A simple High volume culture technique ‐ Good substitute for Polymerase chain reaction for the detection of Aspergillus species in Broncho Alveolar Lavage samples

Mycoses ◽  
2021 ◽  
Author(s):  
Haritha Subhagan ◽  
Jayanthi Savio ◽  
Priyadarshini Padaki ◽  
Sweta Srivastava ◽  
Pavana Thomas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Volume ◽  
Culture Technique ◽  
Aspergillus Species ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Good Substitute

scholarly journals Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.

2016 ◽  
Vol 40 (1) ◽  
pp. 5-8
Author(s):  
Bashar Sadeq Noomy
Keyword(s):  
Polymerase Chain Reaction ◽  
Brucella Abortus ◽  
Bacterial Culture ◽  
Culture Technique ◽  
Test Results ◽  
Chain Reaction ◽  
Culture Techniques ◽  
Milk Samples ◽  
Polymerase Chain ◽  
Pcr Test

      The aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows. Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion. All of them were positive for rose bengal test. Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus. The specificity of culture techniques was 10%, but its sensitivity was only 21.8%. Beside the cautions in dealing with live Brucella abortus (as culture), it is also less sensitive than PCR, though it is better to use PCR technique in the diagnosis of brucellosis in aborted cows milk.

Evaluation of the Polymerase Chain Reaction for the Detection of Salmonella enteritidis in Experimentally Inoculated Eggs and Eggs from Experimentally Challenged Hens

1996 ◽  
Vol 59 (12) ◽  
pp. 1273-1278 ◽  
Author(s):  
AUDREY P. McELROY ◽  
NOAH D. COHEN ◽  
BILLY M. HARGIS
Keyword(s):  
Polymerase Chain Reaction ◽  
Salmonella Enteritidis ◽  
Room Temperature ◽  
Task Force ◽  
Chemical Reduction ◽  
Culture Technique ◽  
Chain Reaction ◽  
Conventional Culture ◽  
Presumptive Identification ◽  
Polymerase Chain

Current recommendations for identification of Salmonella enteritidis (SE)-contaminated eggs, as outlined by the USDA SE Task Force, require the combination of yolk and albumen from several eggs for room-temperature enrichment for 3 days prior to culture on solid medium. We have previously reported the development of a technique involving enzymatic digestion and chemical reduction of pools of egg albumen allowing for the concentration of low numbers of SE by centrifugation. This technique allowed for detection of Salmonella with sensitivity comparable to conventional culture. Importantly, this technique allowed presumptive identification of SE at least 48 h sooner than conventional culture. We presently describe the use of this technique for the concentration of low numbers of SE in albumen pools for detection by the polymerase chain reaction (PCR). Three experiments performed with experimentally inoculated eggs indicated the PCR to be similar in sensitivity to the room temperature culture procedure for the detection of SE. Additionally, 5 experiments were performed in which hens were experimentally challenged with SE, and eggs were collected at selected times postchallenge. While few positive egg pools were detected by either method, data suggested that the PCR did not falsely identify positive eggs. These experiments provide preliminary evidence that the PCR is comparably sensitive to the room-temperature culture technique, while providing presumptive identification of SE 72 h sooner than conventional culture.

scholarly journals Diagnostic Evaluation of Streptococcus gallolyticus Infection in Patients with Colon Diseases by Polymerase Chain Reaction (PCR) and Culturing Methods

2020 ◽  
Vol 13 (6) ◽  
Author(s):  
Foroogh Eshaghi ◽  
Haniyeh Bashi Zadeh Fakhar ◽  
Masood Ghane ◽  
Javad Shokry
Keyword(s):  
Polymerase Chain Reaction ◽  
Brain Heart Infusion ◽  
Positive Culture ◽  
Culture Method ◽  
Standard Test ◽  
Culture Technique ◽  
Chain Reaction ◽  
Streptococcus Gallolyticus ◽  
Polymerase Chain ◽  
Colon Diseases

