Incidence of Saccharomyces cerevisiae Fungemia in Hospitalized Patients Administered Saccharomyces boulardii Probiotic

Mycoses ◽  
2021 ◽  
Author(s):  
Eric Wombwell ◽  
Bridget Bransteitter ◽  
Lisa R. Gillen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Saccharomyces Boulardii ◽  
Hospitalized Patients

scholarly journals Controle biológico de antracnose em pós-colheita de banana “Maçã” com Saccharomyces spp.

2017 ◽  
Vol 43 (1) ◽  
pp. 49-51 ◽  
Author(s):  
Anderson Luis Heling ◽  
Odair José Kuhn ◽  
José Renato Stangarlin ◽  
Nicanor Pilarski Henkemeier ◽  
Sidiane Coltro-Roncato ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Saccharomyces Boulardii ◽  
Colletotrichum Musae

RESUMO Este trabalho objetivou analisar o controle biológico do Colletotrichum musae em bananas por meio de Saccharomyces cerevisiae e Saccharomyces boulardii. Células de S. cerevisiae foram obtidas a partir do fermento de panificação Fleischmann®. Células de S. boulardii foram obtidas a partir do medicamento Floratil®. Utilizou-se um cacho de banana colhido de área orgânica, os frutos passaram por um processo de assepsia, em seguida foram tratados com células de S. cerevisiae, S. boulardii e ambas as leveduras na concentração de 2 g L-1, após 24 horas inoculou-se o C. musae em três pontos por fruto. Para avaliar-se o efeito da concentração de células no tratamento o processo foi repetido, tratando-se os frutos com concentrações de 0; 0,5; 1; 2; 4 e 8 g L-1 de S. cerevisiae e S. boulardii, avaliou-se a área lesionada, a cada 48 horas, por 14 dias. Também avaliou-se o halo de inibição e a produção de compostos voláteis, ambos in vitro, para analisar se há ocorrência de antagonismo. Observou-se que o tratamento com as leveduras reduz o progresso da doença, e que S. cerevisiae e S. boulardii apresentam maior eficiência na concentração de 5,5 e 6,3 g L-1, respectivamente, apresentando redução de 48% e 35% do progresso da doença, respectivamente. Observou-se a formação de halo de inibição e produção de compostos voláteis, indicando que estas leveduras atuam por meio de antagonismo. Deste modo, estas leveduras são potencias agentes de controle biológico do C. musae.

scholarly journals The effect of probiotic nutrition of Saccharomyces boulardii and Saccharomyces cerevisiae on yield, antioxidant activity and expression of interleukin-6 gene in Holstein calves

2020 ◽  
Vol 45 (4) ◽  
pp. 328-337
Author(s):  
S. Sabooni ◽  
M. Chamani ◽  
A. A. Sadeghi ◽  
M. Amin-Afshar ◽  
N. E. J. Kashan
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Antioxidant Activity ◽  
Interleukin 6 ◽  
Experimental Treatment ◽  
Saccharomyces Boulardii ◽  
Randomized Design ◽  
Significant Difference ◽  
Performance Traits ◽  
Holstein Calves

An experiment was performed to investigate the probiotic effect of Saccharomyces boulardii and Saccharomyces cerevisiae on yield, antioxidant activity, and expression of interleukin-6 gene in Holstein calves. Sixteen calves were divided into 4 dietary treatments with 4 calves/treatment. Experimental treatment diets include 1) control (without probiotic use), 2) milk soluble probiotics based on S. boulardii (1 g/kg), 3) milk soluble probiotics based on S. cerevisiae (1 g/kg) and 4) A mixture of yeast probiotics S. boulardii and S. cerevisiae as a solution in dairy milk (1 g/kg each). The completely randomized design was used in this experiment. The results showed that feeding S. boulardii and S. cerevisiae did not have a significant effect on performance traits compared with control. There was no significant difference between the different treatments for the number of antioxidant enzymes compared to the controls. Superoxide dismutase (SOD) in the treatments used probiotics were higher than the control. The expression of interleukin-6 gene expression was increased significantly (P<0.05) for treatments 3 and 4 on Days 10, 30 and 60 compared to the control. In conclusion, addition of probiotics did not alter performance traits of antioxidant activity, but the S. cerevisaetreatment did increase interleukin-6 gene expression which suggests an effect on the immune system. 

scholarly journals Revisiting the Taxonomic Synonyms and Populations of Saccharomyces cerevisiae—Phylogeny, Phenotypes, Ecology and Domestication

