Matrilineal empowers wheat pollen with haploid induction potency via triggering post‐mitosis ROS activity

2022 ◽  
Author(s):  
Guoliang Sun ◽  
Shuaifeng Geng ◽  
Hongjie Zhang ◽  
Meiling Jia ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Haploid Induction

Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
R. K Khulbe ◽  
A. Pattanayak ◽  
Lakshmi Kant ◽  
G. S. Bisht ◽  
M. C. Pant ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Sweet Corn ◽  
Haploid Induction ◽  
Maize Breeding ◽  
Line Production ◽  
Haploid Inducer ◽  
Breeding Programmes ◽  
Source Populations ◽  
Developmental Phases

The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.

scholarly journals Haploid induction by a maize cenh3 null mutant

2021 ◽  
Vol 7 (4) ◽  
pp. eabe2299 ◽  
Author(s):  
Na Wang ◽  
Jonathan I. Gent ◽  
R. Kelly Dawe
Keyword(s):  
Histone H3 ◽  
Null Mutation ◽  
Null Mutant ◽  
Wild Type ◽  
Haploid Induction ◽  
Cell Divisions ◽  
Gamete Formation ◽  
Simplified Method ◽  
Single Cross ◽  
Subsequent Loss

The production of haploids is an important first step in creating many new plant varieties. One approach used in Arabidopsis involves crossing plants expressing different forms of centromeric histone H3 (CENP-A/CENH3) and subsequent loss of genome with weaker centromeres. However, the method has been ineffective in crop plants. Here, we describe a greatly simplified method based on crossing maize lines that are heterozygous for a cenh3 null mutation. Crossing +/cenh3 to wild-type plants in both directions yielded haploid progeny. Genome elimination was determined by the cenh3 genotype of the gametophyte, suggesting that centromere failure is caused by CENH3 dilution during the postmeiotic cell divisions that precede gamete formation. The cenh3 haploid inducer works as a vigorous hybrid and can be transferred to other lines in a single cross, making it versatile for a variety of applications.

In Vivo Haploid Induction in Maize: Comparison of Different Testing Regimes for Measuring Haploid Induction Rates

Crop Science ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 1127-1135 ◽  
Author(s):  
Albrecht E. Melchinger ◽  
Pedro Correa Brauner ◽  
Juliane Böhm ◽  
Wolfgang Schipprack
Keyword(s):  
Haploid Induction

scholarly journals Genetic Approaches to Enhance Multiple Stress Tolerance in Maize

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1760
Author(s):  
Nenad Malenica ◽  
Jasenka Antunović Dunić ◽  
Lovro Vukadinović ◽  
Vera Cesar ◽  
Domagoj Šimić
Keyword(s):  
Stress Tolerance ◽  
Stress Responses ◽  
Association Studies ◽  
Metal Exposure ◽  
Genome Wide Association Studies ◽  
Haploid Induction ◽  
Multiple Stress ◽  
Resistant Varieties ◽  
Maize Varieties ◽  
Multiple Stress Tolerance

The multiple-stress effects on plant physiology and gene expression are being intensively studied lately, primarily in model plants such as Arabidopsis, where the effects of six stressors have simultaneously been documented. In maize, double and triple stress responses are obtaining more attention, such as simultaneous drought and heat or heavy metal exposure, or drought in combination with insect and fungal infestation. To keep up with these challenges, maize natural variation and genetic engineering are exploited. On one hand, quantitative trait loci (QTL) associated with multiple-stress tolerance are being identified by molecular breeding and genome-wide association studies (GWAS), which then could be utilized for future breeding programs of more resilient maize varieties. On the other hand, transgenic approaches in maize have already resulted in the creation of many commercial double or triple stress resistant varieties, predominantly weed-tolerant/insect-resistant and, additionally, also drought-resistant varieties. It is expected that first generation gene-editing techniques, as well as recently developed base and prime editing applications, in combination with the routine haploid induction in maize, will pave the way to pyramiding more stress tolerant alleles in elite lines/varieties on time.

scholarly journals Extension of thein vivohaploid induction system from maize to wheat

2019 ◽  
Author(s):  
Chenxu Liu ◽  
Yu Zhong ◽  
Xiaolong Qi ◽  
Ming Chen ◽  
Zongkai Liu ◽  
...  
Keyword(s):  
Pollen Viability ◽  
Haploid Induction ◽  
Male Sterile ◽  
Crop Breeding ◽  
Crop Species ◽  
Ploidy Levels ◽  
New Approach ◽  
Knock Out ◽  
Haploid Production

