scholarly journals Two proteases with caspase-3-like activity, cathepsin B and proteasome, antagonistically control ER-stress-induced programmed cell death in Arabidopsis

2017 ◽  
Vol 218 (3) ◽  
pp. 1143-1155 ◽  
Author(s):  
Yao-Min Cai ◽  
Jia Yu ◽  
Yuan Ge ◽  
Aleksandr Mironov ◽  
Patrick Gallois
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Er Stress ◽  
Cathepsin B ◽  
Caspase 3

scholarly journals Inhibition of cathepsin B by caspase-3 inhibitors blocks programmed cell death in Arabidopsis

2016 ◽  
Vol 23 (9) ◽  
pp. 1493-1501 ◽  
Author(s):  
Y Ge ◽  
Y-M Cai ◽  
L Bonneau ◽  
V Rotari ◽  
A Danon ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Enzymatic Activity ◽  
Cathepsin B ◽  
Caspase 3 ◽  
Animal Cells ◽  
Biotic And Abiotic Stresses ◽  
Triple Mutant ◽  
Mutant Lines

Abstract Programmed cell death (PCD) is used by plants for development and survival to biotic and abiotic stresses. The role of caspases in PCD is well established in animal cells. Over the past 15 years, the importance of caspase-3-like enzymatic activity for plant PCD completion has been widely documented despite the absence of caspase orthologues. In particular, caspase-3 inhibitors blocked nearly all plant PCD tested. Here, we affinity-purified a plant caspase-3-like activity using a biotin-labelled caspase-3 inhibitor and identified Arabidopsis thaliana cathepsin B3 (AtCathB3) by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Consistent with this, recombinant AtCathB3 was found to have caspase-3-like activity and to be inhibited by caspase-3 inhibitors. AtCathepsin B triple-mutant lines showed reduced caspase-3-like enzymatic activity and reduced labelling with activity-based caspase-3 probes. Importantly, AtCathepsin B triple mutants showed a strong reduction in the PCD induced by ultraviolet (UV), oxidative stress (H2O2, methyl viologen) or endoplasmic reticulum stress. Our observations contribute to explain why caspase-3 inhibitors inhibit plant PCD and provide new tools to further plant PCD research. The fact that cathepsin B does regulate PCD in both animal and plant cells suggests that this protease may be part of an ancestral PCD pathway pre-existing the plant/animal divergence that needs further characterisation.

scholarly journals Correction to: Inhibition of cathepsin B by caspase-3 inhibitors blocks programmed cell death in Arabidopsis

2018 ◽  
Vol 25 (8) ◽  
pp. 1532-1532 ◽  
Author(s):  
Y. Ge ◽  
Y-M. Cai ◽  
L. Bonneau ◽  
V. Rotari ◽  
A. Danon ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Cathepsin B ◽  
Caspase 3

scholarly journals Cell Death and Metabolic Stress in Gymnodinium catenatum Induced by Allelopathy

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 506
Author(s):  
Leyberth José Fernández-Herrera ◽  
Christine Johanna Band-Schmidt ◽  
Tania Zenteno-Savín ◽  
Ignacio Leyva-Valencia ◽  
Claudia Judith Hernández-Guerrero ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Protein Content ◽  
Caspase 3 ◽  
Cellular Stress ◽  
Allelopathic Effect ◽  
Sod Activity ◽  
Free Media ◽  
O2 Production ◽  
Gymnodinium Catenatum

Allelopathy between phytoplankton species can promote cellular stress and programmed cell death (PCD). The raphidophyte Chattonella marina var. marina, and the dinoflagellates Margalefidinium polykrikoides and Gymnodinium impudicum have allelopathic effects on Gymnodinium catenatum; however, the physiological mechanisms are unknown. We evaluated whether the allelopathic effect promotes cellular stress and activates PCD in G. catenatum. Cultures of G. catenatum were exposed to cell-free media of C. marina var. marina, M. polykrikoides and G. impudicum. The mortality, superoxide radical (O2●−) production, thiobarbituric acid reactive substances (TBARS) levels, superoxide dismutase (SOD) activity, protein content, and caspase-3 activity were quantified. Mortality (between 57 and 79%) was registered in G. catenatum after exposure to cell-free media of the three species. The maximal O2●− production occurred with C. marina var. marina cell-free media. The highest TBARS levels and SOD activity in G. catenatum were recorded with cell-free media from G. impudicum. The highest protein content was recorded with cell-free media from M. polykrikoides. All cell-free media caused an increase in the activity of caspase-3. These results indicate that the allelopathic effect in G. catenatum promotes cell stress and caspase-3 activation, as a signal for the induction of programmed cell death.

Region of caspase-3 activation and programmed cell death in the early development of the mouse forebrain

2003 ◽  
Vol 145 (2) ◽  
pp. 241-248 ◽  
Author(s):  
Koko Urase ◽  
Yoriko Kouroku ◽  
Eriko Fujita ◽  
Takashi Momoi
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Early Development ◽  
Caspase 3

scholarly journals Persistent ER Stress Induces the Spliced Leader RNA Silencing Pathway (SLS), Leading to Programmed Cell Death in Trypanosoma brucei

2010 ◽  
Vol 6 (1) ◽  
pp. e1000731 ◽  
Author(s):  
Hanoch Goldshmidt ◽  
Devorah Matas ◽  
Anat Kabi ◽  
Shai Carmi ◽  
Ronen Hope ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Er Stress ◽  
Trypanosoma Brucei ◽  
Rna Silencing ◽  
Spliced Leader ◽  
Spliced Leader Rna
Sign in / Sign up

Export Citation Format

Share Document