Characterization of human, mouse, and rat cultures of enteric glial cells and their effect on intestinal epithelial cells

2013 ◽  
Vol 25 (11) ◽  
pp. e755-e764 ◽  
Author(s):  
R. Soret ◽  
S. Coquenlorge ◽  
F. Cossais ◽  
G. Meurette ◽  
M. Rolli-Derkinderen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Glial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Enteric Glial Cells

scholarly journals Enteric glia promote intestinal mucosal healing via activation of focal adhesion kinase and release of proEGF

2011 ◽  
Vol 300 (6) ◽  
pp. G976-G987 ◽  
Author(s):  
Laurianne Van Landeghem ◽  
Julien Chevalier ◽  
Maxime M. Mahé ◽  
Thilo Wedel ◽  
Petri Urvil ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Glial Cells ◽  
Focal Adhesion ◽  
Intestinal Epithelial Cells ◽  
Mucosal Damage ◽  
Mucosal Healing ◽  
Intestinal Epithelial ◽  
Enteric Glia ◽  
Enteric Glial Cells

Wound healing of the gastrointestinal mucosa is essential for the maintenance of gut homeostasis and integrity. Enteric glial cells play a major role in regulating intestinal barrier function, but their role in mucosal barrier repair remains unknown. The impact of conditional ablation of enteric glia on dextran sodium sulfate (DSS)-induced mucosal damage and on healing of diclofenac-induced mucosal ulcerations was evaluated in vivo in GFAP-HSVtk transgenic mice. A mechanically induced model of intestinal wound healing was developed to study glial-induced epithelial restitution. Glial-epithelial signaling mechanisms were analyzed by using pharmacological inhibitors, neutralizing antibodies, and genetically engineered intestinal epithelial cells. Enteric glial cells were shown to be abundant in the gut mucosa, where they associate closely with intestinal epithelial cells as a distinct cell population from myofibroblasts. Conditional ablation of enteric glia worsened mucosal damage after DSS treatment and significantly delayed mucosal wound healing following diclofenac-induced small intestinal enteropathy in transgenic mice. Enteric glial cells enhanced epithelial restitution and cell spreading in vitro. These enhanced repair processes were reproduced by use of glial-conditioned media, and soluble proEGF was identified as a secreted glial mediator leading to consecutive activation of epidermal growth factor receptor and focal adhesion kinase signaling pathways in intestinal epithelial cells. Our study shows that enteric glia represent a functionally important cellular component of the intestinal epithelial barrier microenvironment and that the disruption of this cellular network attenuates the mucosal healing process.

scholarly journals Regulation of intestinal epithelial cells transcriptome by enteric glial cells: impact on intestinal epithelial barrier functions

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 507 ◽  
Author(s):  
Laurianne Van Landeghem ◽  
Maxime M Mahé ◽  
Raluca Teusan ◽  
Jean Léger ◽  
Isabelle Guisle ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Glial Cells ◽  
Intestinal Epithelial Cells ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Barrier Functions ◽  
Enteric Glial Cells

scholarly journals Molecular Characterization of an Apical Early Endosomal Glycoprotein from Developing Rat Intestinal Epithelial Cells

1995 ◽  
Vol 270 (4) ◽  
pp. 1583-1588 ◽  
Author(s):  
Becky A. Speelman ◽  
Katrina Allen ◽  
Tamara L. Grounds ◽  
Marian R. Neutra ◽  
Tomas Kirchhausen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Molecular Characterization ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Developing Rat

scholarly journals Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells

2017 ◽  
Vol 11 (12) ◽  
pp. e0006120 ◽  
Author(s):  
Showgy Y. Ma’ayeh ◽  
Jingyi Liu ◽  
Dimitra Peirasmaki ◽  
Katarina Hörnaeus ◽  
Sara Bergström Lind ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Giardia Intestinalis ◽  
Host Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells ◽  
The Impact

Characterization of isoform expression and subcellular distribution of MYPT1 in intestinal epithelial cells

Gene ◽  
2016 ◽  
Vol 588 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Juan-Min Zha ◽  
Hua-Shan Li ◽  
Yi-Tang Wang ◽  
Qian Lin ◽  
Min Tao ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Subcellular Distribution ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Isoform Expression
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