Role of major facilitator superfamily transporter Qdr2p in biofilm formation by Candida glabrata

Mycoses ◽  
2019 ◽  
Vol 62 (12) ◽  
pp. 1154-1163 ◽  
Author(s):  
Tria Widiasih Widiyanto ◽  
Xinyue Chen ◽  
Shun Iwatani ◽  
Hiroji Chibana ◽  
Susumu Kajiwara
Keyword(s):  
Biofilm Formation ◽  
Candida Glabrata ◽  
Major Facilitator Superfamily ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

scholarly journals The Major Facilitator Superfamily Transporter ZIFL2 Modulates Cesium and Potassium Homeostasis in Arabidopsis

2014 ◽  
Vol 56 (1) ◽  
pp. 148-162 ◽  
Author(s):  
Estelle Remy ◽  
Tânia R. Cabrito ◽  
Rita A. Batista ◽  
Miguel C. Teixeira ◽  
Isabel Sá-Correia ◽  
...  
Keyword(s):  
Major Facilitator Superfamily ◽  
Potassium Homeostasis ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

Tigecycline efflux in Acinetobacter baumannii is mediated by TetA in synergy with RND-type efflux transporters

2020 ◽  
Vol 75 (5) ◽  
pp. 1135-1139 ◽  
Author(s):  
Wuen Ee Foong ◽  
Jochen Wilhelm ◽  
Heng-Keat Tam ◽  
Klaas M Pos
Keyword(s):  
Acinetobacter Baumannii ◽  
Efflux Pump ◽  
Gene Knockout ◽  
Drug Susceptibility ◽  
Major Facilitator Superfamily ◽  
Rt Pcr ◽  
E Coli ◽  
Rnd Efflux Pump ◽  
Major Facilitator

Abstract Objectives To investigate the role of Major Facilitator Superfamily (MFS)-type transporters from Acinetobacter baumannii AYE in tigecycline efflux. Methods Two putative tetracycline transporter genes of A. baumannii AYE (tetA and tetG) were heterologously expressed in Escherichia coli and drug susceptibility assays were conducted with tigecycline and three other tetracycline derivatives. The importance of TetA in tigecycline transport in A. baumannii was determined by complementation of tetA in WT and Resistance Nodulation cell Division (RND) gene knockout strains of A. baumannii ATCC 19606. Gene expression of the MFS-type tetA gene and RND efflux pump genes adeB, adeG and adeJ in A. baumannii AYE in the presence of tigecycline was analysed by quantitative real-time RT–PCR. Results Overproduction of TetA or TetG conferred resistance to doxycycline, minocycline and tetracycline in E. coli. Cells expressing tetA, but not those expressing tetG, conferred resistance to tigecycline, implying that TetA is a determinant for tigecycline transport. A. baumannii WT and RND-knockout strains complemented with plasmid-encoded tetA are significantly less susceptible to tigecycline compared with non-complemented strains. Efflux pump genes tetA and adeG are up-regulated in A. baumannii AYE in the presence of subinhibitory tigecycline concentrations. Conclusions TetA plays an important role in tigecycline efflux of A. baumannii by removing the drug from cytoplasm to periplasm and, subsequently, the RND-type transporters AdeABC and AdeIJK extrude tigecycline across the outer membrane. When challenged with tigecycline, tetA is up-regulated in A. baumannii AYE. Synergy between TetA and the RND-type transporters AdeABC and/or AdeIJK appears necessary for A. baumannii to confer higher tigecycline resistance via drug efflux.

scholarly journals Enhanced Efflux Pump Expression in Candida Mutants Results in Decreased Manogepix Susceptibility

2020 ◽  
Vol 64 (5) ◽  
Author(s):  
Sean D. Liston ◽  
Luke Whitesell ◽  
Mili Kapoor ◽  
Karen Joy Shaw ◽  
Leah E. Cowen
Keyword(s):  
Fungal Pathogens ◽  
Candida Parapsilosis ◽  
Efflux Pump ◽  
Major Facilitator Superfamily ◽  
Transporter Gene ◽  
Transcription Factor Gene ◽  
Content Type ◽  
Atp Binding Cassette Transporter ◽  
Major Facilitator Superfamily Transporter ◽  
Major Facilitator

ABSTRACT Manogepix is a broad-spectrum antifungal agent that inhibits glycosylphosphatidylinositol (GPI) anchor biosynthesis. Using whole-genome sequencing, we characterized two efflux-mediated mechanisms in the fungal pathogens Candida albicans and Candida parapsilosis that resulted in decreased manogepix susceptibility. In C. albicans, a gain-of-function mutation in the transcription factor gene ZCF29 activated expression of ATP-binding cassette transporter genes CDR11 and SNQ2. In C. parapsilosis, a mitochondrial deletion activated expression of the major facilitator superfamily transporter gene MDR1.

scholarly journals γ-Resorcylate Catabolic-Pathway Genes in the Soil Actinomycete Rhodococcus jostii RHA1

2015 ◽  
Vol 81 (21) ◽  
pp. 7656-7665 ◽  
Author(s):  
Daisuke Kasai ◽  
Naoto Araki ◽  
Kota Motoi ◽  
Shota Yoshikawa ◽  
Toju Iino ◽  
...  
Keyword(s):  
Major Facilitator Superfamily ◽  
Pcr Analysis ◽  
Decarboxylase Activity ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Intergenic Regions ◽  
Major Facilitator Superfamily Transporter ◽  
Maleylacetate Reductase ◽  
Major Facilitator ◽  
Rhodococcus Jostii

ABSTRACTTheRhodococcus jostiiRHA1 gene cluster required for γ-resorcylate (GRA) catabolism was characterized. The cluster includestsdA,tsdB,tsdC,tsdD,tsdR,tsdT, andtsdX, which encode GRA decarboxylase, resorcinol 4-hydroxylase, hydroxyquinol 1,2-dioxygenase, maleylacetate reductase, an IclR-type regulator, a major facilitator superfamily transporter, and a putative hydrolase, respectively. ThetsdAgene conferred GRA decarboxylase activity onEscherichia coli. Purified TsdB oxidized NADH in the presence of resorcinol, suggesting thattsdBencodes a unique NADH-specific single-component resorcinol 4-hydroxylase. Mutations in eithertsdAortsdBresulted in growth deficiency on GRA. ThetsdCandtsdDgenes conferred hydroxyquinol 1,2-dioxygenase and maleylacetate reductase activities, respectively, onE. coli. Inactivation oftsdTsignificantly retarded the growth of RHA1 on GRA. The growth retardation was partially suppressed under acidic conditions, suggesting the involvement oftsdTin GRA uptake. Reverse transcription-PCR analysis revealed that thetsdgenes constitute three transcriptional units, thetsdBADCandtsdTXoperons andtsdR. Transcription of thetsdBADCandtsdTXoperons was induced during growth on GRA. Inactivation oftsdRderepressed transcription of thetsdBADCandtsdTXoperons in the absence of GRA, suggesting thattsdgene transcription is negatively regulated by thetsdR-encoded regulator. Binding of TsdR to thetsdR-tsdBandtsdT-tsdRintergenic regions was inhibited by the addition of GRA, indicating that GRA interacts with TsdR as an effector molecule.

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