Morphometric analysis of NADPH diaphorase reactive neurons in a rat model of focal excitotoxic striatal injury

2016 ◽  
Vol 36 (6) ◽  
pp. 527-534 ◽  
Author(s):  
Marco Aurelio M. Freire ◽  
Joanilson S. Guimaraes ◽  
Jose Ronaldo Santos ◽  
Hougelle Simplício ◽  
Walace Gomes-Leal
Keyword(s):  
Morphometric Analysis ◽  
Rat Model ◽  
Nadph Diaphorase ◽  
Striatal Injury

Preliminary attempts to establish a rat model of striatal injury in glutaric acidaemia type I

2004 ◽  
Vol 27 (6) ◽  
pp. 819-824 ◽  
Author(s):  
C. B. R. Funk ◽  
A. N. Prasad ◽  
M. R. Del Bigio
Keyword(s):  
Rat Model ◽  
Type I ◽  
Striatal Injury

Induction of NADPH-diaphorase activity in the hippocampus in a rat model of cerebral ischemia and ischemic tolerance

1994 ◽  
Vol 652 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Hiroyuki Kato ◽  
Kyuya Kogure ◽  
Yong Liu ◽  
Tsutomu Araki ◽  
Yasuto Itoyama
Keyword(s):  
Cerebral Ischemia ◽  
Rat Model ◽  
Ischemic Tolerance ◽  
Nadph Diaphorase ◽  
Diaphorase Activity

Impact of corticosteroid on intestinal injury in a gastroschisis rat model: morphometric analysis

2006 ◽  
Vol 41 (3) ◽  
pp. 547-553 ◽  
Author(s):  
Daniel Guimarães Bittencourt ◽  
Maria Weber Guimarães Barreto ◽  
Willy Marcus Gomes França ◽  
Anderson Gonçalves ◽  
Luis Antônio Violin Pereira ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Rat Model ◽  
Intestinal Injury

Effect of antifibrotic agent silymarin on liver cell regeneration in a rat model of secondary biliary fibrosis: a morphometric analysis

1998 ◽  
Vol 12 (S1) ◽  
pp. S42-S44 ◽  
Author(s):  
Gabriele Boigk ◽  
Hermann Herbst ◽  
Ji‐Dong Jia ◽  
Ernst Otto Riecken ◽  
Detlef Schuppan
Keyword(s):  
Morphometric Analysis ◽  
Rat Model ◽  
Liver Cell ◽  
Cell Regeneration

Superior ovarian nerve (SON) transection leads to stunted follicular maturation: a histomorphologic and morphometric analysis in the rat model

2010 ◽  
Vol 93 (5) ◽  
pp. 1711-1714 ◽  
Author(s):  
Melike Doganay ◽  
Adnan Simsek ◽  
Omer L. Tapisiz ◽  
Baris S. Mulazimoglu ◽  
Nihat Yumusak ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Rat Model ◽  
Follicular Maturation ◽  
Superior Ovarian Nerve

scholarly journals Low-Dose Cyclophosphamide Induces Nerve Injury and Functional Overactivity in the Urinary Bladder of Rats

2021 ◽  
Vol 15 ◽  
Author(s):  
Rui Wang ◽  
Ming Hong ◽  
Jingyi Huang ◽  
Na Zhou ◽  
Yao Zhang ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Mrna Expression ◽  
Nerve Injury ◽  
Morphometric Analysis ◽  
Rat Model ◽  
Low Dose ◽  
Primary Neurons ◽  
Withdrawal Threshold ◽  
Mrna Expression Levels

Aim: This research aimed to investigate the neurotoxicity of low-dose cyclophosphamide (CYP) on the urinary bladder of rats by in vivo and in vitro studies.Methods: To establish CYP-induced cystitis rat model, rats were treated with three intraperitoneal injections of CYP (25 mg/kg) in a week. During treatment, the up-down method was used to assess the mechanical withdrawal threshold. On day 8, urodynamic test and bladder smooth muscle contractility study, including the contraction of bladder strips to electrical field stimulation (EFS, 2–64 Hz), carbachol (CCh, 10–8–10–5 M) and KCl (120 mM), were performed to evaluate the function of bladder function. Body weight and bladder weight were also recorded. Morphometric analysis using an optical microscope and transmission electron microscope was performed to observe the changes of microstructure and submicrostructure of the bladder. The major pelvic neurons were isolated and treated with acrolein (the main CYP metabolite) to assess apoptosis in vitro. RT-PCR assays were used to quantify the mRNA expression levels of Nlrp6, Asc, Casp11 and Casp1 in bladder tissues and primary neurons.Results: After CYP injections, the body weights decreased, but the bladder weights increased in the model group. The mechanical withdrawal threshold of the cystitis model remained at a low level. The morphometric analysis suggested bladder inflammation and neuroinflammation in the bladder of the cystitis rat model. Urodynamic test revealed that, the amplitude, the pressure baseline, the peak pressure and pressure threshold of model rats significantly increased after CYP treatment. The muscle strips of model rats exhibited significantly higher contractility caused by EFS and CCh than the controls. Apoptotic cells appeared at the highest concentration group (100 μM acrolein) after 6 h of acrolein incubation in apoptosis assay of primary neurons. The mRNA expression levels of Nlrp6 and Casp11 were significantly increased in the cystitis rat model and in the acrolein-treated neurons.Conclusions: Low-dose CYP treatment was confirmed to induce nerve injury, which leading to bladder pain and overactive bladder in female rats, and the up-regulation of Nlrp6 and Casp11 may contribute to these pathological changes.

Morphometric Analysis of the Hepatocytes from Fish Exposed to Arsenic

1976 ◽  
Vol 34 ◽  
pp. 282-283
Author(s):  
Elsie M. B. Sorensen
Keyword(s):  
Morphometric Analysis ◽  
Arsenic Concentration ◽  
Ultrastructural Changes ◽  
Sodium Arsenate ◽  
Lepomis Cyanellus ◽  
Mammalian Counterpart ◽  
Intracellular Changes ◽  
Green Sunfish ◽  
Organic Pesticides

The detoxification capacity of the liver is well documented for a variety of substances including ethanol, organic pesticides, drugs, and metals. The piscean liver, although less enzymatically active than the mammalian counterpart (1), contains endoplasmic reticulum with an impressive repertoire of oxidizing, reducing, and conjugating abilities (2). Histopathologic changes are kncwn to occur in fish hepatocytes following in vivo exposure to arsenic (3); however, ultrastructural changes have not been reported. This study involved the morphometric analysis of intracellular changes in fish parynchymal hepatocytes and correlation with arsenic concentration in the liver.Green sunfish (Lepomis cyanellus, R.) were exposed to 0, 30, or 60 ppm arsenic (as sodium arsenate) at 20°C for 1, 2, or 3 week intervals before removal of livers for quantification of the arsenic burden (using neutron activation analysis) and morphometric analysis of ultrastructural alterations. Livers were cut into 1 mm cubes for fixation, dehydration, and embedding.

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