GDSL lipase occluded stomatal pore 1 is required for wax biosynthesis and stomatal cuticular ledge formation

2020 ◽  
Vol 228 (6) ◽  
pp. 1880-1896 ◽  
Author(s):  
Jing Tang ◽  
Xianpeng Yang ◽  
Chuanlei Xiao ◽  
Jiaying Li ◽  
Yongqiang Chen ◽  
...  
Keyword(s):  
Gdsl Lipase

scholarly journals The GDSL Lipase MHZ11 Modulates Ethylene Signaling in Rice Roots

2020 ◽  
Vol 32 (5) ◽  
pp. 1626-1643 ◽  
Author(s):  
He Zhao ◽  
Biao Ma ◽  
Kai-Xuan Duan ◽  
Xin-Kai Li ◽  
Xiang Lu ◽  
...  
Keyword(s):  
Ethylene Signaling ◽  
Rice Roots ◽  
Gdsl Lipase

scholarly journals Integrated Metabolomic and Transcriptomic Analysis to Characterize Cutin Biosynthesis between Low- and High-Cutin Genotypes of Capsicum chinense Jacq

2020 ◽  
Vol 21 (4) ◽  
pp. 1397 ◽  
Author(s):  
Purushothaman Natarajan ◽  
Tolulope Abodunrin Akinmoju ◽  
Padma Nimmakayala ◽  
Carlos Lopez-Ortiz ◽  
Marleny Garcia-Lozano ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Biosynthetic Pathway ◽  
Molecular Mechanisms ◽  
Extreme Temperature ◽  
Transcriptome Profiling ◽  
Transcriptomic Analysis ◽  
Biotic And Abiotic Stresses ◽  
Habanero Pepper ◽  
And Storage ◽  
Gdsl Lipase

Habanero peppers constantly face biotic and abiotic stresses such as pathogen/pest infections, extreme temperature, drought and UV radiation. In addition, the fruit cutin lipid composition plays an important role in post-harvest water loss rates, which in turn causes shriveling and reduced fruit quality and storage. In this study, we integrated metabolome and transcriptome profiling pertaining to cutin in two habanero genotypes: PI 224448 and PI 257145. The fruits were selected by the waxy or glossy phenotype on their surfaces. Metabolomics analysis showed a significant variation in cutin composition, with about 6-fold higher cutin in PI 257145 than PI 224448. It also revealed that 10,16-dihydroxy hexadecanoic acid is the most abundant monomer in PI 257145. Transcriptomic analysis of high-cutin PI 257145 and low-cutin PI 224448 resulted in the identification of 2703 statistically significant differentially expressed genes, including 1693 genes upregulated and 1010 downregulated in high-cutin PI 257145. Genes and transcription factors such as GDSL lipase, glycerol-3 phosphate acyltransferase 6, long-chain acyltransferase 2, cytochrome P450 86A/77A, SHN1, ANL2 and HDG1 highly contributed to the high cutin content in PI 257145. We predicted a putative cutin biosynthetic pathway for habanero peppers based on deep transcriptome analysis. This is the first study of the transcriptome and metabolome pertaining to cutin in habanero peppers. These analyses improve our knowledge of the molecular mechanisms regulating the accumulation of cutin in habanero pepper fruits. These resources can be built on for developing cultivars with high cutin content that show resistance to biotic and abiotic stresses with superior postharvest appearance.

scholarly journals Identification of Genes Preferentially Expressed in Stomatal Guard Cells of Arabidopsis thaliana and Involvement of the Aluminum-Activated Malate Transporter 6 Vacuolar Malate Channel in Stomatal Opening

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenxiu Ye ◽  
Shota Koya ◽  
Yuki Hayashi ◽  
Huimin Jiang ◽  
Takaya Oishi ◽  
...  
Keyword(s):  
Guard Cells ◽  
Stomatal Opening ◽  
Stomatal Movement ◽  
Null Mutant ◽  
Reverse Transcription Pcr ◽  
Public Resources ◽  
Stomatal Guard Cells ◽  
Signaling Components ◽  
Malate Transporter ◽  
Gdsl Lipase