2020 ◽  
Vol 8 (6) ◽  
pp. 903 ◽  
Author(s):  
Ana Pontes ◽  
Mathias Hutzler ◽  
Patrícia H. Brito ◽  
José Paulo Sampaio
Keyword(s):  
Saccharomyces Cerevisiae ◽  
New World ◽  
Current Model ◽  
Distinct Species ◽  
Saccharomyces Boulardii ◽  
Natural Environments ◽  
Wild Populations ◽  
Saccharomyces Diastaticus ◽  
Reference Strains ◽  
Type Strains

Saccharomyces cerevisiae—the most emblematic and industrially relevant yeast—has a long list of taxonomical synonyms. Formerly considered as distinct species, some of the synonyms represent variants with important industrial implications, like Saccharomyces boulardii or Saccharomyces diastaticus, but with an unclear status, especially among the fermentation industry, the biotechnology community and biologists not informed on taxonomic matters. Here, we use genomics to investigate a group of 45 reference strains (type strains) of former Saccharomyces species that are currently regarded as conspecific with S. cerevisiae. We show that these variants are distributed across the phylogenetic spectrum of domesticated lineages of S. cerevisiae, with emphasis on the most relevant technological groups, but absent in wild lineages. We analyzed the phylogeny of a representative and well-balanced dataset of S. cerevisiae genomes that deepened our current ecological and biogeographic assessment of wild populations and allowed the distinction, among wild populations, of those associated with low- or high-sugar natural environments. Some wild lineages from China were merged with wild lineages from other regions in Asia and in the New World, thus giving more resolution to the current model of expansion from Asia to the rest of the world. We reassessed several key domestication markers among the different domesticated populations. In some cases, we could trace their origin to wild reservoirs, while in other cases gene inactivation associated with domestication was also found in wild populations, thus suggesting that natural adaptation to sugar-rich environments predated domestication.

scholarly journals FUNGEMIA POR SACCHAROMYCES CEREVISIAE EM PACIENTE PEDIÁTRICO APÓS TRATAMENTO COM PROBIÓTICO

2017 ◽  
Vol 35 (3) ◽  
pp. 361-364 ◽  
Author(s):  
Mariá Ribas Romanio ◽  
Ligia Augusto Coraine ◽  
Vinicius Pignoti Maielo ◽  
Marcelo Luiz Abramczyc ◽  
Renato Lopes de Souza ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Saccharomyces Boulardii ◽  
Terapia Intensiva

RESUMO Objetivo: Descrever um paciente com infecção de corrente sanguínea associada ao uso de probiótico em criança de um ano de idade e discutir as principais indicações e precauções com o emprego terapêutico desses microrganismos. Descrição do caso: Paciente masculino, um ano de idade, portador de síndrome de Down, em pós-operatório tardio de correção de cardiopatia congênita, com desnutrição grave e internado desde os dois meses de vida em Unidade de Terapia Intensiva Pediátrica. Durante o período de internação, apresentou inúmeras infecções relacionadas à ventilação mecânica, cateteres vasculares e outros dispositivos, com uso prolongado e recorrente de antibióticos de amplo espectro. Evoluiu com diarreia crônica e intolerância alimentar, que culminou com o uso de probiótico (Saccharomyces boulardii) por quatro dias. Dois dias após o término do probiótico, desenvolveu choque séptico, com hemoculturas central e periférica positivas para Saccharomyces cerevisiae. Após tratamento antifúngico (Anfotericina B), houve negativação das culturas. O paciente evoluiu sem complicações clínicas adicionais após o evento. Comentários: Apesar dos benefícios bem documentados do uso de probióticos em algumas situações clínicas, deve-se ter cautela quanto à indicação de uso, preparo e administração do medicamento, além do manuseio seguro dos dispositivos invasivos do paciente.

scholarly journals EFEITO DA TREALOSE NA MANUTENÇÃO DA VIABILIDADE DE CÉLULAS DE LEVEDURAS DESIDRATADAS POR LIOFILIZAÇÃO

1997 ◽  
Vol 54 (3) ◽  
pp. 189-194 ◽  
Author(s):  
A.R. ALCARDE ◽  
L.C. BASSO
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Saccharomyces Boulardii

O presente trabalho teve por objetivo avaliar a influência da trealose endógena e também o uso da trealose como crioprotetor, na preservação da viabilidade celular de leveduras de interesse industrial quando submetidas ao processo de liofilização. Foram utilizadas as cepas TA (M-300-A) e SA (cepa isolada da Usina Santa Adélia S/A) da levedura Saccharomyces cerevisiae e a cepa IZ-1904 da levedura Saccharomyces boulardii, as quais passaram por um tratamento de acúmulo da trealose endógena, através de tratamento térmico a 45oC por duas horas em meio tampão citrato de potássio 2M, pH 4,0, acrescido de 2% de glicose, e, a seguir, foram suspensas em cada uma de três soluções crioprotetoras, que foram: leite desnatado a 10%, sacarose a 10% e trealose a 10%. Em seguida as massas de levedura foram liofilizadas e, aos 0, 10, 40 e 90 dias após o processo de liofilização, foram determinadas suas viabilidades. Para todos os crioprotetores, as culturas de levedura que passaram pelo tratamento de acúmulo da trealose endógena apresentaram maior viabilidade após a liofilização do que aquelas que não passaram pelo tratamento. Dentre os crioprotetores testados, a solução de leite desnatado a 10% foi o que proporcionou melhor crioproteção às células de levedura.

