Rare/cryptic Aspergillus species infections and importance of antifungal susceptibility testing

Mycoses ◽  
2020 ◽  
Vol 63 (12) ◽  
pp. 1283-1298
Author(s):  
Chi‐Ching Tsang ◽  
James Y. M. Tang ◽  
Haiyan Ye ◽  
Fanfan Xing ◽  
Simon K. F. Lo ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Aspergillus Species

Use of spectrophotometric reading for in vitro antifungal susceptibility testing ofAspergillusspp.

1999 ◽  
Vol 45 (10) ◽  
pp. 871-874 ◽  
Author(s):  
Eric Dannaoui ◽  
Florence Persat ◽  
Marie-France Monier ◽  
Elisabeth Borel ◽  
Marie-Antoinette Piens ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Antifungal Susceptibility Testing ◽  
Aspergillus Species ◽  
National Committee ◽  
Broth Microdilution Method

A comparative study of visual and spectrophotometric MIC endpoint determinations for antifungal susceptibility testing of Aspergillus species was performed. A broth microdilution method adapted from the National Committee for Clinical Laboratory Standards (NCCLS) was used for susceptibility testing of 180 clinical isolates of Aspergillus species against amphotericin B and itraconazole. MICs were determined visually and spectrophotometrically at 490 nm after 24, 48, and 72h of incubation, and MIC pairs were compared. The agreement between the two methods was 99% for amphotericin B and ranged from 95 to 98% for itraconazole. It is concluded that spectrophotometric MIC endpoint determination is a valuable alternative to the visual reference method for susceptibility testing of Aspergillus species.Key words: antifungal, susceptibility testing, Aspergillus, spectrophotometric reading.

scholarly journals Colorimetric Assay for Antifungal Susceptibility Testing of Aspergillus Species

2001 ◽  
Vol 39 (9) ◽  
pp. 3402-3408 ◽  
Author(s):  
J. Meletiadis ◽  
J. W. Mouton ◽  
J. F. G. M. Meis ◽  
B. A. Bouman ◽  
J. P. Donnelly ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Colorimetric Assay ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Aspergillus Species
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