AbstractDoubled haploid breeding technology has been one of the most important techniques for accelerating crop breeding. In compare toin vivohaploid induction in maize, which is efficient and background independent, wheat haploid production by interspecific hybridization pollinated with maize is influenced by genetic background and requires rescue of young embryos. Here, we analyzed the homologues of maize haploid induction geneMTL/ZmPLA1/NLDin several crop species systematically, the homologues are highly conserved in sorghum, millet and wheat etc. Since wheat is a very important polyploidy crop, as a proof of concept, we demonstrated that thein vivohaploid induction method could be extended from diploid maize to hexaploid wheat by knocking out the wheat homologues (TaPLAs). Result showed that double knock-out mutation could trigger wheat haploid induction at ~ 2%-3%, accompanied by 30% - 60% seed setting rate. The performance of haploid wheat individual showed shorter plant, narrower leaves and male sterile. Our results also revealed that knockout ofTaPLA-A andTaPLA-D do not affect pollen viability. This study not only confirmed the function of the induction gene and explored a new approach for haploid production in wheat, but also provided an example that thein vivohaploid induction could be applied in more crop species with different ploidy levels. Furthermore, by combining with gene editing, it would be a fast and powerful platform for traits improvement in polyploidy crops breeding.

scholarly journals The Genetic Basis of Haploid Induction in Maize Identified with a Novel Genome-Wide Association Method

Genetics ◽  
2016 ◽  
Vol 202 (4) ◽  
pp. 1267-1276 ◽  
Author(s):  
Haixiao Hu ◽  
Tobias A. Schrag ◽  
Regina Peis ◽  
Sandra Unterseer ◽  
Wolfgang Schipprack ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Genome Wide Association ◽  
Haploid Induction ◽  
Genome Wide ◽  
Association Method

Comparative Embryogenesis in Angiosperms: Activation and Patterning of Embryonic Cell Lineages

2021 ◽  
Vol 72 (1) ◽  
Author(s):  
Thomas Dresselhaus ◽  
Gerd Jürgens
Keyword(s):  
Cell Fate ◽  
Embryo Development ◽  
Flowering Plants ◽  
Egg Cell ◽  
Annual Review ◽  
Publication Date ◽  
Zygotic Embryogenesis ◽  
Haploid Induction ◽  
Cell Lineages ◽  
Basic Body

Following fertilization in flowering plants (angiosperms), egg and sperm cells unite to form the zygote, which generates an entire new organism through a process called embryogenesis. In this review, we provide a comparative perspective on early zygotic embryogenesis in flowering plants by using the Poaceae maize and rice as monocot grass and crop models as well as Arabidopsis as a eudicot model of the Brassicaceae family. Beginning with the activation of the egg cell, we summarize and discuss the process of maternal-to-zygotic transition in plants, also taking recent work on parthenogenesis and haploid induction into consideration. Aspects like imprinting, which is mainly associated with endosperm development and somatic embryogenesis, are not considered. Controversial findings about the timing of zygotic genome activation as well as maternal versus paternal contribution to zygote and early embryo development are highlighted. The establishment of zygotic polarity, asymmetric division, and apical and basal cell lineages represents another chapter in which we also examine and compare the role of major signaling pathways, cell fate genes, and hormones in early embryogenesis. Except for the model Arabidopsis, little is known about embryo patterning and the establishment of the basic body plan in angiosperms. Using available in situ hybridization, RNA-sequencing, and marker data, we try to compare how and when stem cell niches are established. Finally, evolutionary aspects of plant embryo development are discussed. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

scholarly journals The genotypic response towards haploid induction in Turkish onion (Allium cepa L.) germplasm

2020 ◽  
Vol 48 (3) ◽  
pp. 1176-1184
Author(s):  
Faika YARALI KARAKAN
Keyword(s):  
Flow Cytometry ◽  
Allium Cepa ◽  
Ms Medium ◽  
Haploid Induction ◽  
Flower Buds ◽  
Plant Survival ◽  
Survival Percentage ◽  
Induction Rate ◽  
Allium Cepa L ◽  
Medium Flow

Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).

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