Intracellular Signal Triggered by Cholera Toxin inSaccharomyces boulardii and Saccharomyces cerevisiae

1998 ◽  
Vol 64 (2) ◽  
pp. 564-568 ◽  
Author(s):  
Rogelio L. Brandão ◽  
Ieso M. Castro ◽  
Eduardo A. Bambirra ◽  
Sheila C. Amaral ◽  
Luciano G. Fietto ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cholera Toxin ◽  
Mammalian Cells ◽  
Specific Binding ◽  
Saccharomyces Boulardii ◽  
Yeast Cells ◽  
B Subunit ◽  
Nonfermentable Carbon Source ◽  
A Subunit ◽  
Yeast Surface

ABSTRACT As is the case for Saccharomyces boulardii, Saccharomyces cerevisiae W303 protects Fisher rats against cholera toxin (CT). The addition of glucose or dinitrophenol to cells of S. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for S. cerevisiae. The addition of CT to the same cells also resulted in trehalase activation. Experiments performed separately on the A and B subunits of CT showed that both are necessary for activation. Similarly, the addition of CT but not of its separate subunits led to a cyclic AMP (cAMP) signal in both S. boulardii and S. cerevisiae. These data suggest that trehalase stimulation by CT probably occurred through the cAMP-mediated protein phosphorylation cascade. The requirement of CT subunit B for both the cAMP signal and trehalase activation indicates the presence of a specific receptor on the yeasts able to bind to the toxin, a situation similar to that observed for mammalian cells. This hypothesis was reinforced by experiments with 125I-labeled CT showing specific binding of the toxin to yeast cells. The adhesion of CT to a receptor on the yeast surface through the B subunit and internalization of the A subunit (necessary for the cAMP signal and trehalase activation) could be one more mechanism explaining protection against the toxin observed for rats treated with yeasts.

The Effect of Saccharomyces boulardii Primary Prevention on Risk of Hospital-onset Clostridioides difficile Infection in Hospitalized Patients Administered Antibiotics Frequently Associated With C. difficile Infection

2020 ◽  
Author(s):  
Eric Wombwell ◽  
Mark E Patterson ◽  
Bridget Bransteitter ◽  
Lisa R Gillen
Keyword(s):  
Odds Ratio ◽  
Retrospective Cohort ◽  
Probiotic Strain ◽  
Saccharomyces Boulardii ◽  
Hospitalized Patients ◽  
Clostridioides Difficile ◽  
Clostridioides Difficile Infection ◽  
Case Data ◽  
Reduced Risk ◽  
Hospital Prescribing

Abstract Background Hospital-onset Clostridioides difficile infection (HO-CDI) is a costly problem leading to readmissions, morbidity, and mortality. We evaluated the effect of a single probiotic strain, Saccharomyces boulardii, at a standardized dose on the risk of HO-CDI within hospitalized patients administered antibiotics frequently associated with HO-CDI. Methods This retrospective cohort study merged hospital prescribing data with HO-CDI case data. The study assessed patients hospitalized from January 2016 through March 2017 who were administered at least 1 dose of an antibiotic frequently associated with HO-CDI during hospitalization. Associations between S. boulardii administration, including timing, and HO-CDI incidence were evaluated by multivariable logistic regression. Results The study included 8763 patients. HO-CDI incidence was 0.66% in the overall cohort. HO-CDI incidence was 0.56% and 0.82% among patients coadministered S. boulardii with antibiotics and not coadministered S. boulardii, respectively. In adjusted analysis, patients coadministered S. boulardii had a reduced risk of HO-CDI (odds ratio [OR], 0.57 [95% confidence interval {CI}, .33–.96]; P = .04) compared to patients not coadministered S. boulardii. Patients coadministered S. boulardii within 24 hours of antibiotic start demonstrated a reduced risk of HO-CDI (OR, 0.47 [95% CI, .23–.97]; P = .04) compared to those coadministered S. boulardii after 24 hours of antibiotic start. Conclusions Saccharomyces boulardii administered to hospitalized patients prescribed antibiotics frequently linked with HO-CDI was associated with a reduced risk of HO-CDI.

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