scholarly journals Molecular identification and antifungal susceptibility profiles of Candida dubliniensis and Candida africana isolated from vulvovaginal candidiasis: A single‐center experience in Iran

Mycoses ◽  
2021 ◽  
Author(s):  
Gholamreza Shokoohi ◽  
Javad Javidnia ◽  
Hossein Mirhendi ◽  
Athar Rasekh Jahromi ◽  
Ali Rezaei‐Matehkolaei ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Antifungal Susceptibility ◽  
Vulvovaginal Candidiasis ◽  
Candida Dubliniensis ◽  
Single Center ◽  
Single Center Experience ◽  
Candida Africana ◽  
Susceptibility Profiles

scholarly journals Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil

2018 ◽  
Vol 12 (7) ◽  
pp. e0006675 ◽  
Author(s):  
Rowena Alves Coelho ◽  
Fábio Brito-Santos ◽  
Maria Helena Galdino Figueiredo-Carvalho ◽  
Juliana Vitoria dos Santos Silva ◽  
Maria Clara Gutierrez-Galhardo ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Rio De Janeiro ◽  
Antifungal Susceptibility ◽  
Clinical Strains ◽  
Susceptibility Profiles

scholarly journals Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yu Shi ◽  
Yuxia Zhu ◽  
Shangrong Fan ◽  
Xiaoping Liu ◽  
Yiheng Liang ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Antifungal Susceptibility ◽  
Vulvovaginal Candidiasis

scholarly journals Molecular Identification and Susceptibility of Clinically RelevantScedosporiumspp. in China

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Hong Wang ◽  
Zhe Wan ◽  
Ruoyu Li ◽  
Qiaoyun Lu ◽  
Jin Yu
Keyword(s):  
Molecular Identification ◽  
Sibling Species ◽  
Antifungal Susceptibility ◽  
Intraspecific Diversity ◽  
Pseudallescheria Boydii ◽  
Clinical Strains ◽  
Susceptibility Profiles

As various new sibling species within theScedosporiumspp. have been described recently, this study was conducted to investigate distribution and antifungal susceptibility profiles of the different species ofScedosporiumspp. in China. Twenty-one clinical strains ofScedosporiumfrom China and two strains from Japan were reidentified by MLSA. The analysis included BT2, CAL, RPB, SOD, and ACT and the combination of the five loci.Pseudallescheria boydiicomplex (17 strains) andS. apiospermum(6 strains) were identified.P. boydiicomplex included four closely related subgroups:P. boydii(9 strains),P. ellipsoidea(6 strains),P. fusoidea(1 strain), andP. angusta(1 strain). There were no significant differences in MICs for neither VOR, POS, nor AMB over all the five species in study. For itraconazole, intraspecific diversity was evident.

scholarly journals Molecular identification and antifungal susceptibility of 186 Candida isolates from vulvovaginal candidiasis in southern China

2015 ◽  
Vol 64 (4) ◽  
pp. 390-393 ◽  
Author(s):  
Xiao-Yu Shi ◽  
Yan-Ping Yang ◽  
Ying Zhang ◽  
Wen Li ◽  
Jie-Di Wang ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Southern China ◽  
Antifungal Susceptibility ◽  
Vulvovaginal Candidiasis

scholarly journals Molecular Characterization and Antifungal Susceptibility of Candida Species Isolated From Vulvovaginitis in Jahrom City, South of Iran

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Gholamreza Shokoohi ◽  
Athar Rasekh-Jahromi ◽  
Kavous Solhjoo ◽  
Arash Hasannezhad ◽  
Sadegh Nouripour-Sisakht ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Candida Species ◽  
Geometric Mean ◽  
Antifungal Susceptibility ◽  
Vulvovaginal Candidiasis ◽  
Vaginal Candidiasis ◽  
Candida Spp ◽  
Mean Values ◽  
Susceptibility Tests ◽  
Identification Techniques

Background: Vulvovaginal candidiasis (VVC) is a significant health issue due to Candida spp. Although Candida albicans is considered a major causative agent of vaginal candidiasis, non-albicans species have increased during previous decades. Objectives: This research aimed at molecular identification and assessing antifungal susceptibility of VVC isolated Candida spp. Methods: A professional physician examined two hundred and ninety-five suspected females with vaginitis. The specimens were collected by sterile cotton swabs. Swabs were inoculated on Sabouraud dextrose agar plates and then incubated for 48 - 72 hours at 35°C. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect all Candida species. Broth microdilution, according to the M27-A3 and M27-S4 CLSI documents, were employed for determining the antifungal susceptibility tests of caspofungin (CAS), voriconazole (VRC), itraconazole (ITC), fluconazole (FLU), clotrimazole (CLO), ketoconazole (KTO), amphotericin B (AMB), and nystatin (NYS). Results: A total of 295 females suspected of vulvovaginal candidiasis were examined. The culture results were positive in 50.5% (149 of 295) of specimens. According to molecular identification techniques, C. albicans 133/149 (89.2%), C. glabrata 8/149 (5.4%), and C. kefyr 2/149 (1.4%) were the main species. A mixed infection of C. albicans and C. glabrata 6/149 (4 %) was detected. The geometric mean values to all Candida strains were in increasing order as the following: CAS, 0.075 µg/mL; VRC, 0.091 µg/mL; ITC, 0.15 µg/mL; AMB, 0.22 µg/mL; CLO, 0.23 µg/mL; KTO, 0.28 µg/mL; NYS, 0.88 µg/mL; FLU, 1.48 µg/mL. Further, the MIC ranges of all Candida isolates to the tested antifungal agents were in increasing order as follows: CAS: 0.031 - 0.25 µg/mL, KTO and ITC: 0.031 - 2 µg/mL, VRC: 0.031 - 4 µg/mL, CLO and AMB: 0.031 - 8 µg/mL, NYS: 0.06 - 4 µg/mL, and FLU: 0.12 - 128 µg/mL. Conclusions: We reported 1 (7.2 %) C. glabrata isolate resistance to FLU and 2 (14.3%) C. glabrata isolates susceptible-dose-dependent (SDD) to CAS. We also reported 6 (4.5%), 5 (3.8%), and 2 (1.5%) C. albicans resistance to ITC, FLU, and AMB, respectively, but 100% C. albicans susceptible to CAS and VRC.

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