scholarly journals Auxin‐induced actin cytoskeleton rearrangements require AUX1

2020 ◽  
Vol 226 (2) ◽  
pp. 441-459 ◽  
Author(s):  
Ruthie S. Arieti ◽  
Christopher J. Staiger
Keyword(s):  
Actin Cytoskeleton ◽  
Cytoskeleton Rearrangements

scholarly journals Schip1 Is a Novel Podocyte Foot Process Protein that Mediates Actin Cytoskeleton Rearrangements and Forms a Complex with Nherf2 and Ezrin

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122067 ◽  
Author(s):  
Ljubica Perisic ◽  
Patricia Q. Rodriguez ◽  
Kjell Hultenby ◽  
Ying Sun ◽  
Mark Lal ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Foot Process ◽  
Cytoskeleton Rearrangements

scholarly journals Role of γ-Adducin in Actin Cytoskeleton Rearrangements in Podocyte Pathophysiology

2020 ◽  
Author(s):  
Wenjun Gao ◽  
Yedan Liu ◽  
Letao Fan ◽  
Baoying Zheng ◽  
Joshua R. Jefferson ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Vascular Function ◽  
Renal Cortex ◽  
Transgenic Rats ◽  
Focal Adhesion Proteins ◽  
Foot Process ◽  
And Function ◽  
Cytoskeleton Rearrangements ◽  
Renal Vascular

We recently reported that the enhanced susceptibility to chronic kidney disease (CKD) in the FHH rat is caused, at least in part, by a mutation in γ-adducin (ADD3) that attenuates renal vascular function. The present study explored whether Add3 contributes to the modulation of podocyte structure and function using FHH and FHH.Add3 transgenic rats. The expression of ADD3 on the membrane of primary podocytes isolated from FHH was reduced compared with FHH.Add3 transgenic rats. We found that F-actin nets, which are typically localized in the lamellipodia, replaced unbranched stress fibers in conditionally immortalized mouse podocytes transfected with Add3 DsiRNA and primary podocytes isolated from FHH rats. There were increased F/G-actin ratio and expression of the Arp2/3 complexes throughout FHH podocytes in association with reduced synaptopodin and RhoA but enhanced Rac1 and CDC42 expression in the renal cortex, glomeruli and podocytes of FHH rats. The expression of Nephrin at the slit diaphragm and the levels of focal adhesion proteins ITGA3 and ITGB1 were decreased in the glomeruli of FHH rats. Cell migration was enhanced and adhesion was reduced in podocytes of FHH rats, as well as in immortalized mouse podocyte transfected with Add3 DsiRNA. Mean arterial pressures were similar in FHH and FHH.Add3 transgenic rats at 16-week of age; however, FHH rats exhibited enhanced proteinuria associated with podocyte foot process effacement. These results demonstrate that reduced ADD3 function in FHH rats alters baseline podocyte pathophysiology by rearrangement of the actin cytoskeleton at the onset of proteinuria in young animals.

scholarly journals The Role of Nod Factor Substituents in Actin Cytoskeleton Rearrangements in Phaseolus vulgaris

2003 ◽  
Vol 16 (4) ◽  
pp. 326-334 ◽  
Author(s):  
Luis Cárdenas ◽  
Jane E. Thomas-Oates ◽  
Noreide Nava ◽  
Isabel M. López-Lara ◽  
Peter K. Hepler ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Root Hair ◽  
Terminal Residue ◽  
Root Hair Cell ◽  
Acetyl Group ◽  
Cytoskeletal Rearrangements ◽  
Cytoskeleton Rearrangements ◽  
Hair Curling ◽  
Root Hair Curling

In order to define the symbiotic role of some of the chemical substituents in the Rhizobium etli Nod factors (NFs), we purified Nod metabolites secreted by the SM25 strain, which carries most of the nodulation genes, and SM17 with an insertion in nodS. These NFs were analyzed for their capabilities to induce root hair curling and cytoskeletal rearrangements. The NFs secreted by strain SM17 lack the carbamoyl and methyl substituents on the nonreducing terminal residue and an acetyl moiety on the fucosyl residue on the reducing-terminal residue as determined by mass spectrometry. We have reported previously that the root hair cell actin cytoskeleton from bean responds with a rapid fragmentation of the actin bundles within 5 min of NF exposure, and also is accompanied by increases in the apical influxes and intracellular calcium levels. In this article, we report that methyl-bearing NFs are more active in inducing root hair curling and actin cytoskeleton rearrangements than nonmethylated NFs. However, the carbamoyl residue on the nonreducing terminal residue and the acetyl group at the fucosyl residue on the reducing terminal residue do not seem to have any effect on root hair curling induction or in actin cytoskeleton rearrangement.

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