Background: Different types of Streptococcus gallolyticus are associated with malignant bowel cancer. Objectives: The aim of this study was to compare two culture and molecular methods in identifying Streptococcus gallolyticus in patients with colon diseases. Methods: A descriptive study was conducted to detect Streptococcus gallolyticus in 55 patients with colon diseases referring to hospitals in Babol and Chalus, Iran. A polymerase chain reaction and culture technique were performed. Detection of Streptococcus gallolyticus after deoxyribonucleic acid (DNA) extraction from designed primers (PCO3, PCO4) was used for SODA gene. From the general culture medium, brain heart infusion (BHI) broth and specific medium for bacterial growth and detection were used. Then, the characteristics of the two methods were evaluated. Results: Of 55 biopsy samples of patients with colon diseases, 3 samples (5.5%) with 95% confidence interval were positive and 52 (94.5%) were reported negative in terms of DNA of Streptococcus gallolyticus. According to the culture test, 9 (16.4%) were positive and 46 (83.6%) were negative for diagnosis of Streptococcus gallolyticus bacteria. Based on the diagnostic agreement between the two methods, the ratio of 9 positive cases of culture method to 3 positive cases by polymerase chain reaction (PCR) method (3.6%) were reported positive both in terms of molecular and positive culture, and 7 (12.7%) out of 9 (16.4%) were negative. To investigate the agreement between the culture and PCR methods, the Kappa test was used, which was statistically significant (P < 0.015). Other studies which have been conducted using the culture method, reported a significant relationship between the family history of colorectal cancer, diabetes, and the presence of Streptococcus gallolyticus bacteria. Conclusions: Considering the advantages, disadvantages, and the characteristics of both methods, none of them can be considered as a comprehensive, standard test at present. The simultaneous use of the two methods is recommended in cases where achieving fast results prevails, or when there is a likelihood of sample infection or late-growing microorganisms.

scholarly journals Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

Mycoses ◽  
2018 ◽  
Vol 61 (6) ◽  
pp. 355-359 ◽  
Author(s):  
Juergen Prattes ◽  
Martin Hoenigl ◽  
Stefanie E.-M. Zinke ◽  
Sven Heldt ◽  
Susanne Eigl ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pilot Study ◽  
Polymerase Chain Reaction Assay ◽  
Aspergillus Species ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals DETEKSI MOLEKULER Mycobacterium tuberculosis DI DAHAK CARA POLYMERASE CHAIN REACTION

2018 ◽  
Vol 15 (1) ◽  
pp. 16
Author(s):  
P. B. Notopuro ◽  
J. Nugraha ◽  
H. Notopuro
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Predictive Value ◽  
Culture Technique ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Conventional Culture ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Sensitivity Specificity

tuberculosis is a chronic infectious disease which is found in developing and developed country. It is one of community healthproblems which become priority in national and international health programs. Microbiologic examination is used to establish thediagnosis of tuberculosis beside clinical examination and radiologic examination. Conventional microscopic and culture examinationhave many limitation ie: such as for example low sensitivity, specificity and need a lot of time. New Molecular technique gives morevalue in sensitivity, specificity and the time for examination. the aim of this study was to know the diagnostic value of PolymeraseChain Reaction for detection of Mycobacterium tuberculosis in sputum. the sputum was collected from twenty eight patients suspectedtuberculosis based on the clinical and radiological examination. the study was performed from September 2006 until July 2007. Wedid the conventional culture technique as a diagnostic gold standard and molecular technique to detect the Mycobacterium tuberculosisin the sputum. For molecular technique, we used Polymerase Chain Reaction (PCR) with a set of IS6110 region primer which is specificfor the Mycobacterium tuberculosis Complex. the sensitivity of PCR with IS6110 region primer is 100% (very high), specificity is 82.4%(high), positive predictive value is 89.7% and negative predictive value is 100%. there was statistically no significant difference betweenthe result of PCR and conventional culture method. Based on the result, the Polymerase Chain Reaction examination with primer IS6110region primer can be used as the screening tool for tuberculosis infection, while the clinician waits for culture result.

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