High prevalence of triazole‐resistant Aspergillus fumigatus sensu stricto in an Argentinean cohort of patients with cystic fibrosis

Mycoses ◽  
2020 ◽  
Vol 63 (9) ◽  
pp. 937-941 ◽  
Author(s):  
Tomás Brito Devoto ◽  
Katherine Hermida‐Alva ◽  
Gladys Posse ◽  
Jorge L. Finquelievich ◽  
Guillermo García‐Effrón ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
Sensu Stricto ◽  
High Prevalence

scholarly journals High prevalence of triazole resistance in Aspergillus fumigatus, especially mediated by TR/L98H, in a French cohort of patients with cystic fibrosis

2012 ◽  
Vol 67 (8) ◽  
pp. 1870-1873 ◽  
Author(s):  
F. Morio ◽  
G. G. Aubin ◽  
I. Danner-Boucher ◽  
A. Haloun ◽  
E. Sacchetto ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
High Prevalence ◽  
French Cohort

scholarly journals High Prevalence of Azole-Resistant Aspergillus fumigatus in Adults with Cystic Fibrosis Exposed to Itraconazole

2011 ◽  
Vol 56 (2) ◽  
pp. 869-874 ◽  
Author(s):  
Pierre-Régis Burgel ◽  
Marie-Thérèse Baixench ◽  
Michaël Amsellem ◽  
Etienne Audureau ◽  
Jeanne Chapron ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Previous Treatment ◽  
University Hospital ◽  
Clinical Implications ◽  
Content Type ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
High Prevalence

ABSTRACTAspergillus fumigatusis the most frequent fungus found in the sputum of cystic fibrosis (CF) subjects. Itraconazole is prescribed for allergic bronchopulmonary aspergillosis (ABPA) orAspergillusbronchitis in CF subjects. We hypothesized thatA. fumigatusisolates in the sputum of CF subjects with previous exposure to itraconazole was associated with higher prevalence of azole resistance. From June 2010 to April 2011, sputum samples from adult CF subjects at Cochin University Hospital (France) were examined systematically for the detection ofA. fumigatus. MICs ofA. fumigatusisolates against azoles were screened using Etest, and reduced susceptibility to azoles was confirmed using the CLSI broth microdilution method.A. fumigatuswas isolated from the sputum of 131/249 (52.6%) adult CF subjects, and 47/131 (35.9%) subjects had received previous treatment with itraconazole. ReducedA. fumigatussusceptibility to itraconazole (MIC, ≥2 mg/liter) was confirmed in 6/131 (4.6%) subjects. All 6 isolates also had reduced susceptibility to posaconazole (MIC, ≥0.5 mg/liter), and 3/6 isolates had reduced susceptibility to voriconazole (MIC, ≥2 mg/liter). Mutations in thecyp51Agene were detected at positions previously implicated to cause resistance in 5 isolates. Azole-resistantA. fumigatusisolates were found in 5/25 (20%) subjects exposed to itraconazole within the previous 3 years. High rates of azole-resistantA. fumigatusisolates were present in adult CF subjects and were associated with recent itraconazole exposure. Although the clinical implications of these findings will require further studies, the cautious use of itraconazole in adult CF subjects can be recommended.

Prevalence of Aspergillus fumigatus and other fungal species in the sputum of adult patients with cystic fibrosis

Mycoses ◽  
2003 ◽  
Vol 46 (1-2) ◽  
pp. 19-23 ◽  
Author(s):  
N. Bakare ◽  
V. Rickerts ◽  
J. Bargon ◽  
G. Just-Nübling
Keyword(s):  
Cystic Fibrosis ◽  
Aspergillus Fumigatus ◽  
Fungal Species ◽  
Adult Patients

Biological Parameters of Three Populations of Triatoma dimidiata s. s. (Hemiptera: Reduviidae) From Western Mexico

2021 ◽  
Author(s):  
Benjamin Nogueda-Torres ◽  
Oziel D Montañez-Valdez ◽  
J G Michel-Parra ◽  
Diana M Martínez-Grant ◽  
J Alejandro Martínez-Ibarra
Keyword(s):  
Pacific Coast ◽  
Median Number ◽  
Sensu Stricto ◽  
Human Populations ◽  
Western Mexico ◽  
Post Feeding ◽  
The Pacific ◽  
High Prevalence ◽  
Vector Borne ◽  
Cruzi Infection