Stomatal guard cells (GCs) are highly specialized cells that respond to various stimuli, such as blue light (BL) and abscisic acid, for the regulation of stomatal aperture. Many signaling components that are involved in the stomatal movement are preferentially expressed in GCs. In this study, we identified four new such genes in addition to an aluminum-activated malate transporter, ALMT6, and GDSL lipase, Occlusion of Stomatal Pore 1 (OSP1), based on the expression analysis using public resources, reverse transcription PCR, and promoter-driven β-glucuronidase assays. Some null mutants of GC-specific genes evidenced altered stomatal movement. We further investigated the role played by ALMT6, a vacuolar malate channel, in stomatal opening. Epidermal strips from an ALMT6-null mutant exhibited defective stomatal opening induced by BL and fusicoccin, a strong plasma membrane H+-ATPase activator. The deficiency was enhanced when the assay buffer [Cl–] was low, suggesting that malate and/or Cl– facilitate efficient opening. The results indicate that the GC-specific genes are frequently involved in stomatal movement. Further detailed analyses of the hitherto uncharacterized GC-specific genes will provide new insights into stomatal regulation.

scholarly journals A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Sissi Miguel ◽  
Guillaume Legrand ◽  
Léonor Duriot ◽  
Marianne Delporte ◽  
Barbara Menin ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Ipomoea Batatas ◽  
Efficient Production ◽  
Coffee Bean ◽  
The Road ◽  
Dicaffeoylquinic Acid ◽  
Low Efficiency ◽  
Green Coffee Bean Extract ◽  
Gdsl Lipase

AbstractThe synthesis of 3,5-dicaffeoylquinic acid (3,5-DiCQA) has attracted the interest of many researchers for more than 30 years. Recently, enzymes belonging to the BAHD acyltransferase family were shown to mediate its synthesis, albeit with notably low efficiency. In this study, a new enzyme belonging to the GDSL lipase-like family was identified and proven to be able to transform chlorogenic acid (5-O-caffeoylquinic acid, 5-CQA, CGA) in 3,5-DiCQA with a conversion rate of more than 60%. The enzyme has been produced in different expression systems but has only been shown to be active when transiently synthesized in Nicotiana benthamiana or stably expressed in Pichia pastoris. The synthesis of the molecule could be performed in vitro but also by a bioconversion approach beginning from pure 5-CQA or from green coffee bean extract, thereby paving the road for producing it on an industrial scale.

scholarly journals RMS2 Encoding a GDSL Lipase Mediates Lipid Homeostasis in Anthers to Determine Rice Male Fertility

2020 ◽  
Vol 182 (4) ◽  
pp. 2047-2064 ◽  
Author(s):  
Juan Zhao ◽  
Tuan Long ◽  
Yifeng Wang ◽  
Xiaohong Tong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Male Fertility ◽  
Lipid Homeostasis ◽  
Gdsl Lipase

Molecular Cloning and Functional Analysis of a GDSL Lipase Gene fromGossypium hirsutumL.

2013 ◽  
Vol 39 (7) ◽  
pp. 1164
Author(s):  
Xiang-Chao TONG ◽  
Li-Man WANG ◽  
Wen-Jing HU ◽  
Xue-Ying ZHANG ◽  
Tian-Zhen ZHANG ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Lipase Gene ◽  
Gdsl Lipase

scholarly journals GDSL lipase 1 regulates ethylene signaling and ethylene-associated systemic immunity inArabidopsis

FEBS Letters ◽  
2014 ◽  
Vol 588 (9) ◽  
pp. 1652-1658 ◽  
Author(s):  
Hye Gi Kim ◽  
Sun Jae Kwon ◽  
Young Jin Jang ◽  
Joo Hee Chung ◽  
Myung Hee Nam ◽  
...  
Keyword(s):  
Ethylene Signaling ◽  
Systemic Immunity ◽  
Gdsl Lipase

GDSL lipase-like 1 regulates systemic resistance associated with ethylene signaling in Arabidopsis

2009 ◽  
Vol 58 (2) ◽  
pp. 235-245 ◽  
Author(s):  
Sun Jae Kwon ◽  
Hak Chul Jin ◽  
Soohyun Lee ◽  
Myung Hee Nam ◽  
Joo Hee Chung ◽  
...  
Keyword(s):  
Systemic Resistance ◽  
Ethylene Signaling ◽  
Gdsl Lipase
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