Upregulation of intestinal mucosal mast cells expressing VPAC1 in close proximity to vasoactive intestinal polypeptide in inflammatory bowel disease and murine colitis

2018 ◽  
Vol 31 (3) ◽  
pp. e13503 ◽  
Author(s):  
Maite Casado‐Bedmar ◽  
Stéphanie D. S. Heil ◽  
Pär Myrelid ◽  
Johan D. Söderholm ◽  
Åsa V. Keita
Keyword(s):  
Inflammatory Bowel Disease ◽  
Mast Cells ◽  
Vasoactive Intestinal Polypeptide ◽  
Bowel Disease ◽  
Murine Colitis ◽  
Mucosal Mast Cells ◽  
Close Proximity ◽  
Inflammatory Bowel ◽  
Intestinal Polypeptide

scholarly journals Vasoactive Intestinal Polypeptide (VIP) in the Intestinal Mucosal Nerve Fibers in Dogs with Inflammatory Bowel Disease

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1759
Author(s):  
Andrzej Rychlik ◽  
Sławomir Gonkowski ◽  
Jarosław Całka ◽  
Krystyna Makowska
Keyword(s):  
Inflammatory Bowel Disease ◽  
Nervous System ◽  
Vasoactive Intestinal Polypeptide ◽  
Bowel Disease ◽  
Disease Process ◽  
Nerve Fibers ◽  
Inflammatory Bowel ◽  
Healthy Dogs ◽  
Adaptive Reactions ◽  
Intestinal Polypeptide

Canine inflammatory bowel disease (IBD) is a group of enteropathies with nonspecific chronic symptoms and poorly understood etiology. Many aspects connected with IBD are not understood. One of them is the participation of the intestinal nervous system in the development of pathological processes. Thus, this study aimed to demonstrate changes in the density of intramucosal nerve fibers containing vasoactive intestinal polypeptide (VIP)—one of the most important intestinal nervous factors caused by the various stages of IBD development. Mucosal biopsy specimens collected from the duodenum, jejunum and descending colon of healthy dogs and dogs with varied severity of IBD were included in the experiment. The density of VIP-like immunoreactive (VIP-LI) nerves was determined by a single immunofluorescence technique and a semi-quantitative method consisting in VIP-LI fiber counts in the field of view (0.1 mm2). The obtained results indicate that IBD induces changes in the density of mucosal VIP-LI nerve fibers in the canine gastrointestinal tract. The initial decrease is followed by an increase in VIP-like immunoreactivity in successive stages of the disease. These observations show that VIP is a neuronal factor that participates in the pathological processes connected with canine IBD. The observed changes probably result from the neuroprotective and/or adaptive properties of VIP. Protective and adaptive reactions induced by inflammation aim to protect the GI tract against damage by proinflammatory factors and ensure the homeostasis in the enteric nervous system (ENS) under the conditions changed by the disease process.

scholarly journals Mucosal Mast Cells in Irritable Bowel Syndrome and Inflammatory Bowel Disease

2005 ◽  
Vol 48 (3-4) ◽  
pp. 163-164 ◽  
Author(s):  
Bilge Tunc ◽  
Levent Filik ◽  
Engin Altıntas ◽  
Nesrin Turhan ◽  
Aysel Ulker ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Mast Cell ◽  
Irritable Bowel Syndrome ◽  
Mast Cells ◽  
Bowel Disease ◽  
Mucosal Mast Cell ◽  
Cell Counts ◽  
Mucosal Mast Cells ◽  
Inflammatory Bowel ◽  
Irritable Bowel

Even though exciting progresses have been until now, further studies are necessary to clearly understand the significance of MMC. Mast cells are thought to participate in the pathogenesis of inflammatory bowel disease and irritable bowel syndrome. However, their role in the pathogenesis remains unsettled. The specific aims of this study were to (1) examine mucosal mast cell counts in the cecum in patient with IBS, and IBD (2) compare MMC between the disease groups. We showed increased MMC count in IBS.

Reduced immunostaining for c-kit receptors in mucosal mast cells in inflammatory bowel disease

2007 ◽  
Vol 22 (12) ◽  
pp. 2338-2343 ◽  
Author(s):  
Ashkan Farhadi ◽  
Ali Keshavarzian ◽  
Jeremy Z Fields ◽  
Shriram Jakate ◽  
Maliha Shaikh ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Mast Cells ◽  
Bowel Disease ◽  
Mucosal Mast Cells ◽  
Inflammatory Bowel

Quantitative assessment of intestinal eosinophils and mast cells in inflammatory bowel disease

1996 ◽  
Vol 28 (1) ◽  
pp. 1-13 ◽  
Author(s):  
S.C. BISCHOFF ◽  
J. WEDEMEYER ◽  
A. HERRMANN ◽  
P.N. MEIER ◽  
C. TRAUTWEIN ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Mast Cells ◽  
Bowel Disease ◽  
Quantitative Assessment ◽  
Inflammatory Bowel

scholarly journals Lymphocyte subpopulations and mast cells intestinal changes as indicators of inflammatory bowel disease in dogs

2019 ◽  
Author(s):  
Andrés Espinoza-Zambrano ◽  
Carlos Manuel González
Keyword(s):  
Inflammatory Bowel Disease ◽  
Mast Cells ◽  
T Lymphocytes ◽  
Lamina Propria ◽  
Bowel Disease ◽  
B Lymphocyte ◽  
Gastrointestinal Symptoms ◽  
Intestinal Barrier Function ◽  
Mucosal Permeability ◽  
Inflammatory Bowel

AbstractInflammatory bowel disease (IBD) is a disease with recurring gastrointestinal symptoms. Lymphocytes and mast cells are proposed as important components in the immunopathology of IBD in dogs. Mast cells depend on degranulation, a process that compromises mucosal permeability and normal intestinal barrier function, which alters the normal inflammatory process by allowing recruitment of lymphocytes in dogs with IBD. In this study, T and B lymphocyte populations and mast cells were examined in situ in 39 intestinal samples of dogs affected by IBD, by immunohistochemistry. Both T lymphocytes and mast cells numbers were significantly higher in the lamina propria of the intestinal wall of dogs with IBD compared with control dogs. Out of the total number of mast cells detected by CD117 expression significantly less cells appear to be granulated according to granule staining with Toluidine Blue, suggesting that an important degranulation process takes place in IBD. Single and double immune staining for tryptase and chymase showed that mast cells can express only one or both enzymes. Tryptase positive cells were significantly higher in number that chymase positive and tryptase/chymase positive cells. T lymphocytes were concentrated mostly at the upper portion of the intestinal villi lamina propria while mast cells were distributed mainly among crypts. These results suggest that populations of T lymphocytes and mast cells play a role in the immunopathology and development of IBD in dogs, also these changes could be helpful as complementary indicators of canine IBD.

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