Gene and immunohistochemical expression of HIF‐1α, GLUT‐1, FASN, and adipophilin in carcinoma ex pleomorphic adenoma development

Oral Diseases ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 1190-1199 ◽  
Author(s):  
João Figueira Scarini ◽  
Larissa Fernandes Rosa ◽  
Reydson Alcides de Lima Souza ◽  
Erika Said Abu Egal ◽  
Alfio José Tincani ◽  
...  
Keyword(s):  
Pleomorphic Adenoma ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Immunohistochemical Expression ◽  
Glut 1

Immunohistochemical expression of mammaglobin in salivary duct carcinomas de novo and salivary duct carcinoma ex pleomorphic adenoma

2019 ◽  
Vol 92 ◽  
pp. 59-66 ◽  
Author(s):  
Ciro Dantas Soares ◽  
Thayná Melo de Lima Morais ◽  
Roman Carlos ◽  
Manoela Domingues Martins ◽  
Oslei Paes de Almeida ◽  
...  
Keyword(s):  
Pleomorphic Adenoma ◽  
De Novo ◽  
Salivary Duct Carcinoma ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Immunohistochemical Expression ◽  
Salivary Duct ◽  
Duct Carcinoma

Analysis of the Expression of Genes Related to Lipidic and Glycogenic Metabolism in Pleomorphic Adenomas and Carcinomas Ex Pleomorphic Adenomas

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S135-S136
Author(s):  
Erika Egal ◽  
Joao Scarini ◽  
Larissa Fernandes ◽  
Welligton Sabino ◽  
Reydson Souza ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Pleomorphic Adenoma ◽  
Malignant Transformation ◽  
Fatty Acid Synthase ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Glut 1 ◽  
Significant Difference ◽  
Pleomorphic Adenomas

Abstract Introduction The carcinoma ex pleomorphic adenoma (CXPA) arises from a pleomorphic adenoma (PA) and is supposed to gain lipogenesis and glycogenesis during its malignant transformation. Increased lipogenesis is a characteristic of cancer cells, consisting of increased fatty acid synthesis by the enzyme fatty acid synthase (FASN) and by the accumulation of cytoplasmic lipid droplets, which express adipophilin. The increase in glycolytic metabolism has been associated with the activity of glycolytic enzymes and glucose transporters (GLUTs), whose expression is induced by HIF-1 (inducing factor of hypoxia 1). Objectives To analyze the gene expression of FASN, adipophilin, HIF-1α, and GLUT-1 in PA and CXPA samples. Methodology Gene expression analysis of the FASN, adipophilin, HIF1-α, and GLUT-1 genes was performed by the real-time PCR (qPCR) method. Fourteen cases of PA and 14 cases of CXAP were evaluated. Results FASN, adipophilin, HIF-1α, and GLUT-1 were more expressed in CXAP than in AP, although there was no significant difference between levels of adipophilin expression in CXPA and PA. Conclusion Increased expression of FASN, HIF1-α, and GLUT-1 in CXAP may be associated with malignant transformation of PA, while additional studies are needed to understand the role of adipophilin in PA carcinogenesis. Moreover, this is the first study to investigate the role of these genes in the malignant transformation of PA.

IMMUNOHISTOCHEMICAL EXPRESSION OF METABOLIC MARKERS IN PLEOMORPHIC ADENOMA AND CARCINOMA EX PLEOMORPHIC ADENOMA

2020 ◽  
Vol 130 (3) ◽  
pp. e265-e266
Author(s):  
JOHN LENNON SILVA CUNHA ◽  
THAYNá MELO DE LIMA MORAIS ◽  
CINTHIA BARDáLEZ LóPEZ DE CÁCERES ◽  
OSLEI PAES DE ALMEIDA ◽  
ROMáN CARLOS ◽  
...  
Keyword(s):  
Pleomorphic Adenoma ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Immunohistochemical Expression ◽  
Metabolic Markers

scholarly journals Immunohistochemical Expression of p53 in Pleomorphic Adenoma and Carcinoma Ex Pleomorphic Adenoma

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Bassel Tarakji ◽  
Omar Kujan ◽  
Mohammad Z. Nassani
Keyword(s):  
Salivary Gland ◽  
Parotid Gland ◽  
Pleomorphic Adenoma ◽  
Diagnostic Marker ◽  
Suppressor Gene ◽  
Undifferentiated Carcinoma ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Immunohistochemical Expression ◽  
Histologic Types ◽  
Tumor Suppressor Gene P53

Context. Immunohistochemical stains for p53 are used as a diagnostic marker associated with malignancy in several histologic types of salivary gland tumors. This marker may be useful in differentiating pleomorphic adenoma (PA) from carcinoma ex pleomorphic adenoma (CPA), as these tumors are often difficult to distinguish on the basis of morphology alone.Objective. to evaluate whatever inactivation of tumor suppressor gene (p53) increases with the tumor progression from normal salivary tissue to PA and eventually CPA.Design. Paraffin blocks of 29 cases of PA, which were surrounded by normal parotid gland, and 27 cases of carcinoma ex pleomorphic adenoma were retrieved and validated. In all cases of carcinoma ex pleomorphic adenoma, a PA “ghost” was identified, and the malignant element was either undifferentiated carcinoma or adenocarcinoma.Results. The results showed negative nuclear expression of P53 in normal parotid gland. Nuclear P53 was expressed strongly in 6/29 (20.7%) pleomorphic salivary adenoma and 10/27 (37%) carcinoma ex pleomorphic adenoma.Conclusion. Our data suggest that inactivation of p53 may play an important role in the evolution of pleomorphic salivary adenoma and carcinoma ex pleomorphic adenoma.

FASN AND GLUT-1 EXPRESSION IN THE PLEOMORPHIC ADENOMA AND CARCINOMA EX-PLEOMORPHIC ADENOMA

2020 ◽  
Vol 129 (1) ◽  
pp. e153
Author(s):  
JOÃO FIGUEIRA SCARINI ◽  
LOUYSE VIZOTTO CARVALHO ◽  
LARISSA FERNANDES ◽  
DÉBORA CAMPANELLA BASTOS ◽  
ALBINA ALTEMANI ◽  
...  
Keyword(s):  
Pleomorphic Adenoma ◽  
Carcinoma Ex Pleomorphic Adenoma ◽  
Glut 1
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