scholarly journals Coupling of microRNA-directed phased small interfering RNA generation from long noncoding genes with alternative splicing and alternative polyadenylation in small RNA-mediated gene silencing

2017 ◽  
Vol 217 (4) ◽  
pp. 1535-1550 ◽  
Author(s):  
Wuqiang Ma ◽  
Chengjie Chen ◽  
Yuanlong Liu ◽  
Ming Zeng ◽  
Blake C. Meyers ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Gene Silencing ◽  
Small Rna ◽  
Small Interfering Rna ◽  
Alternative Polyadenylation ◽  
Interfering Rna

Targeted Gene Silencing by Small Interfering RNA-Based Knock-Down Technology

2004 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Bentham Science Publisher Zhang J. ◽  
Bentham Science Publisher Hua Z.C.
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Knock Down ◽  
Interfering Rna

Pre-Injection of Small Interfering RNA (siRNA) Promotes c-Jun Gene Silencing and Decreases the Survival Rate of Axotomy-Injured Spinal Motoneurons in Adult Mice

2018 ◽  
Vol 65 (3) ◽  
pp. 400-410 ◽  
Author(s):  
Ying-qin Li ◽  
Fa-huan Song ◽  
Ke Zhong ◽  
Guang-yin Yu ◽  
Prince Last Mudenda Zilundu ◽  
...  
Keyword(s):  
Survival Rate ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Spinal Motoneurons ◽  
Adult Mice ◽  
Interfering Rna

scholarly journals Carbon Dots for Efficient Small Interfering RNA Delivery and Gene Silencing in Plants

2020 ◽  
Vol 184 (2) ◽  
pp. 647-657 ◽  
Author(s):  
Steven H. Schwartz ◽  
Bill Hendrix ◽  
Paul Hoffer ◽  
Rick A. Sanders ◽  
Wei Zheng
Keyword(s):  
Gene Silencing ◽  
Carbon Dots ◽  
Small Interfering Rna ◽  
Interfering Rna

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

Small interfering RNA delivery and gene silencing using polymeric nanocarrier systems

2005 ◽  
Vol 1 (3) ◽  
pp. 299-299
Author(s):  
K. A. Howard ◽  
X. Liu ◽  
D. Oupicky ◽  
S. Zoffmann ◽  
F. Besenbacher ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Interfering Rna ◽  
Polymeric Nanocarrier

scholarly journals Intravaginal gene silencing using biodegradable polymer nanoparticles densely loaded with small-interfering RNA

2009 ◽  
Vol 8 (6) ◽  
pp. 526-533 ◽  
Author(s):  
Kim A. Woodrow ◽  
Yen Cu ◽  
Carmen J. Booth ◽  
Jennifer K. Saucier-Sawyer ◽  
Monica J. Wood ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Biodegradable Polymer ◽  
Small Interfering Rna ◽  
Polymer Nanoparticles ◽  
Interfering Rna

scholarly journals Molecular mechanisms of Dicer: endonuclease and enzymatic activity

2017 ◽  
Vol 474 (10) ◽  
pp. 1603-1618 ◽  
Author(s):  
Min-Sun Song ◽  
John J. Rossi
Keyword(s):  
Enzymatic Activity ◽  
Small Rna ◽  
Small Rnas ◽  
Small Interfering Rna ◽  
Molecular Mechanisms ◽  
Cellular Processes ◽  
Interfering Rna ◽  
Exogenous Substrates

The enzyme Dicer is best known for its role as a riboendonuclease in the small RNA pathway. In this canonical role, Dicer is a critical regulator of the biogenesis of microRNA and small interfering RNA, as well as a growing number of additional small RNAs derived from various sources. Emerging evidence demonstrates that Dicer's endonuclease role extends beyond the generation of small RNAs; it is also involved in processing additional endogenous and exogenous substrates, and is becoming increasingly implicated in regulating a variety of other cellular processes, outside of its endonuclease function. This review will describe the canonical and newly identified functions of Dicer.

scholarly journals Cryo-EM Studies of an Engineered Small Interfering RNA Nano-Ring used as a Gene Silencing Therapeutic

2013 ◽  
Vol 104 (2) ◽  
pp. 350a
Author(s):  
Alexey Y. Koyfman ◽  
Kirill A. Afonin ◽  
Mathias Viard ◽  
Wojciech K. Kasprzak ◽  
Bruce A. Shapiro ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Interfering Rna
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