Single‐cell RNA sequencing predicts motility networks in purified human gastric interstitial cells of Cajal

2021 ◽  
Author(s):  
Daphne Foong ◽  
Liwan Liyanage ◽  
Jerry Zhou ◽  
Ali Zarrouk ◽  
Vincent Ho ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Single Cell Rna Sequencing ◽  
Cells Of Cajal

scholarly journals Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
Raúl Loera-Valencia ◽  
Xuan-Yu Wang ◽  
George Wright ◽  
Carlos Barajas-López ◽  
Jan Huizinga
Keyword(s):  
Single Cell ◽  
Interstitial Cells Of Cajal ◽  
Expression Patterns ◽  
Mrna Level ◽  
Primary Cultures ◽  
Interstitial Cells ◽  
Mrna Analysis ◽  
Cells Of Cajal ◽  
Cell Expression ◽  
The Relationship

AbstractThe interstitial cells of Cajal (ICC) drive the slow wave-associated contractions in the small intestine. A commonly used marker for these cells is c-Kit, but another marker named Ano1 was recently described. This study uses single-cell RT-PCR, qPCR and immunohistochemistry to determine if Ano1 could be reliably used as a molecular marker for ICC in single-cell mRNA analysis. Here, we report on the relationship between the expression of c-Kit and Ano1 in single ICC in culture. We observed that Ano1 is expressed in more than 60% of the collected cells, whereas c-Kit is found only in 22% of the cells (n = 18). When we stained ICC primary cultures for c-KIT and ANO1 protein, we found complete co-localization in all the preparations. We propose that this difference is due to the regulation of c-Kit mRNA in culture. This regulation gives rise to low levels of its transcript, while Ano1 is expressed more prominently in culture on day 4. We also propose that Ano1 is more suitable for single-cell expression analysis as a marker for cell identity than c-Kit at the mRNA level. We hope this evidence will help to validate and increase the success of future studies characterizing single ICC expression patterns.

Sodium current in human intestinal interstitial cells of Cajal

2003 ◽  
Vol 285 (6) ◽  
pp. G1111-G1121 ◽  
Author(s):  
Peter R. Strege ◽  
Yijun Ou ◽  
Lei Sha ◽  
Adam Rich ◽  
Simon J. Gibbons ◽  
...  
Keyword(s):  
Single Cell ◽  
Slow Wave ◽  
Interstitial Cells Of Cajal ◽  
Sodium Current ◽  
Interstitial Cells ◽  
Wave Frequency ◽  
Rt Pcr ◽  
Α Subunit ◽  
Channel Current ◽  
Cells Of Cajal

Interstitial cells of Cajal (ICC) generate the electrical slow wave required for normal gastrointestinal motility. The ionic conductances expressed in human intestinal ICC are unknown. The aim of this study was to determine expression of a Na+ current in human intestinal ICC and to determine the effects of the Na+ current on the slow wave. Visually identified, freshly dissociated, single ICC were verified by the presence of c- kit mRNA by using single-cell RT-PCR. Standard whole cell currents were recorded from patch-clamped ICC held at -100 mV between pulse protocols. A Na+ current was identified in human intestinal ICC. The current activated at -55 mV and peaked at -30 mV. Extracellular N-methyl-d-glucamine abolished and QX-314 (500 μM) blocked the Na+ current, but nifedipine and Ni2+ did not. The Na+ current was activated by shear stress. Single-cell RT-PCR detected mRNA for the Na+ α-subunit SCN5A in single human intestinal ICC. Lidocaine (200 μm) and QX-314 (500 μM) decreased slow wave frequency, and stretch increased slow wave frequency. A mechanosensitive Na+ channel current is present in human intestinal ICC and appears to play a role in the control of intestinal motor function.

Sodium current in human small intestinal interstitial cells of cajal

2001 ◽  
Vol 120 (5) ◽  
pp. A201-A201 ◽  
Author(s):  
P STREGE ◽  
A RICH ◽  
Y OU ◽  
S GIBBONS ◽  
M SARR ◽  
...  
Keyword(s):  
Interstitial Cells Of Cajal ◽  
Sodium Current ◽  
Interstitial Cells ◽  
Small Intestinal ◽  
Cells Of Cajal

41-OR: Single Cell RNA-Sequencing Reveals the Developmental Heterogeneity of Brown Adipocytes

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 41-OR
Author(s):  
FARNAZ SHAMSI ◽  
MARY PIPER ◽  
LI-LUN HO ◽  
TIAN LIAN HUANG ◽  
YU-HUA TSENG
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Brown Adipocytes ◽  
Single Cell Rna Sequencing
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