Abstract Chagas disease is a very important vector-borne disease in México, and Triatoma dimidiata sensu stricto (Latreille) is one of the most important vectors of Trypanosoma cruzi Chagas, mainly in southern and central states. In the Pacific Coast states with the highest prevalence of human T. cruzi infection, T. dimidiata s. s. is considered as a secondary vector. However, the vectorial capacity of those populations has not been studied. Therefore, the vector characteristics of three populations of T. dimidiata s. s. in western México were evaluated in this study. The populations were maintained in the laboratory at 27 ± 1°C and 75% ± 5% RH with a 12:12 h (light:dark) regime, fed on rabbits in a fortnight basis. The development times were short (172–238 d), and the number of bloodmeals to molt was low (11). Mortality was moderate (36–45%), the onset of feeding was relatively rapid (0.5–1.7 min), and feedings were extended (>15 min). More than 40% of individuals in most instars defecated in one of three categories: <1 min when feeding (5–37.9%), immediately after feeding (9–28.6%), or in <1 min post feeding (7–25.8%). The median number of laid eggs was high (over 2.5) in the three populations, as were the egg eclosion rates (>86%). Thus, the T. dimidiata s. s. in the three populations are potentially efficient vectors of T. cruzi and could contribute to the high prevalence of infection in human populations in western México.

Azole resistance among clinical isolates of Aspergillus fumigatus in Lima-Peru

2019 ◽  
Vol 58 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Beatriz Bustamante ◽  
Luis Ricardo Illescas ◽  
Andrés Posadas ◽  
Pablo E Campos
Keyword(s):  
Aspergillus Fumigatus ◽  
Latin American ◽  
Pcr Amplification ◽  
Sensu Stricto ◽  
Clinical Isolates ◽  
Azole Resistance ◽  
Molecular Testing ◽  
Naive Patient ◽  
In Vitro Susceptibility

Abstract Azole resistance among Aspergillus fumigatus isolates, which is mainly related to mutations in the cyp51A gene, is a concern because it is rising, worldwide disseminated, and associated with treatment failure and death. Data on azole resistance of aspergillus from Latin American countries is very scarce and do not exist for Peru. Two hundred and seven Aspergillus clinical isolates collected prospectively underwent mycology and molecular testing for specie identification, and 143 isolates were confirmed as A. fumigatus sensu stricto (AFSS). All AFSS were tested for in vitro azole susceptibility, and resistant isolates underwent PCR amplification and sequencing of the whole cyp51A gene and its promoter. The in vitro susceptibility showed a minimal inhibitory concentration (MIC) range, MIC50 and MIC90 of 0.125 to >16, 0.25, and 0.5 μg/ml for itraconazole; 0.25 to 2, 0.5, and 0.5 μg/ml for voriconazole; and 0.003 to 1, 0.06, and 0.125 μg/ml for posaconazole. Three isolates (2%) showed resistance to itraconazole and exhibited different mutations of the cyp51A gene. One isolate harbored the mutation M220K, while a second one exhibited the G54 mutation plus a modification in the cyp51A gene promoter. The third isolate, from an azole naive patient, presented an integration of a 34-bp tandem repeat (TR34) in the promoter region of the gene and a substitution of leucine 98 by histidine (L98H). The three source patients had a diagnosis or suspicion of chronic pulmonary aspergillosis.

scholarly journals Effect of Aspergillus fumigatus and Candida albicans on pro-inflammatory response in cystic fibrosis epithelium

2011 ◽  
Vol 10 (6) ◽  
pp. 401-406 ◽  
Author(s):  
James A. Reihill ◽  
John E. Moore ◽  
J. Stuart Elborn ◽  
Madeleine Ennis
Keyword(s):  
Cystic Fibrosis ◽  
Candida Albicans ◽  
Inflammatory Response ◽  
Aspergillus Fumigatus

Impact of Aspergillus fumigatus infection on lung function in children with cystic fibrosis

2021 ◽  
Author(s):  
Ludmila Balanetchi ◽  
Rodica Selevestru ◽  
Mariana Cotorobai ◽  
Diana Rotaru-Cojocari ◽  
Cristina Tomacinschi ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Aspergillus Fumigatus
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