ◽  
Natural Autoantibodies

scholarly journals Inhibitory Effects of Circulating Natural Autoantibodies to CD47-derived Peptides on Oral Squamous Cell Carcinoma Cells

2020 ◽  
Author(s):  
Xiu Liu ◽  
Qingyong Meng ◽  
Ziyi He ◽  
Rui Dong ◽  
Ying Hu
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Immune Surveillance ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibitory Effects ◽  
Natural Autoantibodies ◽  
Cluster Of Differentiation

Abstract Background Natural autoantibodies serve as an important anti-tumorigenic system due to its immune surveillance function. The present study aims to investigate whether circulating natural IgG autoantibodies against cluster of differentiation 47 (CD47) could inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells. Methods The expression of 14 tumor-targeted genes in three OSCC cell lines was analyzed with quantitative real-time PCR. The in-house enzyme-linked immunosorbent assay (ELISA) was then performed to detect plasma IgG antibodies against CD47. Three OSCC cell lines were treated with 20% human plasma positive and negative for anti-CD47 IgG, respectively, followed by analysis of cell proliferation, apoptosis and invasion/metastasis. Results CD 47 showed highest expression among all 14 genes detected in three OSCC cell lines. Plasma anti-CD47 IgG significantly inhibited the viability of all three OSCC cell lines. The proportions of apoptotic cells were remarkably higher in OSCC cells treated with anti-CD47 IgG-positive plasma than those treated with IgG-negative plasma. Furthermore, the cell invasion/metastasis was attenuated evidently with the attribution of plasma anti-CD47 IgG. Conclusions Natural autoantibodies against CD47 may be a potential target for OSCC immunotherapy.

scholarly journals Inhibitory Effects of Circulating Natural Autoantibodies to CD47-derived Peptides on Oral Squamous Cell Carcinoma Cells

2020 ◽  
Author(s):  
Xiu Liu ◽  
Qingyong Meng ◽  
Ziyi He ◽  
Rui Dong ◽  
Ying Hu
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Immune Surveillance ◽  
Carcinoma Cells ◽  
Inhibitory Effects ◽  
Natural Autoantibodies ◽  
Cluster Of Differentiation

Abstract Natural autoantibodies serve as an important anti-tumorigenic system due to its immune surveillance function. The present study aims to investigate whether circulating natural IgG autoantibodies against cluster of differentiation 47 (CD47) could suppress the proliferation of oral squamous cell carcinoma (OSCC) cells. Three OSCC cell lines were used for this study. The expression of fourteen tumor related genes including CD47 were tested. OSCC cells were grown, respectively, with 20% human plasma positive and negative for anti-CD47 IgG. Cell proliferation, apoptosis and invasion/metastasis were examined. The results showed that CD47 presented highest expression among all 14 genes detected in OSCC cells. Plasma anti-CD47 IgG significantly inhibited the viability of all three OSCC cell lines. The proportions of apoptotic cells were remarkably higher in OSCC cells treated with anti-CD47 IgG-positive plasma than those treated with IgG-negative plasma. Furthermore, the cell invasion/metastasis was attenuated evidently with the attribution of plasma anti-CD47 IgG. In conclusion, natural autoantibodies against CD47 may be a potential target for OSCC immunotherapy.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

Inhibitory Effects of Transposed Letter Neighbours During Silent Sentence Reading

2014 ◽  
Author(s):  
Ascension Pagan ◽  
Hazel Blythe ◽  
Kevin Paterson ◽  
Simon Liversedge
Keyword(s):  
Inhibitory Effects ◽  
Sentence Reading
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