Antidermatophytic activity of synthetic peptides: Action mechanisms and clinical application as adjuvants to enhance the activity and decrease the toxicity of Griseofulvin

Mycoses ◽  
2020 ◽  
Vol 63 (9) ◽  
pp. 979-992 ◽  
Author(s):  
Pedro F. N. Souza ◽  
Patrícia G. Lima ◽  
Cleverson D. T. Freitas ◽  
Daniele O. B. Sousa ◽  
Nilton A. S. Neto ◽  
...  
Keyword(s):  
Clinical Application ◽  
Synthetic Peptides ◽  
Action Mechanisms ◽  
Antidermatophytic Activity

scholarly journals Antibiofilm Activity of Synthetic Peptides Against Candida Albicans and C. Krusei: Action Mechanisms and Clinical Application to Overcome the Resistance Towards Antifungal Drugs Running Title: Antibiofilm Activity of Synthetic Peptides

2021 ◽  
Author(s):  
Leandro P. Bezerra ◽  
Ayrles F.B. Silva ◽  
Jackson L. Amaral ◽  
Nilton A.S. Neto ◽  
Rafael G. G. Silva ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Biofilm Formation ◽  
Synthetic Peptides ◽  
Hydrophobic Interactions ◽  
Resistance Mechanism ◽  
Antifungal Drugs ◽  
Antibiofilm Activity ◽  
Human Pathogens ◽  
Membrane Pore ◽  
Action Mechanisms

Abstract Yeasts belonging to the Candida genus are important human pathogens. Candida biofilm is the most common resistance mechanism, which could increase in 1000 times the resistance to antifungal drugs. This study aimed to evaluate the antibiofilm activity of synthetic peptides, as well as action mechanisms and synergistic effect with Nystatin (NYS) and Itraconazole (ITR) by Scanning Electron Microscopy (SEM) and Fluorescence Microscopy (FM). ITR (1000 µg. mL− 1) inhibited 10% of biofilm formation of C. krusei and NYS (1000 µg. mL− 1) 40% of C. albicans. Regarding synergistic effect, peptides enhance 7-fold the action of ITR to inhibit the biofilm formation of C. krusei and C. albicans, as well as the degradation of formed biofilm of C. krusei. The action mechanism of peptides or in combination with antifungal drugs involved cell wall damage, membrane pore formation, loss of cytoplasmic content, and overproduction of reactive oxygen species (ROS). Docking analysis revealed ionic and hydrophobic interactions between peptides and both drugs, which may explain the synergistic effect. Altogether, our results suggest the high potential of synthetic peptides be employed as adjuvants enhancing the activity of antifungal drugs to overcome the resistance provided by fungal biofilm and decrease the toxicity of drugs.

Immunological Tolerance Using Synthetic Peptides - Basic Mechanisms and Clinical Application

2006 ◽  
Vol 6 (6) ◽  
pp. 631-643 ◽  
Author(s):  
Kristin Hochweller ◽  
Claire Sweenie ◽  
Stephen Anderton
Keyword(s):  
Clinical Application ◽  
Synthetic Peptides ◽  
Immunological Tolerance

Echocardiography. Clinical application in mitral stenosis

JAMA ◽  
1966 ◽  
Vol 195 (3) ◽  
pp. 161-166 ◽  
Author(s):  
B. L. Segal
Keyword(s):  
Clinical Application ◽  
Mitral Stenosis

1776: Prostate Cancer Laterality as a Rationale for the Clinical Application of Focal Ablative Therapy: an Analysis of 1184 Prostatectomy Specimens

2007 ◽  
Vol 177 (4S) ◽  
pp. 590-590
Author(s):  
Thomas J. Polascik ◽  
Vladimir Mouraviev ◽  
Janice M. Mayes ◽  
Leon Sun ◽  
John Madden ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Clinical Application ◽  
Ablative Therapy
Sign in / Sign up

Export Citation Format

Share Document