scholarly journals LlFH1-mediated interaction between actin fringe and exocytic vesicles is involved in pollen tube tip growth

2017 ◽  
Vol 214 (2) ◽  
pp. 745-761 ◽  
Author(s):  
Shanwei Li ◽  
Huaijian Dong ◽  
Weike Pei ◽  
Chaonan Liu ◽  
Sha Zhang ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Tip Growth

scholarly journals Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

2021 ◽  
Vol 40 (2) ◽  
pp. 205-222
Author(s):  
Monica Scali ◽  
Alessandra Moscatelli ◽  
Luca Bini ◽  
Elisabetta Onelli ◽  
Rita Vignani ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Membrane Trafficking ◽  
Tip Growth ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Structural Features ◽  
Two Dimensional Gel Electrophoresis ◽  
Depth Analysis

AbstractPollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identifyNicotiana tabacumDifferentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

scholarly journals Ca2+-Activated Reactive Oxygen Species Production by Arabidopsis RbohH and RbohJ Is Essential for Proper Pollen Tube Tip Growth

2014 ◽  
Vol 26 (3) ◽  
pp. 1069-1080 ◽  
Author(s):  
Hidetaka Kaya ◽  
Ryo Nakajima ◽  
Megumi Iwano ◽  
Masahiro M. Kanaoka ◽  
Sachie Kimura ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Pollen Tube ◽  
Reactive Oxygen Species Production ◽  
Tip Growth ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Production

scholarly journals Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 156 ◽  
Author(s):  
Nolan Scheible ◽  
Andrew McCubbin
Keyword(s):  
Pollen Tube ◽  
Signaling Pathways ◽  
Pollen Tube Growth ◽  
Tip Growth ◽  
Holistic Approach ◽  
Pollen Tubes ◽  
Small Gtpase ◽  
Tube Growth ◽  
Concerted Action ◽  
Current State

The coordinated growth of pollen tubes through floral tissues to deliver the sperm cells to the egg and facilitate fertilization is a highly regulated process critical to the Angiosperm life cycle. Studies suggest that the concerted action of a variety of signaling pathways underlies the rapid polarized tip growth exhibited by pollen tubes. Ca2+ and small GTPase-mediated pathways have emerged as major players in the regulation of pollen tube growth. Evidence suggests that these two signaling pathways not only integrate with one another but also with a variety of other important signaling events. As we continue to elucidate the mechanisms involved in pollen tube growth, there is a growing importance in taking a holistic approach to studying these pathways in order to truly understand how tip growth in pollen tubes is orchestrated and maintained. This review considers our current state of knowledge of Ca2+-mediated and GTPase signaling pathways in pollen tubes, how they may intersect with one another, and other signaling pathways involved. There will be a particular focus on recent reports that have extended our understanding in these areas.

scholarly journals A Gain-of-Function Mutation of Arabidopsis Lipid Transfer Protein 5 Disturbs Pollen Tube Tip Growth and Fertilization

2009 ◽  
Vol 21 (12) ◽  
pp. 3902-3914 ◽  
Author(s):  
Keun Chae ◽  
Chris A. Kieslich ◽  
Dimitrios Morikis ◽  
Seung-Chul Kim ◽  
Elizabeth M. Lord
Keyword(s):  
Pollen Tube ◽  
Tip Growth ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Gain Of Function ◽  
Transfer Protein

Pollination Sub-Systems Distinguished by Pollen Tube Arrest after Incompatible Interspecific Crosses in Rhododendron (Ericaceae)

1982 ◽  
Vol 53 (1) ◽  
pp. 255-277
Author(s):  
ELIZABETH G. WILLIAMS ◽  
BRUCE R. KNOX ◽  
JOHN L. ROUSE
Keyword(s):  
Pollen Tube ◽  
Tip Growth ◽  
Interspecific Cross ◽  
Embryo Sac ◽  
Epifluorescence Microscopy ◽  
Interspecific Crosses ◽  
Abnormal Behaviour ◽  
Callose Deposition ◽  
Variable Diameter ◽  
Stigmatic Exudate

The cytology of compatible and interspecific incompatible pollinations has been followed in selected species of the genus Rhododendron (Ericaceae). Pollinated pistils were fixed, cleared, stained in decolourized aniline blue, and observed by epifluorescence microscopy. Ten different abnormalities of arrested pollen tube tips have been detected, including burst, tapered, swollen, coiled, spiralling, spiky and variable diameter syndromes. A series of five errors of callose deposition in incompatible tubes has also been defined. Six different regions in the pistil for expression of pollen tube arrest have been found, including the stigmatic exudate, the mucilage of the upper and lower style canal, the ovary loculus, the micropyle. There may also be abnormal behaviour after entry into the embryo sac. Both the site of pollen tube arrest within the pistil, and the error syndrome of tip growth and callose deposition anomalies, are characteristic of each interspecific cross. These results are discussed in relation to the genetic control of reproduction.

Calcium Control of Pollen Tube Tip Growth

1989 ◽  
Vol 176 (2S) ◽  
pp. 18-20 ◽  
Author(s):  
MARTIN W. STEER
Keyword(s):  
Pollen Tube ◽  
Tip Growth

scholarly journals Pollen Tube and Root-Hair Tip Growth Is Disrupted in a Mutant of Arabidopsis thaliana

1993 ◽  
Vol 103 (3) ◽  
pp. 979-985 ◽  
Author(s):  
J. Schiefelbein ◽  
M. Galway ◽  
J. Masucci ◽  
S. Ford
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Root Hair ◽  
Tip Growth

scholarly journals Exocytosis-coordinated mechanisms for tip growth underlie pollen tube growth guidance

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Nan Luo ◽  
An Yan ◽  
Gang Liu ◽  
Jingzhe Guo ◽  
Duoyan Rong ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tip Growth ◽  
Tube Growth ◽  
Growth Guidance
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